Platelet indices as markers of inflammation in systemic sclerosis patients: Relation to vascular endothelial growth factor and flow mediated dilatation

Ibrahim, Saad M.; Morad, Caroline S.; Farouk, Naglaa; Louis, A. Soloff

doi:10.1016/j.ejr.2017.12.001

Cited by 13 publications

(11 citation statements)

References 30 publications

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“…Thrombomodulin and vWF have critical roles in endothelial hemostasis, involved in platelet aggregation and inflammation, further contributing to vasculopathy and fibrosis. 39 , 40 …”

Section: Discussionmentioning

confidence: 99%

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Autologous hematopoietic stem cell transplantation modifies specific aspects of systemic sclerosis-related microvasculopathy

Santana-Gonçalves

Zanin-Silva

Henrique-Neto

et al. 2022

Therapeutic Advances in Musculoskeletal

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Objective: Autologous hematopoietic stem cell transplantation (AHSCT) is a therapeutic option for patients with severe and progressive systemic sclerosis (SSc). Here, we aimed to investigate how AHSCT affects the vasculopathy of SSc patients. Methods: Twenty-seven SSc patients were retrospectively assessed, before and after AHSCT, for vessel morphology (nailfold capillaroscopy), skin expression of endothelial markers and serum levels of markers of inflammation, angiogenesis and endothelial activation. Skin biopsies were analyzed by immunohistochemistry (IHC) for expression of CD31, VE-cadherin, E-selectin, angiopoietin-1 (Ang1), angiopoietin-2 (Ang2), Tie-2, vascular endothelial growth factor A (VEGFA), vascular endothelial growth factor receptor 2 (VEGFR2), and endothelin-1 before and 12 months post-AHSCT. Serum samples from SSc patients were assessed before and up to 36 months after AHSCT for IL-6, von Willebrand factor (vWF), CXC Motif Chemokine Ligand 8 (CXCL8), Endothelin-1, epidermal growth factor (EGF), VEGFA, Pentraxin-3, Intercellular Adhesion Molecule 1 (ICAM-1), E-selectin, P-selectin, Thrombomodulin and IL-18 levels, and compared to healthy control samples. Results: On nailfold capillaroscopy, the number of capillaries increased at 1 year, while giant capillaries decreased at 6 months and 1 year after AHSCT. In the skin biopsies, expression of E-selectin notably decreased and Ang1 increased after AHSCT. At baseline, all vascular markers evaluated in the serum were significantly higher in SSc patients when compared to healthy controls, except for ICAM-1. When compared at different time points after AHSCT, Thrombomodulin, Pentraxin-3, vWF, and IL-18 levels remained generally stable at high levels until 36 months after AHSCT. Conclusion: Our results suggest that AHSCT contributes to improvements of the vessel morphology and dermal microvasculopathy, but does not normalize elevated levels of serum vascular markers in SSc patients. Additional vascular therapeutic approaches might contribute to more effectively treat the endothelial injury.

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“…Thrombomodulin and vWF have critical roles in endothelial hemostasis, involved in platelet aggregation and inflammation, further contributing to vasculopathy and fibrosis. 39 , 40 …”

Section: Discussionmentioning

confidence: 99%

“…Thrombomodulin and vWF have critical roles in endothelial hemostasis, involved in platelet aggregation and inflammation, further contributing to vasculopathy and fibrosis. 39,40 This study is limited by its retrospective design and small sample size. Only 27 of the 39 patients initially evaluated had available samples for analysis.…”

Section: Discussionmentioning

confidence: 99%

Autologous hematopoietic stem cell transplantation modifies specific aspects of systemic sclerosis-related microvasculopathy

Santana-Gonçalves

Zanin-Silva

Henrique-Neto

et al. 2022

Therapeutic Advances in Musculoskeletal

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“…Myofibroblasts may also be originated through the endothelial-to-mesenchymal transition (EndoMT) ( 9 ), when ECs downregulate expression of markers such as CD31 and VE-cadherin, and assume a myofibroblast phenotype, characterized by fusiform morphology and expression of α-SMA ( 10 ). The abnormal vascular tonus and the increased expression of vWF stimulate platelet aggregation and hypercoagulation, leading to further vascular damage ( 11 , 12 ). Reactive oxygen species contribute to further enhance the damage, participating in the initiation and progression of SSc ( 2 , 5 ).…”

Section: Pathophysiology Of the Vascular Endothelium In Systemic Sclerosismentioning

confidence: 99%

Management of Endothelial Dysfunction in Systemic Sclerosis: Current and Developing Strategies

Zanin-Silva

Santana-Gonçalves

Kawashima-Vasconcelos

et al. 2021

Front. Med.

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Systemic Sclerosis (SSc) is an autoimmune disease marked by dysregulation of the immune system, tissue fibrosis and dysfunction of the vasculature. Vascular damage, remodeling and inadequate endothelial repair are hallmarks of the disease. Since early stages of SSc, damage and apoptosis of endothelial cells (ECs) can lead to perivascular inflammation, oxidative stress and tissue hypoxia, resulting in multiple clinical manifestations. Raynaud's phenomenon, edematous puffy hands, digital ulcers, pulmonary artery hypertension, erectile dysfunction, scleroderma renal crisis and heart involvement severely affect quality of life and survival. Understanding pathogenic aspects and biomarkers that reflect endothelial damage in SSc is essential to guide therapeutic interventions. Treatment approaches described for SSc-associated vasculopathy include pharmacological options to improve blood flow and tissue perfusion and, more recently, cellular therapy to enhance endothelial repair, promote angiogenesis and heal injuries. This mini-review examines the current knowledge on cellular and molecular aspects of SSc vasculopathy, as well as established and developing therapeutic approaches for improving the vascular compartment.

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“…Platelet production and its stimulation can be evaluated by measuring parameters, available in complete blood count parameters, like mean platelet volume (MPV), platelet distribution width (PDW), plateletcrit, platelet count. 6 This study aimed to evaluate CBC parameters including platelet indices in patients with arthritis (OA, RA, and gout) and identify them as inflammatory biomarkers.…”

Section: Introductionmentioning

confidence: 99%

Complete Blood Count Parameters in Arthritis

Shrestha

et al. 2020

Nep. Med. Coll. J.

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Osteoarthritis, rheumatoid arthritis, and gout are frequently encountered diseases in the orthopedic clinic. CRP is done to evaluate and monitor these disease processes. This study aimed to evaluate total leukocyte count with differential count, neutrophil/lymphocyte ratio, platelet, platelet/lymphocyte ratio, mean platelet volume (MPV), platelet distribution width (PDW), and plateletcrit in patients with arthritis. This was a cross-sectional study conducted at KIST Medical College, Teaching Hospital from 15 Ashad 2075 to 14 Ashad 2076. The patient’s demographic data and laboratory findings: total leukocyte count, differential count, hemoglobin, platelet count, plateletcrit, mean platelet volume, platelet distribution width, and plateletcrit. CRP, ESR, RA factor/anti-CCP, and Uric acid tests were also performed. Among 67 patients, osteoarthritis was seen in 53 (79.1%), rheumatoid arthritis among 11(16.4%), and Gout among 3(4.5%). The ROC area under the curve was within acceptable limits for the neutrophil count, neutrophil/lymphocyte ratio, mean platelet volume, platelet distribution width. The sensitivity and specificity of the neutrophil count were 75% and 62% respectively (cut-off: 64.5%). Neutrophil/Lymphocyte ratio was increased and correlated with CRP (p-value: <0.001). PDW had a sensitivity of 90% and specificity of 83.0% (cut-off: 16.8 CV). For MPV, the sensitivity was 80% and specificity was 81% (cut-off: 9.1 fL). At 1.92 cut-off value, the sensitivity and specificity of neutrophil/lymphocyte ratio were 80.0% and 62.0% respectively. CBC parameters can provide an important clue to the treating physician, which helps to manage a patient with arthritis effectively. Neutrophil count, Neutrophil/Lymphocyte ratio, MPV, and PDW correlated with other inflammatory biomarkers and can be used to assess the patient with arthritis.

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Platelet indices as markers of inflammation in systemic sclerosis patients: Relation to vascular endothelial growth factor and flow mediated dilatation

Cited by 13 publications

References 30 publications

Autologous hematopoietic stem cell transplantation modifies specific aspects of systemic sclerosis-related microvasculopathy

Autologous hematopoietic stem cell transplantation modifies specific aspects of systemic sclerosis-related microvasculopathy

Management of Endothelial Dysfunction in Systemic Sclerosis: Current and Developing Strategies

Complete Blood Count Parameters in Arthritis

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