Platelet-rich plasma accelerates skin wound healing by promoting re-epithelialization

Abstract

Background Autologous platelet-rich plasma (PRP) has been suggested to be effective for wound healing. However, evidence for its use in patients with acute and chronic wounds remains insufficient. The aims of this study were to comprehensively examine the effectiveness, synergy and possible mechanism of PRP-mediated improvement of acute skin wound repair. Methods Full-thickness wounds were made on the back of C57/BL6 mice. PRP or saline solution as a control was administered to the wound area. Wound healing rate, local inflammation, angiogenesis, re-epithelialization and collagen deposition were measured at days 3, 5, 7 and 14 after skin injury. The biological character of epidermal stem cells (ESCs), which reflect the potential for re-epithelialization, was further evaluated in vitro and in vivo. Results PRP strongly improved skin wound healing, which was associated with regulation of local inflammation, enhancement of angiogenesis and re-epithelialization. PRP treatment significantly reduced the production of inflammatory cytokines interleukin-17A and interleukin-1β. An increase in the local vessel intensity and enhancement of re-epithelialization were also observed in animals with PRP administration and were associated with enhanced secretion of growth factors such as vascular endothelial growth factor and insulin-like growth factor-1. Moreover, PRP treatment ameliorated the survival and activated the migration and proliferation of primary cultured ESCs, and these effects were accompanied by the differentiation of ESCs into adult cells following the changes of CD49f and keratin 10 and keratin 14. Conclusion PRP improved skin wound healing by modulating inflammation and increasing angiogenesis and re-epithelialization. However, the underlying regulatory mechanism needs to be investigated in the future. Our data provide a preliminary theoretical foundation for the clinical administration of PRP in wound healing and skin regeneration.

Recombinant human epidermal growth factor combined with vacuum sealing drainage for wound healing in Bama pigs

“…The wound healing process is a complex biological event that includes inflammation and the proliferation and migration of different cell types, and its aim is to restore the physiological and barrier functions of the skin, and then control infection [ 3 ]. At the same time, there are several important and orderly physiological events in the healing process, such as extracellular matrix (ECM) synthesis, neovascularization [ 4 ], and collagen secretion and deposition leading to re-epithelialization and the formation of granulation tissue [ 5 ].…”

Adipose tissue micrograft in a scaffold of plasma-gel combined with platelet-derived growth factors in dermal wrinkle regeneration

“…10 Therefore, a portion of autologous plasma for PRP treatments is not used. 11 This study involved the use of a viable micrograft of adipose tissue containing progenitors in suspension, with markers of adult stem cell on the surface (CD) from 2 mL of subcutaneous fat, which was taken and disrupted as indicated by Tonnard 2013, then microfiltered through a 50-micron filter combined with a plasma gel scaffold (STBA), suitable for use as an autologous biological tissue support since it is not inflammatory. [5][6][7][13][14][15] It is also envisaged the union of the buffy coat with the suspension obtained immediately before, order allow the physiological activation of progenitors through the surface CDs.…”

Platelet-Rich Plasma Promotes the Proliferation of Human Keratinocytes via a Progression of the Cell Cycle. A Role of Prolidase

“…Notably, platelets contain more than 300 biologically active molecules that are released upon activation from α-granules and dense granules of platelets and subsequently may regulate the tissue regeneration process [ 2 , 3 , 4 ]. Activated thrombocytes are a source of multiple growth factors (GFs) and cytokines, including platelet-derived growth factor (PDGF), basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF), insulin-like growth factor-1 (IGF-1), transforming growth factor-β (TGF-β), and others [ 5 , 6 ]. GFs are involved in specific biomolecular functions during tissue repair playing a fundamental role in wound healing.…”

“…Some findings suggest that PRP has a strong effect on vascularization. PRP contains VEGF that promotes the formation of vessels in burn wounds [ 5 , 9 ]. Therefore, PRP is used in clinical treatment of acute and chronic wounds with good results.…”

“…Therefore, PRP is used in clinical treatment of acute and chronic wounds with good results. PRP can also increase the healing rate of diabetic wounds, reduce the injured area of ulcers, and improve joint function in osteoarthritis [ 5 , 10 , 11 , 12 ]. Recent studies suggest that prolidase could play an important role in the tissue repair process [ 13 ].…”