Platelet glycolytic flux increases stimulated by ultraviolet‐induced stress is not the direct cause of platelet morphology and activation changes: possible implications for the role of glucose in platelet storage

Abstract

Increased glycolytic flux is not a direct cause for PLT morphology change and spontaneous activation during storage lesion development. Reduction of glucose utilization may increase PLT loss during storage.

In vivo viability of stored red blood cells derived from riboflavin plus ultraviolet light–treated whole blood

“…Na + /K + exchange was affected by treatment, probably through transporter loss of function, similar to results observed with gamma‐irradiated cells 24 . Treatment was also associated with increased lactate production and glucose consumption, suggesting metabolic activation as reported for PRT‐treated PLTs 25 . As expected, the hemolysis level significantly correlated with the 24‐hour recovery of RBCs but not with the t 1/2 survival, suggesting that both hemolysis and 24‐hour recovery measured the destruction of the same acutely injured cell population.…”

Pathogen Reduction Technology Treatment of Platelets, Plasma and Whole Blood Using Riboflavin and UV Light

“…1). Platelet swirl and pH was not affected by the treatment during 5 days of storage, whereas glucose consumption and lactate production were enhanced as previously described [63].…”

Buffy‐coat platelet variables and metabolism during storage in additive solutions or plasma

“…Unfortunately, regardless of the storage medium, stored PLTs deteriorate, a phenomenon known as the “PLT storage lesion.” This can be characterized by several major changes such as PLT apoptosis and/or fragmentation, which occurs under ambient conditions or under thermostress at 37°C 5 . Also, PLTs in storage tend to release their granular contents, which leads to increased P‐selectin (CD62P) surface expression 10 .…”