Platelet-rich plasma affects the proliferation of canine bone marrow-derived mesenchymal stromal cells in vitro

Pandey, Sony; Hickey, Dawn U.; Drum, Marti G.; Millis, Darryl L.; Cekanova, Maria

doi:10.1186/s12917-019-2010-x

Cited by 8 publications

(13 citation statements)

References 37 publications

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“…In the following treatment period, the syringes with the medium of one array were switched to medium containing 10% human blood serum. In vitro cultivation of adult human stem cells with human blood plasma or serum has demonstrated increased proliferation and viability [ 14 , 15 , 16 , 17 ]. To investigate a potential effect of human serum on hCSC migration, we tracked the covered path of single cells that were cultivated under exposure to blood serum or to control medium in our microfluidic device.…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, the use of convalescent plasma is currently under discussion as a treatment option in the COVID-19 pandemic [ 12 , 13 ]. In vitro cultivation of adult human stem cells with human blood plasma or serum has demonstrated increased proliferation and viability [ 14 , 15 , 16 , 17 ]. Mishima and coworkers showed increased migration of human articular chondrocytes and mesenchymal stem cells (MSCs) in response to 5, 10 and 20% fetal bovine serum (FBS) [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

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Human Blood Serum Induces p38-MAPK- and Hsp27-Dependent Migration Dynamics of Adult Human Cardiac Stem Cells: Single-Cell Analysis via a Microfluidic-Based Cultivation Platform

Höving

Schmitz

Schmidt

et al. 2021

Biology

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Migratory capabilities of adult human stem cells are vital for assuring endogenous tissue regeneration and stem cell-based clinical applications. Although human blood serum has been shown to be beneficial for cell migration and proliferation, little is known about its impact on the migratory behavior of cardiac stem cells and underlying signaling pathways. Within this study, we investigated the effects of human blood serum on primary human cardiac stem cells (hCSCs) from the adult heart auricle. On a technical level, we took advantage of a microfluidic cultivation platform, which allowed us to characterize cell morphologies and track migration of single hCSCs via live cell imaging over a period of up to 48 h. Our findings showed a significantly increased migration distance and speed of hCSCs after treatment with human serum compared to control. Exposure of blood serum-stimulated hCSCs to the p38 mitogen-activated protein kinase (p38-MAPK) inhibitor SB239063 resulted in significantly decreased migration. Moreover, we revealed increased phosphorylation of heat shock protein 27 (Hsp27) upon serum treatment, which was diminished by p38-MAPK-inhibition. In summary, we demonstrate human blood serum as a strong inducer of adult human cardiac stem cell migration dependent on p38-MAPK/Hsp27-signalling. Our findings further emphasize the great potential of microfluidic cultivation devices for assessing spatio-temporal migration dynamics of adult human stem cells on a single-cell level.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Human Blood Serum Induces p38-MAPK- and Hsp27-Dependent Migration Dynamics of Adult Human Cardiac Stem Cells: Single-Cell Analysis via a Microfluidic-Based Cultivation Platform

Höving

Schmitz

Schmidt

et al. 2021

Biology

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show abstract

“…Isolated hCSCs were able to grow clonally while maintaining their capability of sphere formation and differentiated efficiently into α-actinin + cardiomyocyte-like cells. Although the application of human blood serum, blood plasma or platelet-rich plasma on stem cell cultures has already been shown to effectively promote cell proliferation [21,22,[49][50][51][52][53], the cardiovascular system and especially cardiac stem cells have not been investigated so far. Within this study we likewise could demonstrate an overall enhancing effect of human blood serum on adult human cardiac stem cells, while no age dependency could be detected in terms of proliferation and metabolic activity.…”

Section: Discussionmentioning

confidence: 99%

Blood Serum Stimulates p38-Mediated Proliferation and Changes in Global Gene Expression of Adult Human Cardiac Stem Cells

Höving

Schmidt

Merten

et al. 2020

Cells

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During aging, senescent cells accumulate in various tissues accompanied by decreased regenerative capacities of quiescent stem cells, resulting in deteriorated organ function and overall degeneration. In this regard, the adult human heart with a generally low regenerative potential is of extreme interest as a target for rejuvenating strategies with blood borne factors that might be able to activate endogenous stem cell populations. Here, we investigated for the first time the effects of human blood plasma and serum on adult human cardiac stem cells (hCSCs) and showed significantly increased proliferation capacities and metabolism accompanied by a significant decrease of senescent cells, demonstrating a beneficial serum-mediated effect that seemed to be independent of age and sex. However, RNA-seq analysis of serum-treated hCSCs revealed profound effects on gene expression depending on the age and sex of the plasma donor. We further successfully identified key pathways that are affected by serum treatment with p38-MAPK playing a regulatory role in protection from senescence and in the promotion of proliferation in a serum-dependent manner. Inhibition of p38-MAPK resulted in a decline of these serum-mediated beneficial effects on hCSCs in terms of decreased proliferation and accelerated senescence. In summary, we provide new insights in the regulatory networks behind serum-mediated protective effects on adult human cardiac stem cells.

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“…The osteogenic experiments showed that PRP induced significantly greater mineralization of hDPCs. Some recent studies have developed culture conditions for in vitro expansion of cells treated by PRP ( Lucarelli et al, 2003 ; Yeom et al, 2016 ; Pandey et al, 2019 ; Hahn et al, 2020 ). These research articles also focused on optimizing concentrations of PRP used for treatments ( Lucarelli et al, 2003 ; Pandey et al, 2019 ; Wang et al, 2019 ; Hahn et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…Some recent studies have developed culture conditions for in vitro expansion of cells treated by PRP ( Lucarelli et al, 2003 ; Yeom et al, 2016 ; Pandey et al, 2019 ; Hahn et al, 2020 ). These research articles also focused on optimizing concentrations of PRP used for treatments ( Lucarelli et al, 2003 ; Pandey et al, 2019 ; Wang et al, 2019 ; Hahn et al, 2020 ). Pandey et al reported that 2.5–20% PRP (v/v) concentrations promoted cell migration and proliferation in vitro , while 40% PRP suppressed their migration and proliferation ( Pandey et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Platelet-Rich Plasma Induces Autophagy and Promotes Regeneration in Human Dental Pulp Cells

Zhao

et al. 2021

Front. Bioeng. Biotechnol.

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Regenerative endodontic procedures using autologous platelet-rich plasma (PRP) can improve the biologic outcome of treatment. However, its mechanism of action on improving pulp regeneration is not fully elucidated. Autophagy was recently shown to be related to tissue repair and osteogenesis. Therefore, the objective of this study was to investigate the effect of PRP in dental pulp regeneration and to elucidate the role of autophagy involved in this process. Human dental pulp cells (hDPCs) were isolated from healthy dental pulp and co-cultured with an increasing concentration of PRP. Cellular migration and proliferation were determined by scratch assay, transwell assay, and cell-counting kit 8 assay. Osteogenic differentiation was clarified by using alkaline phosphatase staining, alizarin red staining, and real-time polymerase chain reaction (RT-PCR) to measure the gene expression levels of alkaline phosphatase, collagen-1, osteocalcin, dentin matrix protein 1, and dentin sialophosphoprotein. Autophagic bodies were observed by transmission electron microscopy and the expression of autophagy marker light chain 3B (LC3B) was determined by immunofluorescence staining. The mRNA and protein expression level of LC3B and Beclin-1 were quantified by qRT-PCR and western blotting. The effect of PRP on cellular migration, proliferation, and osteogenic differentiation was further investigated in the milieu of autophagy activator, rapamycin, and inhibitor, 3-methyladenine. Results showed that PRP promoted cell migration, proliferation, and osteogenic differentiation. Autophagic bodies were strongly activated and the expression level of LC3B and Beclin-1 was significantly promoted by PRP. Autophagy inhibition suppressed PRP-induced hDPCs migration, proliferation, and osteogenic differentiation, whereas autophagy activator substantially augmented PRP-stimulated migration, proliferation, and differentiation. Taken together, these findings suggested that PRP could effectively promote regenerative potentials associated with autophagy.

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Platelet-rich plasma affects the proliferation of canine bone marrow-derived mesenchymal stromal cells in vitro

Cited by 8 publications

References 37 publications

Human Blood Serum Induces p38-MAPK- and Hsp27-Dependent Migration Dynamics of Adult Human Cardiac Stem Cells: Single-Cell Analysis via a Microfluidic-Based Cultivation Platform

Human Blood Serum Induces p38-MAPK- and Hsp27-Dependent Migration Dynamics of Adult Human Cardiac Stem Cells: Single-Cell Analysis via a Microfluidic-Based Cultivation Platform

Blood Serum Stimulates p38-Mediated Proliferation and Changes in Global Gene Expression of Adult Human Cardiac Stem Cells

Platelet-Rich Plasma Induces Autophagy and Promotes Regeneration in Human Dental Pulp Cells

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