Platelet function testing during 5-day storage of single and random donor plateletpheresis

Akay, Olga Meltem; Gündüz, Eren; Başyiğit, Hatice; Gülbaş, Zafer

doi:10.1016/j.transci.2007.03.008

Cited by 19 publications

(21 citation statements)

References 15 publications

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“…10 Similarly, another study reported that platelet aggregation response towards ADP and collagen activation decrease gradually from day 1 to day 5, from 78% to 17%. 16 Contrary to those reports, this study observed that platelets have no measurable response to ADP-induced aggregation on day 3, whilst, from earlier reports, the activity was still measurable until day 5. Having said that, this study has provided evidence that on day 3, ADP-induced platelet aggregation activity was similar to that shown by platelets without ADP, which indicates the incapability of platelets to react to ADP activation.…”

Section: Discussioncontrasting

confidence: 99%

Effect of 3-Day Storage and Temperature on ADP-induced Platelet Aggregation

Puspita

Suryawati

2018

IJIHS

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Objective: To observe platelet functions during the 3 days of platelet storage and to evaluate the effect of storage temperature towards platelet aggregation response.Methods: Two conditions of platelet storage were used in this study: at room temperature and in a cold condition at 4 °C to observe the temperature effect on platelet aggregation. The aggregation test was performed using a 96-wells plate platelet aggregation method.Results: At room temperature on day 1, the response of platelet aggregation reduced to 34.9±10.6%, which was less than half of the aggregation of fresh platelet (100%). The aggregation on day 2 reduced modestly (26.8±4.2%), in comparison with that seen on the first day. On day 3, platelet aggregation deteriorated significantly (6±1.2%), which was comparable to the control group without the presence of platelet agonist (6.1±1.4%). When platelets are stored at 4 °C, the aggregation response to ADP stimulation was slightly different to that shown by platelets stored at room temperature (22 °C). At the 4 °C, the aggregation response was 68.11±34.2%, 57.1±21.4%, and 5.9±2.6% at day 1, day 2, and day 3, respectively. The result showed that storing the blood at 4 ˚C or room temperature was not able to preserve platelet function, as the deterioration of platelet aggregation response over time was still taking place. Although was not statistically significant, our result indicated that cold storage reduced platelet responsiveness toward ADP activation. Conclusions:This current study provides evidence of the deterioration of platelet function during first 3 days of platelet storage. Moreover, we found that storing platelets in 4 °C showed no significant benefit in preserving the ADP-induced platelet aggregation capacity compared to that stored in 22 °C.

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Section: Discussioncontrasting

confidence: 99%

Effect of 3-Day Storage and Temperature on ADP-induced Platelet Aggregation

Puspita

Suryawati

2018

IJIHS

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“…However, unlike preservation of metabolic function, PLT aggregation has consistently been shown to decrease over storage in both random-donor and LR-ADP products. [8][9][10][11] While these reports have exclusively used single agonists for PLT function testing, it has been argued that using pairs of agonists during in vitro function testing may optimally recreate in vivo conditions as physiologically PLT aggregation occurs in response to the synergistic action of a combination of agonists at sites of injury. 12,13 As a result, we deliberately decided to use a mixture of ADP and collagen as agonist.…”

Section: Table 1 Metabolic Characteristics Of Residual Volumes Of Lrmentioning

confidence: 99%

Effects of storage duration and volume on the quality of leukoreduced apheresis‐derived platelets: implications for pediatric transfusion medicine

Winkler

Sheppard²,

Culler³

et al. 2010

Transfusion

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Background-Platelet storage adversely affects platelet structure and function in vitro, and is associated with decreased platelet recovery and function in vivo. In pediatric transfusion medicine, it is not uncommon for small residual volumes to remain in parent units following aliquot preparation of leukoreduced apheresis-derived platelets (LR-ADP). However, limited data exists regarding the impact of storage on residual small volume LR-ADP.

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“…During the storage period of 5 days, platelet aggregation to ADP (5 µM) was decreased from 60–70% at baseline to 24–30% by day 3, and 13–25% by day 5 using 3 different commercial platelet storage bags [66]. Indeed, transfusion of 3-day-old autologous apheresis platelets (2 units) in the subjects who received 3-day course of aspirin plus clopidogrel did not normalize ADP (20 µM)-induced platelet aggregation on LTA, but only restored it to 31–38% (normal, 70%) in 24 h [67].…”

Section: Platelet Transfusion and Adjunct Therapiesmentioning

confidence: 99%

Perioperative management and monitoring of antiplatelet agents: a focused review on aspirin and P2Y₁₂inhibitors

Mazzeffi

Lee

Taylor

et al. 2017

Korean J Anesthesiol

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Platelets play pivotal roles in hemostasis as well as pathological arterial thrombosis. The combination of aspirin and a P2Y12 inhibitor has become the mainstay therapy in the ageing population with cardiovascular conditions, particularly during and after percutaneous coronary intervention. A number of novel P2Y12 inhibitors has become available in the recent years, and they markedly vary in pharmacokinetic and pharmacodynamic properties. Perioperative physicians today face a challenge of preventing hemorrhage due to platelet inhibitors, while minimizing thrombotic risks. There are several point-of-care platelet function tests available in the peri-procedural assessment of residual platelet aggregation. However, these platelet function tests are not standardized in terms of sample processing, agonist type and potency as well as methods of detecting platelet activity. Understanding the differences in pharmacological properties of antiplatelet agents, principles of platelet function tests, and pertinent hemostatic strategies may be useful to anesthesiologists and intensivists who manage perioperative issues associated with antiplatelet agents. The objectives of this review are: 1) to discuss clinical data on aspirin and P2Y12 inhibitors relating to perioperative bleeding, 2) to outline different features of point-of-care platelet function tests, and 3) to discuss therapeutic options for the prevention and treatment of bleeding associated with antiplatelet agents.

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Platelet function testing during 5-day storage of single and random donor plateletpheresis

Cited by 19 publications

References 15 publications

Effect of 3-Day Storage and Temperature on ADP-induced Platelet Aggregation

Effect of 3-Day Storage and Temperature on ADP-induced Platelet Aggregation

Effects of storage duration and volume on the quality of leukoreduced apheresis‐derived platelets: implications for pediatric transfusion medicine

Perioperative management and monitoring of antiplatelet agents: a focused review on aspirin and P2Y₁₂inhibitors

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Platelet function testing during 5-day storage of single and random donor plateletpheresis

Cited by 19 publications

References 15 publications

Effect of 3-Day Storage and Temperature on ADP-induced Platelet Aggregation

Effect of 3-Day Storage and Temperature on ADP-induced Platelet Aggregation

Effects of storage duration and volume on the quality of leukoreduced apheresis‐derived platelets: implications for pediatric transfusion medicine

Perioperative management and monitoring of antiplatelet agents: a focused review on aspirin and P2Y12inhibitors

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Perioperative management and monitoring of antiplatelet agents: a focused review on aspirin and P2Y₁₂inhibitors