Platelet Mediated Complement Activation

Peerschke, Ellinor I.B.; Ghebrehiwet, Berhane

doi:10.1007/978-0-387-78952-1_7

Cited by 63 publications

(81 citation statements)

References 57 publications

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“…and further deposition of C4 and C3 fragments as well as C5b-C9 complexes (24,39,40). These MP appear to be equivalent in size and AnV expression to the ectosomes described in this work.…”

Section: Discussionsupporting

confidence: 53%

Microparticles (Ectosomes) Shed by Stored Human Platelets Downregulate Macrophages and Modify the Development of Dendritic Cells

Sadallah

Eken

Martin

et al. 2011

The Journal of Immunology

176

158

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Microparticles (MP) shed by platelets (PLT) during storage have procoagulant activities, but little is known about their properties to modify inflammation or immunity. In this study, we studied the capacity of MP present in PLT concentrates to alter the function of macrophages and dendritic cells (DC). The size of the purified MP was between 100 and 1000 nm, and they expressed phosphatidylserine; surface proteins of PLT (CD61, CD36, CD47), including complement inhibitors (CD55, CD59), but not CD63; and proteins acquired from plasma (C1q, C3 fragments, factor H). These characteristics suggest that the MP shed by PLT are formed by budding from the cell surface, corresponding to ectosomes. The purified PLT ectosomes (PLT-Ect) reduced the release of TNF-α and IL-10 by macrophages activated with LPS or zymosan A. In addition, PLT-Ect induced the immediate release of TGF-β from macrophages, a release that was not modified by LPS or zymosan A. Macrophages had a reduced TNF-α release even 24 h after their exposure to PLT-Ect, suggesting that PLT-Ect induced a modification of the differentiation of macrophages. Similarly, the conventional 6-d differentiation of monocytes to immature DC by IL-4 and GM-CSF was modified by the presence of PLT-Ect during the first 2 d. Immature DC expressed less HLA-DP DQ DR and CD80 and lost part of their phagocytic activity, and their LPS-induced maturation was downmodulated when exposed to PLT-Ect. These data indicate that PLT-Ect shed by stored PLT have intrinsic properties that modify macrophage and DC differentiation toward less reactive states.

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“…and further deposition of C4 and C3 fragments as well as C5b-C9 complexes (24,39,40). These MP appear to be equivalent in size and AnV expression to the ectosomes described in this work.…”

Section: Discussionsupporting

confidence: 53%

Microparticles (Ectosomes) Shed by Stored Human Platelets Downregulate Macrophages and Modify the Development of Dendritic Cells

Sadallah

Eken

Martin

et al. 2011

The Journal of Immunology

176

158

View full text Add to dashboard Cite

show abstract

“…Complement activation on platelets, plateletleukocyte complexes, and platelet-derived microparticles may be a mechanism of physiologic clearance of unwanted or apoptotic cells and cell blebs, 26 but it may also activate the cells. 15,27,28 Platelet-and leukocyte-derived microparticles, which were coated with C3 and C9, underwent neutrophil phagocytosis. Complement labeling is a well-studied phenomenon whereby opsonized bacteria undergo phagocytosis by professional phagocytes such as neutrophils, monocytes, macrophages, and, to a lesser extent, dendritic cells, 29 and iC3b is an important opsonin in this process.…”

Section: Discussionmentioning

confidence: 99%

Complement activation on platelet-leukocyte complexes and microparticles in enterohemorrhagic Escherichia coli–induced hemolytic uremic syndrome

Ståhl

Sartz

Karpman

2011

Blood

170

178

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“…Both the coagulation and the complement cascades of the human organism are tightly connected (24). The cross-talk of these two major cascade systems is a focus of ongoing research, and proteases that act in both systems are of special interest.…”

mentioning

confidence: 99%

Plasminogen Is a Complement Inhibitor

Barthel

Schindler

Zipfel

2012

Journal of Biological Chemistry

165

159

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Platelet Mediated Complement Activation

Abstract: The complement system comprises a series of proteases and inhibitors that are activated in cascade-

Cited by 63 publications

References 57 publications

Microparticles (Ectosomes) Shed by Stored Human Platelets Downregulate Macrophages and Modify the Development of Dendritic Cells

Microparticles (Ectosomes) Shed by Stored Human Platelets Downregulate Macrophages and Modify the Development of Dendritic Cells

Complement activation on platelet-leukocyte complexes and microparticles in enterohemorrhagic Escherichia coli–induced hemolytic uremic syndrome

Plasminogen Is a Complement Inhibitor

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