Platelet lysate and chondroitin sulfate loaded contact lenses to heal corneal lesions

Sandri, Giuseppina; Bonferoni, Maria Cristina; Delfino, Alessio; Riva, Federica; Cornaglia, Antonia Icaro; Marrubini, Giorgio; Musitelli, G.; Fante, Claudia Del; Perotti, Cesare; Caramella, Carla; Ferrari, Franca

doi:10.1016/j.ijpharm.2016.05.045

Cited by 21 publications

(10 citation statements)

References 19 publications

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“…24 Glycosaminoglycans, such as CS, have been proven to provide biological benefits in corneal tissue engineering, such as promoting corneal wound healing. 19 Therefore, CS was designed to be the backbone of the biocompatible crosslinker by modifying it with NHS, an amine-reactive moiety, which allowed us to apply CS-NHS for collagen fibril crosslinking in animal corneas.…”

Section: Discussionmentioning

confidence: 99%

“…14 Chondroitin sulfate is frequently incorporated into biomaterials with collagen 15 or gelatin 16 to fabricate scaffolds for cartilage or corneal substitutes. 17,18 Contact lenses that had CS loaded into them enhanced healing of corneal lesions, 19 purportedly through collagen fibrillogenesis. In this study, CS was functionally modified with a highly reactive amine group, which was then used in conjunction with NHS to form crosslinks with collagens and proteoglycans present in the cornea.…”

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confidence: 99%

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Chondroitin Sulfate–Based Biocompatible Crosslinker Restores Corneal Mechanics and Collagen Alignment

Wang

Majumdar

et al. 2017

Invest. Ophthalmol. Vis. Sci.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Chondroitin Sulfate–Based Biocompatible Crosslinker Restores Corneal Mechanics and Collagen Alignment

Wang

Majumdar

et al. 2017

Invest. Ophthalmol. Vis. Sci.

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“…Several growth factors (i.e., epidermal growth factor, basic fibroblast growth factor, transforming growth factor-beta, platelet derived growth factor, insulin-like growth factor, and vascular endothelial growth factor) have a role in the control of corneal epithelial cells proliferation, migration, and apoptosis, and they are fundamental in the corneal healing process [203]. The topical administration of growth factors proved to be beneficial in the treatment of persistent epithelial defects, neurotrophic keratitis, and for the recovery of corneal epithelium after surgery [200].…”

Section: Corneal Diseasesmentioning

confidence: 99%

Therapeutic Ophthalmic Lenses: A Review

2020

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An increasing incidence of eye diseases has been registered in the last decades in developed countries due to the ageing of population, changes in lifestyle, environmental factors, and the presence of concomitant medical conditions. The increase of public awareness on ocular conditions leads to an early diagnosis and treatment, as well as an increased demand for more effective and minimally invasive solutions for the treatment of both the anterior and posterior segments of the eye. Despite being the most common route of ophthalmic drug administration, eye drops are associated with compliance issues, drug wastage by lacrimation, and low bioavailability due to the ocular barriers. In order to overcome these problems, the design of drug-eluting ophthalmic lenses constitutes a non-invasive and patient-friendly approach for the sustained drug delivery to the eye. Several examples of therapeutic contact lenses and intraocular lenses have been developed, by means of different strategies of drug loading, leading to promising results. This review aims to report the recent advances in the development of therapeutic ophthalmic lenses for the treatment and/or prophylaxis of eye pathologies (i.e., glaucoma, cataract, corneal diseases, or posterior segment diseases) and it gives an overview of the future perspectives and challenges in the field.

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“…Among the different GFs involved in cardiac repair, VEGF (vascular endothelial growth factor), TGF-b1 (transforming growth factor), and FGF (fibroblast growth factor) have a crucial role in promoting angiogenesis, fibrinogenesis and cardiomyocyte proliferation, respectively [7,20]. Moreover in the current literature, there are several reports of CS effectiveness in enhancing cell proliferation in association with PL, a hemoderivative from platelets rich in GF [21,22,23]. This formulative approach was designed to allow the administration of autologous platelet lysate and to assure a fast translation in clinical practice.…”

Section: Introductionmentioning

confidence: 99%

Electrospun Gelatin–Chondroitin Sulfate Scaffolds Loaded with Platelet Lysate Promote Immature Cardiomyocyte Proliferation

et al. 2018

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Abstract:The aim of the present work was the development of heart patches based on gelatin (G) and chondroitin sulfate (CS) to be used as implants to improve heart recovery after corrective surgery for critical congenital heart defects (CHD). Patches were prepared by means of electrospinning to obtain nanofibrous scaffolds and they were loaded with platelet lysate (PL) as a source of growth factors to further enhance the repair process. Scaffolds were characterized for morphology and mechanical properties and for the capability to support in vitro adhesion and proliferation of dermal fibroblasts in order to assess the system's general biocompatibility. Adhesion and proliferation of endothelial cells and cardiac cells (cardiomyocytes and cardiac fibroblasts from rat fetuses) onto PL-loaded patches was evaluated. Patches presented good elasticity and high stiffness suitable for in vivo adaptation to heart contraction. CS improved adhesion and proliferation of dermal fibroblasts, as proof of their biocompatibility. Moreover, they enhanced the adhesion and proliferation of endothelial cells, a crucial mediator of cardiac repair. Cell adhesion and proliferation could be related to elastic properties, which could favor cell motility. The presence of platelet lysate and CS was crucial for the adhesion and proliferation of cardiac cells and, in particular, of cardiomyocytes: G/CS scaffold embedded with PL appeared to selectively promote proliferation in cardiomyocytes but not cardiac fibroblasts. In conclusion, G/CS scaffold seems to be a promising system to assist myocardial-repair processes in young patient, preserving cardiomyocyte viability and preventing cardiac fibroblast proliferation, likely reducing subsequent uncontrolled collagen deposition by fibroblasts following repair.

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Platelet lysate and chondroitin sulfate loaded contact lenses to heal corneal lesions

Cited by 21 publications

References 19 publications

Chondroitin Sulfate–Based Biocompatible Crosslinker Restores Corneal Mechanics and Collagen Alignment

Chondroitin Sulfate–Based Biocompatible Crosslinker Restores Corneal Mechanics and Collagen Alignment

Therapeutic Ophthalmic Lenses: A Review

Electrospun Gelatin–Chondroitin Sulfate Scaffolds Loaded with Platelet Lysate Promote Immature Cardiomyocyte Proliferation

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