Platelet-derived growth factor oncoprotein detection using three-dimensional carbon microarrays

Penmatsa, Varun; Ruslinda, A. Rahim; Beidaghi, Majid; Kawarada, Hiroshi; Wang, Chunlei

doi:10.1016/j.bios.2012.06.055

Cited by 34 publications

(28 citation statements)

References 42 publications

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“…We have focused on developing various 3D micro/nanostructures using C-MEMS technique for the last decade [7][8][9][10][11][12][13][14][15][16][17][18][19]. In this study, different functionalization methods on C-MEMS based carbon microstructure have been investigated to form a highly selective, sensitive and reproducible miniaturized bio-sensing platform.…”

Section: Introductionmentioning

confidence: 99%

C-MEMS for bio-sensing applications

Song

Agrawal

Wang

2015

Energy Harvesting and Storage: Materials, Devices, and Applications VI

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Developing highly sensitive, selective, and reproducible miniaturized bio-sensing platforms require reliable biointerface which should be compatible with microfabrication techniques. In this study, we have fabricated pyrolyzed carbon arrays with high surface area as a bio-sensing electrode, and developed the surface functionalization methods to increase biomolecules immobilization efficiency and further understand electrochemical phenomena at biointerfaces. The carbon microelectrode arrays with high aspect ratio have been fabricated by carbon microelectromechanical systems (C-MEMS) and nanomaterials such as graphene have been integrated to further increase surface area. To achieve the efficient covalent immobilization of biomolecules, various oxidation and reduction functionalization methods have been investigated. The oxidation treatment in this study includes vacuum ultraviolet, electrochemical activation, UV/Ozone and oxygen RIE. The reduction treatment includes direct amination and diazonium grafting. The developed bio-sensing platform was then applied for several applications, such as: DNA sensor; H 2 O 2 sensor; aptamer sensor and HIV sensor.

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Section: Introductionmentioning

confidence: 99%

C-MEMS for bio-sensing applications

Song

Agrawal

Wang

2015

Energy Harvesting and Storage: Materials, Devices, and Applications VI

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“…Wang group has focused on developing various 3D micro/nanostructures using carbon microelectromechanical systems (C-MEMS) technique, which involves the pyrolysis of patterned photoresist to build a 3D platform [6][7][8][9][10]. The high aspect ratio with the short transport length of the 3D Max: 5.00 micro/nanostructures exhibited excellent performance for various electrochemical applications such as lithium-ion batteries, biosensors, supercapacitors [11][12][13][14][15][16][17][18][19][20][21][22][23][24]. In addition, the 3D carbon microstructures provide an opportunity to integrate nanostructures and further improve the surface area.…”

Section: Introductionmentioning

confidence: 99%

Micro enzymatic biofuel cells: from theoretical to experimental aspect

Song

Agrawal

Wang

2015

Energy Harvesting and Storage: Materials, Devices, and Applications VI

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Miniaturized enzymatic biofuel cells (EBFCs) that convert biological energy into electrical energy by using enzymemodified electrodes are considered as one of the promising candidates to power the implantable medical devices and portable electronics. However, their low power density and insufficient cell lifetime are two big obstacles to need to be tackled before EBFCs become viable for practical application. In this study, the theoretical simulation of this EBFC system is conducted using finite element analysis from COMSOL 4.3a in terms of cell performance, efficiency and optimum cell configurations. We optimized the electrodes design in steady state based on a three dimensional EBFC chip and studied the effect of orientation of the microelectrode arrays in blood artery. In the experimental part, we demonstrated an EBFC system that used 3D micropillar arrays integrated with graphene/enzyme composites. The fabrication process of this system combined top-down carbon microelectromechanical system (CMEMS) technology to fabricate the 3D micropillar arrays platform and bottom-up electrophoretic deposition (EPD) to deposit graphene/enzyme composite onto the 3D micropillar arrays. The amperometric response of the graphene based bioelectrodes exhibited excellent electrochemical performance and the 3D graphene/enzyme based EBFC generated a maximum power density of 136.3 μWcm -2 at 0.59 V, which is about 7 times of the maximum power density of the bare 3D carbon based EBFC.

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“…Our group has been focusing on developing C-MEMS-based micro/nanostructures [15][16][17][18][19][20][21][22][23], and building C-MEMS-based electrodes for EBFCs [24][25][26][27][28][29][30][31]. One of the most important concerns is to achieve direct electron transfer (DET) between the enzyme reaction site and the electrode.…”

Section: Introductionmentioning

confidence: 99%

Modeling and Simulation of Enzymatic Biofuel Cells with Three-Dimensional Microelectrodes

2014

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Abstract:The enzymatic biofuel cells (EBFCs) are considered as an attractive candidate for powering future implantable medical devices. In this study, a computational model of EBFCs based on three-dimensional (3-D) interdigitated microelectrode arrays was conducted. The main focus of this research is to investigate the effect of different designs and spatial distributions of the microelectrode arrays on mass transport of fuels, enzymatic reaction rate, open circuit output potential and current density. To optimize the performance of the EBFCs, numerical simulations have been performed for cylindrical electrodes with various electrode heights and well widths. Optimized cell performance was obtained when the well width is half of the height of the 3-D electrode. In addition, semi-elliptical shaped electrode is preferred based on the results from current density and resistive heating simulation.

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Platelet-derived growth factor oncoprotein detection using three-dimensional carbon microarrays

Cited by 34 publications

References 42 publications

C-MEMS for bio-sensing applications

C-MEMS for bio-sensing applications

Micro enzymatic biofuel cells: from theoretical to experimental aspect

Modeling and Simulation of Enzymatic Biofuel Cells with Three-Dimensional Microelectrodes

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