Platelet Function in Platelet Concentrates and in Whole Blood

Abstract

Platelet function was studied in CPD whole blood stored at 4QC for one and three days and in platelet concentrates stored at room temperature for the same periods of time. Comparisons were made of platelet shape, nucleotide content, (3-thromboglobulin ((3TO) liberated during storage, and platelet aggregation in response to ADP, collagen, sodium arachidonate and ristocetin. It was found that in whole blood the shape of the platelets was less discoid than in platelet concentrates. However, platelet aggregation in response to ADP, collagen, and sodium arachidonate was preserved better in whole blood than in platelet concentrates. Platelet nucleotides were the same in whole blood as in platelet concentrates, but the plasma levels of (3 TO were less in whole blood. The results show that as judged by aggregation, (3 TO release and nucleotide content, platelets from whole blood were at least as functional as those from platelet concentrates. However, platelets from whole blood had lost their discoid shape, which suggests that they would have a short survival in the circulation.

The effects of storage on platelet function in different blood products

“…Further, PLT counts decreased significantly during storage in the studies of Jobes et al [21] and Pidcoke et al [22] without any correction of the results for this confounder. Further, Kornblith et al [24] compared fresh WB to expired PLT concentrates and Rodgers et al [23] only examined up till 3 days of storage, thereby making direct comparisons with the current study difficult.…”

“…Nonetheless, the PLT aggregation capacity of WB is at least as good as it is in BCP or AP. Previous studies have shown reduced PLT aggregation capacity in BCP and AP during storage as measured by light transmission aggregometry [12][13][14][15][16][17][18] or multiple electrode aggregometry [12,13,19,20], but only few have compared PLT function in BCP or AP to that of WB [23,24]. Knowledge of PLT function in WB during storage is sparse and has shown conflicting results [21][22][23].…”

“…Several studies have shown that PLT function tends to decrease during storage at room temperature [12][13][14][15][16][17][18][19][20]. A few studies have examined PLT activity in WB and found contradicting results depending on the agonist used [21][22][23]. Further, PLT function in WB during storage has been shown superior than that of BCP [23,24].…”

“…A few studies have examined PLT activity in WB and found contradicting results depending on the agonist used [21][22][23]. Further, PLT function in WB during storage has been shown superior than that of BCP [23,24]. However, these studies assessed PLT function by methodologies that are known to be dependent on the PLT count or the medium in which the PLTs are suspended [25,26].…”

Response to platelet‐activating factor in human platelets stored and aged in plasma. Decrease in aggregation, phosphoinositide turnover, and receptor affinity

“…let-activating factor (PAF). I 5 * l 6 For instance, PAF causes a rapid turnover of phosphoinositides in platelets, which then leads to platelet aggregation and serotonin secretion. I7-I9 In the present investigation, we monitored the phosphoinositide turnover in PRP because we thought it relevant to study platelet responses in a milieu closer to that in which platelets exist in vivo.…”