Platelet count is a sensitive predictor of autologous peripheral blood progenitor cell collection yield in previously treated plasma cell disease patients

Abstract

BACKGROUND-It is often a clinical dilemma to determine when to collect autologous peripheral blood progenitor cells (PBPCs) in patients who received prior chemotherapy. It is also challenging to predict if the collected cells will be enough for one or two transplants.

Hematopoietic stem cells from poor and good mobilizers are qualitatively equivalent

“…We also observed in this study that mobilization capacity affected time to platelet engraftment but not time to neutrophil engraftment. Our previous report shows time to platelet engraftment is more responsive to HSC dose than time to neutrophil engraftment 26 . This finding explains the observation that time to platelet engraftment was statistically significantly shorter in high mobilizers.…”

“…23 The quality of collected HPC is defined by proportion of primitive HSC subsets (CD34+CD38−, CD34+HLA-DR−, and CD34+ in G0 stage of cell cycle) 24 , the proportion of HSCs that express CXCR4 and CD26 homing proteins 8,25 , and days to neutrophil and platelet engraftments post transplant. 26 However, the correlation of the quantity and the quality of the HSCs in the autologous BMT setting is unclear. In this study, we tested the hypothesis that HSCs collected from low mobilizers are qualitatively inferior to HSCs from high mobilizers.…”

Factors Associated with Successful Mobilization and Collection of Peripheral Blood Hematopoietic Stem Cells in Autologous and Allogeneic Donors

“…There has been a lot of investigations of predictive factors that could influence mobilization and collection of PBSC in order to improve efficacy and safety of mobilization and harvestration [19][20][21][22][23][24][25][26][27]. There are still a lot of contradictory in the different studies that work on this issue and final conclusions cannot be made, primarily because of heterogeneity of the donors.…”

“…Zubair et al showed that platelet count before growth factor administration significantly correlated with the total CD34+ cell yield (Spearman r = 0.38, p < 0.001). With a multiple linear regression model (adjusted R2 = 0.31, AIC = 63.1), it has been determined that the baseline platelet count significantly correlated with total CD34+ cell yield in treated plasma cell disorder patients in their study [19]. Although there was no significant association between CD34+ cell × 10 6 /kg and blood parameters in the study of Garicoa et al, they found out that leukocyte count higher than 30 × 10 9 /L and monocyte count higher than 1.8 × 10 9 /L could be predictive factors of efficient collection.…”

“…Proper timing of collection of PBSC following mobilization is crucial for maximization of harvest. Few different parameters were investigated as possible predictive factors for apheresis harvest, such as number of platelets [4,19,20], total leukocyte count [20], lymphocyte count [20], monocyte count (20,21) and percentage of circulating immature granulocytes [22]. Number of CD34+ cells in the peripheral blood is accepted as best indicator for initiation of apheresis.…”