Platelet-Rich fibrin: A second generation platelet concentrate and a new friend of oral and maxillofacial surgeons

Abstract

To assess the potential use and benefits of Platelet-Rich Fibrin (PRF) over Platelet-Rich Plasma (PRP), for wound healing post oral and maxillofacial surgeries. This article describes the evolution of this second generation platelet concentrate and its multiple uses in various surgical procedures. Around 5 ml of whole venous blood is collected from the patients in each of the two sterile vacutainer tubes of 6 ml capacity without anticoagulant. The vacutainer tubes are then placed in a centrifugal machine at 3000 revolutions per minute (rpm) for 10 minutes, and the middle fraction containing the fibrin clot is then collected 2 mm below lower dividing line, to obtain the PRF. Cavities filled with PRF post oral and maxillofacial surgical procedures, at the institute, showed faster healing in half the time as compared to physiologic healing. PRF, which belongs to a new second generation of platelet concentrates, with simplified processing, and not requiring biochemical blood handling, has several advantages over traditionally prepared PRP, which has been widely used for accelerating soft tissue and hard tissue healing. However, the preparation being strictly autologous, the amount of PRF obtained is limited.

Platelet-Rich Fibrin as a New Approach in Treating Gingival Recession: Systematic Review and Meta-Analysis

“…This finding is explained by PRF characteristics, when compared to other forms of blood derived growth factors like PRP, in the slow release of growth factors over a period of 10 days which is the time needed for revascularization and CT formation in a soft tissue regeneration procedure, while PRP was known for an earlier release of growth factors in the healing cascade [53][54][55]. A meticulous care should be taken in terms of proper handling of PRF, as well as immediate application to recession site after preparation [56,57]. It should be noted that some of the drawbacks for the use of PRF in root coverage procedure is the need to cover the membrane completely to prevent its early resorption, which requires clinical case pre-requisite for a successful coronally advanced flap including; the presence of keratinized tissues, a recession depth not exceeding 4 mm, the presence of a vestibular depth to prevent flap tension, the placement of final flap margin coronal to CEJ, and a tissue biotype thickness of no less than 0.8 mm [58,59].…”

Mesenchymal Stem Cells and Platelet Derived Concentrates in Regenerative Medicine

“…PRF (Figure 3), a second-generation platelet-derived concentrate, is made by a centrifugation-based method, and there is no chemical material use for PRF preparation [41].…”

“…osteochondral lesions, synovial knee joint failure, acute diaphyseal fractures of the femur, trauma, maxillofacial defects, periodontal tissue augmentation, peripheral nerve injuries, chondral lesions of the hip, wound, degenerative disc disease, femoral neck fractures, oral peri-implant defects, and hair loss [28][29][30][31][32][33][34][35][36][37][38][39][40][41][42].…”

Oroantral Communication Repair Using Platelet-Rich Fibrin

“…[18][19][20][21][22] Platelet-rich fibrin (PRF) is defined as an autogenous solid biomaterial, containing increased amount of leukocytes and platelets. [23,24] PRF is used as a stimulating factor of the bone and soft tissue regeneration in dental implantology and periodontal surgery. [25] It is used for the healing of extraction wounds, [26] treatment of interosseous defects, [27] radicular cysts, [28] influencing the jaw bones in the case of biophosphonate osteonecrosis, [29] etc.…”

“…[25] It is used for the healing of extraction wounds, [26] treatment of interosseous defects, [27] radicular cysts, [28] influencing the jaw bones in the case of biophosphonate osteonecrosis, [29] etc. Based on the literature data [30][31][32][33] and our clinical experience with the biopotential of PRF [34][35][36] we set out to test the possibilities of using PRF in the treatment of maxillary sinus perforations.…”