Platelet-derived growth factor enhances demineralized bone matrix-induced cartilage and bone formation

Abstract

Subcutaneous implantation of demineralized bone matrix induces the local formation of cartilage and bone. In this study we have investigated the influence of adding various growth factors to the implant. Cartilage formation was monitored by measuring collagen II mRNA levels, and bone formation in the implant was assessed from alkaline phosphatase activity and calcium content. Supplements of the platelet-derived growth factor to implants in older rats increased and production of mRNA for collagen II, alkaline phosphatase activity, and the calcium content of the implant, whereas the other growth factors tested were without effect. The data suggest that under some conditions bone induction is submaximal and can be increased by local supplement of platelet-derived growth factor (PDGF). The present observations may have important therapeutic implications in the treatment of nonunions of fractures and impaired bone formation in the aged.

A new simplified technique for producing platelet-rich plasma: a short technical note

“…PDGF probably enhances the secretion of IGF-I by the osteoblasts and mesenchymal stem cells, which then accelerates the formation of a collagenous matrix [11]. PDGF also seems to enhance the activity of BMP in promoting cartilage and bone formation [10].…”

Do autologous growth factors enhance transforaminal lumbar interbody fusion?

“…None of the variables (age, sex, medical co-morbidity, smoking history, number of levels fused, previous surgery, body mass index, operative time, and estimated blood loss) was significantly related to presence/absence of pseudarthrosis/non-union, with P≥0. 20. There was no 403 (4) 170.9 (4.9) 0.0001 TGF-β (ng/ml) 49.…”

“…Recent studies have reported the efficacy of bone morphogenetic protein (BMP) in facilitating spinal fusion [5]. The beneficial effects of platelet-derived growth factor (PDGF) on bone formation in vitro has also been described previously [9,10,18,20,22,34,36,37], while in vivo stimulation of bone formation by transforming growth factor-β (TGF-β) has been shown in animal models [20,30,33]. Both PDGF and TGF-β have mitogenic effects on fibroblasts, osteoblasts, and mesenchymal cells by the stimulation of DNA synthesis and cell replication [12].…”

Effect of Bone Marrow Cell Collection Techniques and Donor Site Locations on In-vitro Growth of Bone Forming Cells

“…The metaphysis of long bones, such as the tibia 1 and the radius and coronoid processes of the ulna can be local alternative sources near the lesion. Thus, donor site location 9 , harvesting technique 6-8 and age-related decline of osteoprogenitor cells [11][12][13][14] , as well as aspiration volume, can affect in vitro and in vivo growth of bone forming cells. The relationships between these factors are not well understood.…”

“…Aspiration volume from the bone marrow 5 , harvesting technique 6-8 , donor site location, 9 and the agerelated decline [10][11][12][13][14] can affect the in vitro growth of osteoprogenitor cells. Although bone marrow aspirate is the most common, bone marrow cell collection from the ground bone sludge and reaming debris of the long bone can also provide vital mesenchymal stem cells 15 .…”