Platelet-Rich Plasma Attenuates 30-kDa Fibronectin Fragment–Induced Chemokine and Matrix Metalloproteinase Expression by Meniscocytes and Articular Chondrocytes

Wang, Chih Chien; Lee, Chian Her; Peng, Yi‐Jen; Salter, Donald; Lee, Herng Sheng

doi:10.1177/0363546515597489

Cited by 21 publications

(16 citation statements)

References 59 publications

(68 reference statements)

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“…We considered that the superior healing process and chondroprotective phenomenon that occurred in the PRF-augmented suture group were attributable not only to the anabolic cytokines but also the presence of a number of anti-inflammatory mediators that can potentially affect numerous overlapping pathways simultaneously [ 46 ]. For instance, PRP was found effective in attenuating the effects of fibronectin fragments on both chondrocyte and meniscocytes production of procatabolic chemokines and matrix metalloproteinases [ 47 ]. In the future, the role of PRF in modulating inflammatory mediators warrants further in vitro and in vivo studies using large animals to further confirm the potential of clinical translation while exploring the underlying mechanisms.…”

Section: Discussionmentioning

confidence: 99%

Platelet-Rich Fibrin Facilitates Rabbit Meniscal Repair by Promoting Meniscocytes Proliferation, Migration, and Extracellular Matrix Synthesis

Wong

Kuo

Yang

et al. 2017

IJMS

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Although platelet-rich fibrin (PRF) has been used in clinical practice for some time, to date, few studies reveal its role as a bioactive scaffold in facilitating meniscal repair. Here, the positive anabolic effects of PRF on meniscocytes harvested from the primary culture of a rabbit meniscus were revealed. The rabbit meniscocytes were cultured with different concentrations of PRF-conditioned medium, and were evaluated for their ability to stimulate cell migration, proliferation, and extracellular matrix formation. In vivo, meniscal defects were created via an established rabbit animal model and were evaluated by a histology-based four-stage scoring system to validate the treatment outcome three months postoperatively. The in vitro results showed that PRF could induce cellular migration and promote proliferation and meniscocyte extracellular matrix (ECM) synthesis of cultured meniscocytes. In addition, PRF increased the formation and deposition of cartilaginous matrix produced by cultured meniscocytes. Morphological and histological evaluations demonstrated that PRF could facilitate rabbit meniscal repair. The data highlight the potential utility of using PRF in augmenting the healing of meniscal injuries. These advantages would benefit clinical translation, and are a potential new treatment strategy for meniscal repair.

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Section: Discussionmentioning

confidence: 99%

Platelet-Rich Fibrin Facilitates Rabbit Meniscal Repair by Promoting Meniscocytes Proliferation, Migration, and Extracellular Matrix Synthesis

Wong

Kuo

Yang

et al. 2017

IJMS

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“…Inflammation, wound healing, and regeneration PRP has been shown to attenuate fibronectin-induced expression of several pro-inflammatory chemokines and MMPs in human meniscocytes and articular chondrocytes, thus supporting use of PRP in the management of cartilage and meniscal injuries [64]. It was also proposed as a potential treatment for endometritis in cattle based on its abilities to downregulate the expression of pro-inflammatory genes in primary cultures of bovine endometrial cells after 48 h of incubation [65], and decreased intrauterine inflammatory response in mares [66].…”

Section: Prp Stimulates Endometrial Cell Proliferation and Migrationmentioning

confidence: 93%

In vitro evidence that platelet-rich plasma stimulates cellular processes involved in endometrial regeneration

Aghajanova

Houshdaran

Balayan

et al. 2018

J Assist Reprod Genet

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“…PRP represents a safe and cheaper alternative to recombinant growth factors, releasing an autologous and appropriate cocktail of growth factors within a natural and physiological range at the site of injection. In vitro, it has been shown that PRP has a proliferative effect on autologous chondrocytes and mesenchymal stem cells [53,54] and attenuates their pro-inflammatory chemokine and metalloproteinase expression [55]. Clinical studies evaluating the efficacy of PRP, prepared with Regen Lab technology, for the treatment of knee osteoarthritis demonstrated significant effects on pain relief and functional improvement, especially for those patients with lower degrees of OA, with safety of use and no serious adverse events attributable to the treatment [8,[56][57][58][59][60][61][62].…”

Section: Platelet Rich Plasma In the Treatment Of Osteoarthritismentioning

confidence: 99%

Technology rationale for the development of CellularMatrix® A-CP-HA Kit, certified medical device allowing the combination of platelet rich plasma and hyaluronic acid for the treatment of osteoarthritis

Vischer¹,

Fourmestraux²,

Turzi³

2018

nt. J. Clin. Rheumatol

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International Journal of Clinical Rheumatology Opinion Article Technology rationale for the development of CellularMatrix® A-CP-HA Kit, certified medical device allowing the combination of platelet rich plasma and hyaluronic acid for the treatment of osteoarthritis The composition of synovial fluid of patients suffering from osteoarthritis (OA) is altered with reduced concentration and molecular weight of Hyaluronic Acid (HA) and augmentation of catabolic enzymes and inflammatory markers. Intra-Articular (IA) injections of exogenous HA aim to restore the rheological properties of the synovial fluid in the osteoarthritic joints. However, clinical studies have shown that the benefit of HA injections lasts only for around 6 months and that many patients don't respond well to repeated course of HA treatment. On the other side, platelet rich plasma (PRP) IA injections have been shown to reduce pain and improve joint mobility, probably by modulating the expression of catabolic enzymes and inflammatory markers. As injections of HA and PRP use different pathway to alleviate symptoms in OA patients, the concept of combining these two treatments has emerged recently. In vitro evaluations and preliminary studies have demonstrated the therapeutic potential of this new therapeutic approach for OA treatment. To meet the needs of medical practitioners that want to treat their patients with this new treatment option, an innovative medical device, the CellularMatrix A-CP-HA Kit, has been specifically designed. This device is the first certified medical device that allows the combination of PRP and HA in manner compliant with medical devices regulation and good clinical practice.

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Platelet-Rich Plasma Attenuates 30-kDa Fibronectin Fragment–Induced Chemokine and Matrix Metalloproteinase Expression by Meniscocytes and Articular Chondrocytes

Abstract: These observations provide support for the use and further trials of PRP in management of cartilage and meniscal injuries.

Cited by 21 publications

References 59 publications

Platelet-Rich Fibrin Facilitates Rabbit Meniscal Repair by Promoting Meniscocytes Proliferation, Migration, and Extracellular Matrix Synthesis

Platelet-Rich Fibrin Facilitates Rabbit Meniscal Repair by Promoting Meniscocytes Proliferation, Migration, and Extracellular Matrix Synthesis

In vitro evidence that platelet-rich plasma stimulates cellular processes involved in endometrial regeneration

Technology rationale for the development of CellularMatrix® A-CP-HA Kit, certified medical device allowing the combination of platelet rich plasma and hyaluronic acid for the treatment of osteoarthritis

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