Platelet-rich Fibrin Increases Proliferation and Differentiation of Human Dental Pulp Cells

Wound healing is a staged process which involves the activity of leukocytes and platelets. For this process to work efficiently, the platelets play a vital role. The growth factors present in platelets are important to guide the regenerating cells to the area of healing. Platelet-rich-fibrin (PRF) is one such material that holds on to these growth factors enmeshed in the fibrin network resulting in their sustained release over a period of time that can accelerate the wound healing process. With this knowledge, research has been carried out for a past few years for the clinical application of PRF. Various platelet concentrates have been studied including the platelet-rich-plasma (PRP). However, the short duration of cytokine release and its poor mechanical properties have resulted in the search of a new material with adequate properties for clinical application and ease of preparation. PRF has found a place in the regenerative field owing to its advantages over PRP. This review focuses on the properties and various applications of PRF in the clinical practice.

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“…(1) PRF promotes dentinogenesis by stimulating cell proliferation and differentiation of Dental Pulp Cells [35].…”

Section: Extraoral Clinical Applicationsmentioning

confidence: 99%

Platelet-Rich Fibrin as a Biofuel for Tissue Regeneration

Khiste

Tari

2013

ISRN Biomaterials

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“…Another advantage is the release of bioactive molecules, which seems to be more efficient in fibrin concentrates. Platelet compounds have several bioactive molecules capable of stimulating cell migration, differentiation and proliferation 48 . In this way, blood plasma concentrates, in addition to acting as excellent scaffolds for cell adhesion, are naturally loaded with growth factors, which does not occur with synthetic scaffolds, which need to be loaded individually 15 .…”

Section: Discussionmentioning

confidence: 99%

What is the clinical applicability of regenerative therapies in dentistry?

Demarco

Kirschnick

Watson

et al. 2017

RGO, Rev. Gaúch. Odontol.

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Regenerative therapies have been widely developed in dentistry and it is important to incorporate dentists' knowledge of these new therapies RESUMOTerapias regenerativas vem sendo amplamente desenvolvidas na odontologia e o conhecimento destas novas terapias por parte dos dentistas é importante para que elas sejam incorporadas na rotina clínica odontológica. Assim, este estudo revisou a literatura acerca das terapias regenerativas e suas aplicações clínicas. A engenharia tecidual tem contribuído na mudança do paradigma restaurador das ciências da saúde. Seus pilares embasam as técnicas de regeneração de tecidos e órgãos. Apesar da grande maioria dos estudos neste campo ser in vitro, uma gama de metodologias pré-clínicas foi consolidada e estudos utilizando estes princípios já estão sendo empregados em humanos. A utilização de plasma rico em plaquetas e plasma rico em fibrina como scaffolds naturais em cirurgias para reestabelecimento de tecido ósseo e periodontal são frequentemente relatadas na literatura e ensaios clínicos utilizando esta abordagem demonstram resultados promissores. Células-tronco da polpa dental autólogas já foram aplicadas com sucesso na regeneração de tecido ósseo utilizando scaffold naturais de colágeno em humanos. Além disto, a revascularização do canal radicular já aparece na literatura como uma alternativa promissora frente a opção de apecificação do canal radicular. Esta terapia utiliza como princípio o coágulo sanguíneo como scaffold e a migração das células-tronco da papila apical para regenerar o órgão pulpar. Considerações finais: Apesar de incipientes, as terapias regenerativas já podem ser utilizadas na prática clínica odontológica. O conhecimento dos princípios que regem estas terapias deve ser compreendido pelo dentista para que sejam utilizadas na prática clínica.Termos de indexação: Fibrina. Plasma rico em plaquetas. Células-tronco. Engenharia tecidual.

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“…The intrinsic incorporation of cytokines within the fibrin mesh allows for their progressive release over time (7-10 days), as the network of fibrin disintegrates [23]. Stimulated secretion of osteoprotegrin and indirect inhibition of RANKL RANK system may be responsible for osteoblastogenesis [24]. There is a slow sustained release of growth factors over time [25].…”

Section: Discussionmentioning

confidence: 99%