Platelet Phospholipids and Stypven Clotting Time

Abstract

Summary1. Phospholipids in the thrombocytes were studied in 16 healthy individuals, 18 patients with hepatic cirrhosis and 11 patients suffering from a variety of illnesses which are generally considered to be connected with thrombocytopathy.2. In patients with hepatic cirrhosis the total amount of phospholipids was significantly lower: 8 (µg ± 2 µg lipid P per 109 platelets compared to 12 (µg ± 2 (µg lipid P per 109 platelets in healthy individuals.3. The percentage composition of the phospholipids was the same for healthy individuals as for the diseased persons.4. Decrease in platelet factor 3 activity as estimated by a modified stypven clotting time has been demonstrated using the thrombocytes from the patients suffering from hepatic cirrhosis whereas the activity was normal in uremic patients tested. The decreased clotting activity might partly be due to the reduced total amount of phospholipids. Surface active agents exert a much more profound reduction in clotting activity of phospholipids. It is concluded, that surface charge of phosphatide micelles determines their clotting activity more than concentration of all or a single phospholipid.

Serumphospholipide beim sp�t-manifesten Diabetes

“…The aim of the present work, therefore, has been to investigate the total and the individual phospholipids in a selected group of diabetic patients with late manifestations. Since evidence has been obtained that the surface charge of the phospholipids is a most important factor in the process of blood coagulation [10], special emphasis has been given to the determination, not only of the total concentrations of the individual phospholipids, but to the relative distribution, especially of lecithin and cephalins, as separated and quantitated by thin layer chromatography. The patients were selected according to investigations on lipoprotein pattern by paper electrophoresis, and determinations of the concentrations of total cholesterol and triglycerides.…”

Effects of Some Polyene Antibiotics on Human Platelets and their Function

“…repeated freezing and thawing or incubation with particles such as kaolin [11][12][13]23], The release of PF3 in the presence of polyenes was in vestigated using the method of Spaet and Cintron [23], modified appropri ately. Polyenes were added to platelet-rich plasmas with varying platelet count and the clotting times recorded.…”