Platelet-Derived Products in Veterinary Medicine: A New Trend or an Effective Therapy?

Soares, Carla S.; Babo, Pedro S.; Reis, Rui L.; Carvalho, Pedro P.; Gomes, Manuela E.

doi:10.1016/j.tibtech.2020.07.011

Cited by 27 publications

(46 citation statements)

References 89 publications

(142 reference statements)

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“…Autologous platelet-rich plasma (PRP), a whole blood plasma with a high platelet concentration, is becoming a popular nontraditional therapy in human and veterinary medicine as an alternative to circumvent such problems. This byproduct has been used for its anti-inflammatory, regenerative, and antimicrobial properties [ 14 , 15 , 16 , 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

Intrauterine Blood Plasma Platelet-Therapy Mitigates Persistent Breeding-Induced Endometritis, Reduces Uterine Infections, and Improves Embryo Recovery in Mares

et al. 2021

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Microorganisms, including pathogenic or opportunistic bacteria and fungi, may gain access to the uterus during breeding, and infectious endometritis plays a major role in equine subfertility. This study aimed to assess the post-breeding inflammatory response, endometrial culture, and embryo recovery of mares susceptible to persistent breeding-induced endometritis (PBIE) treated with plasma-rich (PRP) or -poor (PPP) plasma. Mares (n = 12) susceptible to PBIE had three cycles randomly assigned to receive intrauterine infusions of lactate ringer solution (LRS, control), or autologous PRP or PPP pre- (−48 and −24 h) and post-breeding (6 and 24 h). Mares were bred with fresh semen from one stallion. Intrauterine fluid accumulation (IUF) and endometrial neutrophils were assessed every 24 h up to 96 h post-breeding. Uterine cytokines (Ilβ, IL6, CXCL8, and IL10) were evaluated before (0 h), 6, and 24 h post-breeding, and endometrial culture three and nine days after breed. Embryo flushing was performed 8 days post-ovulation. Data were analyzed with mixed model, Tukey’s post-hoc test, and multivariate regression. PRP treatment reduced endometrial neutrophils, post-breeding IUF, and pro-inflammatory cytokines when compared to control-assigned cycles, but not significantly different than PPP. Controls had a significantly higher percentage of positive bacterial cultures (33%) in comparison to PRP-assigned cycles (0%), whereas cycles treated with PPP were not significantly different from the other groups (25%). The PRP-assigned cycles had significantly greater embryo recovery rates (83%) than the control (33%), though not significantly different than PPP (60%). Plasma infusion reduced the duration and intensity of the post-breeding inflammatory response and improved embryo recovery in mares susceptible to PBIE. Platelets incrementally downregulate PBIE and appear to have a dose-dependent antimicrobial property.

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Section: Introductionmentioning

confidence: 99%

Intrauterine Blood Plasma Platelet-Therapy Mitigates Persistent Breeding-Induced Endometritis, Reduces Uterine Infections, and Improves Embryo Recovery in Mares

et al. 2021

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“…Expansion of harvested BM-MSCs and the subsequent use of subcultured (or passaged) BM-MSCs were common in most studies (Agis et al, 2009;Amable et al, 2014;Bernardi et al, 2017;Bernardi et al, 2013;Dohan Ehrenfest et al, 2010;Goedecke et al, 2011;Gottipamula et al, 2012;Gruber et al, 2004;Infante et al, 2017;Jonsdottir-Buch et al, 2013;Kosmacheva et al, 2014;Lange et al, 2012;Liou et al, 2018;Lucarelli et al, 2003;Lucarelli et al, 2010;Moisley et al, 2019;Moradian et al, 2017;Muraglia et al, 2015;Murphy et al, 2012;Nguyen et al, 2019;Parsons et al, 2008;Prins et al, 2009;Samuel et al, 2016;Schar et al, 2015;Skific et al, 2018;Verrier et al, 2010;Vogel et al, 2006;Yin et al, 2016), since larger quantities of BM-MSCs were desired for laboratory assays. However, studies have demonstrated the 'internalisation' of bovine proteins in BM-MSCs expanded in culture medium containing FBS, a commonly used animal serum (Spees et al, 2004). The stemness of passaged BM-MSCs and, therefore, how closely it reflects fresh BM-MSCs used in clinical practice is questionable.…”

Section: Discussionmentioning

confidence: 99%

“…Physical disruption of PRP, such as repeated freeze-thaw cycles and ultrasound sonification, results in platelet lysis and, therefore, acellular PL rich in growth factors. Chemical activation of PRP (commonly with calcium salts or thrombin) leads to the production of two distinct products, due to two distinct processes that occur during activation: (i) platelet degranulation and the release of a plasmatic fraction rich in growth factors, known as platelet releasate or PRGF or platelet released supernatants; (ii) fibrinogenesis leading to formation of a platelet-rich clot, also known as PG (Soares et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

The in vitro effects of platelet products on the biophysiological functions of human bone marrow mesenchymal stromal cells: a systematic review

Vun¹,

Panteli²,

Jones³

et al. 2021

eCM

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Platelet products (PP) and bone-marrow aspirate are popular sources of osteoinductive signalling molecules and osteogenic bone marrow mesenchymal stromal cells (BM-MSCs) used in the treatment of impaired bone healing. However, the combined use of PP and BM-MSCs in clinical studies has reported mixed results. Understanding the cellular and molecular interactions between PP and BM-MSCs plays the important role of guiding future research and clinical application. This systematic review investigates the effects of PP on the biophysiological functions of BM-MSCs in in vitro human studies, including (i) proliferation, (ii) migration, (iii) differentiation, (iv) growth factor/cytokine/protein expression, (v) immunomodulation, (vi) chemotactic effect on haematopoietic stem cells, (vii) response to apoptotic stress, and (viii) gene expression. In vitro studies in human have demonstrated the multi-faceted ‘priming effect’ of PP on the biophysiological functions of BM-MSCs. PP has been shown to improve proliferation, migration, osteogenic differentiation, reaction to apoptotic stress as well as immunomodulatory, pro-angiogenic and pro-inflammatory capacities of BM-MSCs. Several factors are highlighted that restrict the transferability of these findings into clinical practice. Therefore, more collaborative in vitro research in humans modelled to reflect clinical practice is required to better understand the effects of PP exposure on the biophysiological function(s) of BM-MSCs in human.

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“…It goes without saying that veterinary medicine offers tremendous potential for gas plasma-supported wound healing. Defective wound healing is a prominent issue across several disciplines of this field [ 140 , 141 ], such as farming, pets, and animals used in sports, e.g., racing horses and camels. The economic impact of non-healing wounds is not to be underestimated, as these often require isolation of the animals, repeated visits by veterinarians, and in the case of sports, a lack of participation in competitive events.…”

Section: Opportunities Of Gas Plasma In Wound Healing Science and Veterinary Medicinementioning

confidence: 99%

Gas Plasma-Augmented Wound Healing in Animal Models and Veterinary Medicine

2021

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The loss of skin integrity is inevitable in life. Wound healing is a necessary sequence of events to reconstitute the body’s integrity against potentially harmful environmental agents and restore homeostasis. Attempts to improve cutaneous wound healing are therefore as old as humanity itself. Furthermore, nowadays, targeting defective wound healing is of utmost importance in an aging society with underlying diseases such as diabetes and vascular insufficiencies being on the rise. Because chronic wounds’ etiology and specific traits differ, there is widespread polypragmasia in targeting non-healing conditions. Reactive oxygen and nitrogen species (ROS/RNS) are an overarching theme accompanying wound healing and its biological stages. ROS are signaling agents generated by phagocytes to inactivate pathogens. Although ROS/RNS’s central role in the biology of wound healing has long been appreciated, it was only until the recent decade that these agents were explicitly used to target defective wound healing using gas plasma technology. Gas plasma is a physical state of matter and is a partially ionized gas operated at body temperature which generates a plethora of ROS/RNS simultaneously in a spatiotemporally controlled manner. Animal models of wound healing have been vital in driving the development of these wound healing-promoting technologies, and this review summarizes the current knowledge and identifies open ends derived from in vivo wound models under gas plasma therapy. While gas plasma-assisted wound healing in humans has become well established in Europe, veterinary medicine is an emerging field with great potential to improve the lives of suffering animals.

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Platelet-Derived Products in Veterinary Medicine: A New Trend or an Effective Therapy?

Cited by 27 publications

References 89 publications

Intrauterine Blood Plasma Platelet-Therapy Mitigates Persistent Breeding-Induced Endometritis, Reduces Uterine Infections, and Improves Embryo Recovery in Mares

Intrauterine Blood Plasma Platelet-Therapy Mitigates Persistent Breeding-Induced Endometritis, Reduces Uterine Infections, and Improves Embryo Recovery in Mares

The in vitro effects of platelet products on the biophysiological functions of human bone marrow mesenchymal stromal cells: a systematic review

Gas Plasma-Augmented Wound Healing in Animal Models and Veterinary Medicine

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