Platelet count evaluation using three automated haematology analysers compared with the immunoplatelet reference method, and estimation of possible inadequate platelet transfusion

Abstract

The accuracy of three automated haematology analysers [Sysmex XE-2100 (both optical and impedance mode), Bayer Advia 120, and Beckman Coulter LH-750] was compared with the immunoplatelet reference method for platelet measurement. A total of 165 blood specimens were obtained from patients and platelet counts were determined using the four-automated haematology analyser methods and the immunoplatelet reference method. The coefficients of determination (R(2)) between the automated haematology analyser methods and the immunoplatelet reference method for the overall platelet range were >0.98. A bias study, however, showed some disagreement. The use of a coincidence correction calculation for the immunoplatelet method did not improve the correlation between the immunoplatelet method and the automated haematology analyser methods. To estimate the possibility of inadequate platelet transfusion, the number of prophylactic platelet transfusion indications determined by the automated haematology analyser platelet counts were compared with the number of transfusion indications according to the platelet counts determined by the immunoplatelet method. An additional 48 blood specimens were included in this analysis. All of the automated haematology analysers showed some disagreement in the transfusion indications when compared with the immunoplatelet method, suggesting the possibility of inadequate platelet transfusion.

New Fluorescent Method (PLT-F) on Sysmex XN2000 Hematology Analyzer Achieved Higher Accuracy in Low Platelet Counting

“…10,11 So far, however, determination of the number of platelets is hampered by a certain degree of variation and deviation, in particular when obtained using different methods and/or instruments, especially when the low-range counts are considered. 4,[12][13][14] The use of both impedance and optical methods in automated hematology analyzers results in platelet measurement deviations that might be caused by interference from large platelets or cell fragments of a size similar to platelets. 5,7 These deviations might be eliminated by the new Sysmex XN2000 analyzer, which offers an alternative method for platelet counting based on a new fluorescence method combined with an extended counting volume and a prolonged counting time.…”

Evaluation of low platelet counts by optical, impedance, and CD61‐immunoplatelet methods: estimation of possible inappropriate platelet transfusion

“…Overtransfusion may be less interesting to clinicians, since generally it does not raise complications in patients. However, considering increased blood costs and a decrease in blood donations, the possibility of overtransfusion should also be of concern 5,12,26 …”

Study on Platelet Count on the Basis of Red Cell: Platelet Ratio

“…A sudden, unexpected improvement in the platelet count also should be confirmed by blood smear examination, since such an improvement may be factitious 9 . Until recently, the only reference method for platelet counting was the manual phase contrast microscope chamber counts 11 in which platelets are counted manually with a haemocytometer, such as Neubauer chamber. This is laborious, timeconsuming and above all, an imprecise technique.…”