Platelet function and blood coagulation system status in childhood essential thrombocythemia

Polokhov, Dmitry M.; Ершов, Н. М.; Ignatova, Anastasia A.; Пономаренко, Е А; Gaskova, Marina; Zharkov, P. A.; Федорова, Д. В.; Poletaev, A. V.; Seregina, E.A.; Novichkova, Galina; Сметанина, Наталия Сергеевна; Panteleev, Mikhail A.

doi:10.1080/09537104.2019.1704710

Cited by 13 publications

(8 citation statements)

References 27 publications

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“…19 The α value reflects the rate of blood clot formation and reinforcement in vivo, while MA value reflects the function of platelet aggregation. 20,21 Polokhov DM study 22 pointed out that with the enhancement of platelet aggregation functions, patients' coagulation function increases and fibrinolytic capacity decreases. This may easily lead to thrombosis in villous vessels and uterine spiral arteries in pregnant women, resulting in damage to vascular endothelium and embolism.…”

Section: Discussionmentioning

confidence: 99%

Analysis of blood coagulation indexes, thromboelastogram and autoantibodies in patients with recurrent pregnancy loss

Yao¹,

Ji²,

Zhou

2022

Pak J Med Sci

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Objectives: Changes in coagulation indexes, thromboelastogram(TEG) and autoantibodies in patients with recurrent pregnancy loss (RPL) with different number of abortions were analyzed. Methods: Medical records of 48 patients with recurrent abortion, treated in Quzhou people’s Hospital from November 2019 to October 2020, were collected as the observation group. Based on the number of abortions, patients were divided into Group-A (Two abortions, n=21), Group-B (Three abortions, n=16) and group C (Abortion ≥ four times, n=11). Records of 50 healthy pregnant women in our hospital in the same period were selected as the control group. Coagulation indexes [prothrombin time (PT), activated partial prothrombin time (APTT), fibrinogen (FIB), D-Dimer (DD)], thromboelastogram (TEG) parameters [reaction time (R), coagulation time(K), maximum thrombus amplitude (MA), coagulation angle (α)], changes in the levels of autoantibodies [anticardiolipin antibody (ACA), anti-endometrial antibody (EmAb), anti-thyroid antibody(ATA)] were compared between the groups. Results: There were significant differences in the levels of ATPP, Pt, FIB and DD among the groups. Higher number of abortions correlated with lower the levels of ATPP and Pt, and higher levels of FIB and DD (P<0.05). Compared to the control group, R and K in Group-A,B and C decreased, while α and MA increased (P<0.05). There were significant differences in α and MA indexes. The positive rates of ACA, EmAb and ATA in Group-A were higher than those in the control group, but the difference was not statistically significant (P>0.05), while the above indexes in groups B and C were significantly higher than those in the control group(P<0.05). The positive rates of ACA and ATA in group C were significantly higher than those in Group-A(P<0.05), but there was no significant difference in the positive rate of EmAb (P>0.05). Conclusion: RPL was related to the decrease of APTT, PT, and the increase of FIB and DD levels. TEG indicated that the increase of α and MA values indicated that the risk of multiple abortion was increased. The positive rates of ACA, EmAb and ATA were closely related to multiple abortions, especially the positive rates of ACA and ATA. doi: https://doi.org/10.12669/pjms.38.7.6284 How to cite this:Yao H, Ji Y, Zhou Y. Analysis of blood coagulation indexes, thromboelastogram and autoantibodies in patients with recurrent pregnancy loss. Pak J Med Sci. 2022;38(7):2005-2010. doi: https://doi.org/10.12669/pjms.38.7.6284 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Section: Discussionmentioning

confidence: 99%

Analysis of blood coagulation indexes, thromboelastogram and autoantibodies in patients with recurrent pregnancy loss

Yao¹,

Ji²,

Zhou

2022

Pak J Med Sci

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“…ПЦ выполнялась по описанной ранее методике [10][11][12]. Изучался маркерный состав тромбоцитов в состоянии покоя и после активации смесью TRAP-6 (агонист рецептора PAR-1) и CRP (агонист ГП VI).…”

Section: проточная цитометрияunclassified

Age-dependent changes in platelet function of a patient with <i>SLFN14</i>-related macrothrombocytopenia

Rashevskaya,

Polokhov,

Fyodorova

et al. 2023

Voprosy gematologii/onkologii i immunopatologii v pediatrii

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Platelet-type bleeding disorder-20 is a rare inherited thrombocytopenia caused by mutations in the SLFN14 gene. We report a case of a female patient with SLFN14 mutation, macrothrombocytopenia, severe hemorrhagic syndrome and a positive family history who was followed up from the age of 17 to 19. The 3-year follow-up showed a tendency towards partial normalization of platelet counts (from 47 to 82 × 109/L) and morphology. Platelet size and granularity as well as the density of glycoprotein (GP) membrane receptors such as GP Ib/V/IX and GP IIb/IIIa decreased. GP IIb/IIIa activation was impaired and there were no positive changes over time. The dense granules indicators were stably elevated. The parameters of a-granules (assessed by P-selectin expression) did not differ from the control group. The proportion of procoagulant phosphatidylserine-positive platelets at rest was increased and the potential to form procoagulant platelets upon activation was reduced. As the patient grew older, her bleeding disorder symptoms abated and she showed a tendency towards normalization of platelet laboratory parameters. All investigations were performed after obtaining informed consent from the patient and her parents in accordance with the Declaration of Helsinki.

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“…Подходы к выявлению нарушений функции тромбоцитов у детей осложняются недостаточной изученностью возраст-зависимых различий. В частности, есть сообщения о том, что функциональные маркеры тромбоцитов, определяемые методом проточной цитометрии, могут быть значительно уменьшены [7,[18][19][20] или увеличены [20,21] у детей по сравнению со взрослыми.…”

Section: литератураunclassified

Specifities of the storage pool and morphology of platelets in children with unspecified hemorrhagic syndrome

Polokhov

Pshonkin

Ignatova

et al. 2021

Voprosy gematologii/onkologii i immunopatologii v pediatrii

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Despite modern possibilities of laboratory diagnosis of hemorrhagic syndrome, in some patients, the causes of bleeding remain unspecified. Among these reasons, mild defects in the platelet link of hemostasis can potentially be hidden. The aim of the work is to identify the features of the function of the platelet hemostasis in children with unspecified hemorrhagic syndrome. This study is supported by the Independent Ethics Committee and approved by the Academic Council of the Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology. We examined 50 patients aged 2 to 17 years with various manifestations of bleeding and lack of laboratory data proving coagulopathy and/or thrombocytopenia; platelet cytofluorometry with activation was performed. The morphological characteristics of platelets in terms of size/granularity (FSC/SSC), the density of the CD62p receptor as a marker of a-granule secretion, and d-granules of platelets were assessed by the fluorescence of loaded mepacrine. Platelet activation was performed with a CRP + TRAP mixture. Comparison was carried out with the results of examination of 50 healthy children (control group - CG) aged 2 to 17 years. The severity of hemorrhagic syndrome was assessed using the standardized ISTH BAT score. The severity of hemorrhagic manifestations according to BAT ISTH score ranged from 2 to 6 points. As a result of the study, two groups of patients differing in the calculated parameter of the FSC/SSC ratio for non-activated platelets were identified. In the CG, the median FSC/SSC was 1.235 (from 1.1 to 1.4), in group 1 (n = 19), the median was 0.97 (from 0.9 to 1.05), and in group 2 (n = 31), the median was 1.24 (from 1.11 to 1.43). The number of platelets of the CG and the groups of patients did not differ significantly. A significant correlation between a decrease in the number of platelets and an increase in their size and granularity, while maintaining a high correlation between size and granularity was observed in groups of patients. In group 1, the overall granularity was increased regardless of the size and number of platelets, the volume of dense granules and membrane CD62p was increased, but the granular CD62p was decreased. The degranulation mechanism was not impaired in both groups of patients. Our results indicate convincingly the contribution of the storage pool and platelet morphology disorders to the development of hemorrhagic manifestations in children with unspecified hemorrhagic syndrome.

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Platelet function and blood coagulation system status in childhood essential thrombocythemia

Cited by 13 publications

References 27 publications

Analysis of blood coagulation indexes, thromboelastogram and autoantibodies in patients with recurrent pregnancy loss

Analysis of blood coagulation indexes, thromboelastogram and autoantibodies in patients with recurrent pregnancy loss

Age-dependent changes in platelet function of a patient with <i>SLFN14</i>-related macrothrombocytopenia

Specifities of the storage pool and morphology of platelets in children with unspecified hemorrhagic syndrome

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