Platelet-rich plasma-containing fragmin-protamine micro-nanoparticles promote epithelialization and angiogenesis in split-thickness skin graft donor sites

Takabayashi, Yuki; Sumi, Yuki; Takikawa, Makoto; Nakamura, Shingo; Kiyosawa, Tomoharu

doi:10.1016/j.jss.2014.08.011

Cited by 21 publications

(10 citation statements)

References 25 publications

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“…The barrier functions of skin tissue mainly embodies in two aspects: on one hand, it is to prevent the internal milieu disorder caused by the loss of internal water in the body, electrolyte and other substances; on the other hand, it is to prevent the body from invasion of various toxic substances (Roche-Kubler et al, 2015;Takabayashi et al, 2015). Many factors, such as large area deep burn, trauma, chronic ulcer, and some operations including congenital malformation operation and tumor resection often cause skin defects, which needs adequate skin to cover the wound to promote wound repair (Raza et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Effects of intravenous arginine on the healing of human skin graft donor sites: A randomized controlled trial

Lin

et al. 2015

Bangladesh J Pharmacol

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We expounded the effect of intravenous arginine on the healing of human skin graft donor sites. This randomized study was double blind and controlled by placebos from October 2012 to May 2015. Nine patients were selected into the arginine group and 10 were selected into the control group. There was no significance in age, weight, BMI, CRP, albumin and total plasma protein in arginine and control groups. Angiogenesis, re-epithelialization and neutrophil influx of wound healing were measured. The concentrations of plasma amino acid were measured to evaluate our intervention. In our study, plasma arginine and ornithine concentrations in arginine group were the highest after arginine-treated at 2 days. Supplementation of intravenous arginine could not affect on healing of human skin graft donor sites, improve angiogenesis, reepithelialization or neutrophil influx in rats. Article Info

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Section: Introductionmentioning

confidence: 99%

Effects of intravenous arginine on the healing of human skin graft donor sites: A randomized controlled trial

Lin

et al. 2015

Bangladesh J Pharmacol

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“…The use of concentrated platelet solutions or platelet lysate in soft tissue wound healing has been investigated . Although PRP has been studied in oral maxillofacial, orthopedic, and plastic surgical applications, there remains conflicting evidence and a glaring lack of consensus regarding its clinical benefit .…”

Section: Discussionmentioning

confidence: 99%

“…The use of concentrated platelet solutions or platelet lysate in soft tissue wound healing has been investigated. 8,11,13,23 Although PRP has been studied in oral maxillofacial, orthopedic, and plastic surgical applications, there remains conflicting evidence and a glaring lack of consensus regarding its clinical benefit. 8,10,[24][25][26][27][28][29] This is due in large part to the lack of standardized dosing and improper biologic characterization of PRP, and the literature is primarily composed of small case series, anecdotal case reports and inappropriately powered clinical trials.…”

Section: Discussionmentioning

confidence: 99%

Cross‐linking of porcine acellular dermal matrices negatively affects induced neovessel formation using platelet‐rich plasma in a rat model of hernia repair

Fernandez-Moure

Eps

Peterson

et al. 2017

Wound Repair Regeneration

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The degree of cross-linking within acellular dermal matrices (ADM) seems to correlate to neovascularization when used in ventral hernia repair (VHR). Platelet-rich plasma (PRP) enhances wound healing through several mechanisms including neovascularization, but research regarding its effect on soft tissue healing in VHR is lacking. We sought to study the effect of cross-linking on PRP-induced neovascularization in a rodent model of bridging VHR. We hypothesized that ADM cross-linking would negatively affect PRP-induced neovessel formation. PRP was extracted and characterized from pooled whole blood. Porcine cross-linked (cADM) and non-cross-linked ADMs (ncADM) were implanted in a rat model of chronic VHR after treatment with saline (control) or PRP. Neovascularization of samples at 2, 4, and 6 weeks was assessed by hematoxylin and eosin and immunohistochemical staining of CD 31. Adhesion severity at necropsy was compared using a previously validated scale. Addition of PRP increased neovascularization in both cADM and ncADM at 2- and 4-week time points but appeared to do so in a dependent fashion, with significantly greater neovascularization in the PRP-treated ncADMs compared to cADMs. Omental adhesions were increased in all PRP-treated groups. Results indicate that, for 2-week measurements when compared with the cADM group without PRP therapy, the mean change in neovascularization due to ncADM was 3.27 (Z = 2.75, p = 0.006), PRP was 17.56 (Z = 14.77, p < 0.001), and the combined effect of ncADM and PRP was 9.41 (Z = 5.6, p < 0.001). The 4-week data indicate that the average neovascularization change due to ncADM was 0.676 (Z = 0.7, p = 0.484), PRP was 7.69 (Z = 7.95, p < 0.001), and combined effect of ncADM and PRP was 5.28 (Z = 3.86, p < 0.001). These findings validate PRP as a clinical adjunct to enhance the native tissue response to implantable biomaterials and suggest that ncADM is more amenable than cADM to induced neovascularization. PRP use could be advantageous in patients undergoing VHR where poor incorporation is anticipated and early-enhanced neovascularization is desired.

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“…Many studies on humans showed no reduction in the density of skin capillaries. This result implies that microvascular function might be so abnormal that it reduces blood flow to the tissue even though there is no reduction in overall vascular density in patients suffering from diabetes [11,12]. Recent studies on diabetic animals revealed a reduction in Vascular Endothelial Growth Factor (VEGF) which is known to promote angiogenesis and a tropical application of VEGF improved would healing [13,14].…”

Section: Discussionmentioning

confidence: 99%

Survival of Split Thickness Skin Graft in Diabetic and Non-diabetic Wound Management

Maramreddy¹

2016

IJARS

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Introduction: Split skin grafting is widely used surgical procedure for the treatment of ulcers. Graft survival depends on number of factors like vascularity, wound infection etc. diabetes is associated with endothelial dysfunction, neuropathy, wound infection which collectively affect the graft survival. Aim:To compare the amount of graft uptake, the post operative complications and survival of split thickness skin graft in diabetic and non diabetic wound management. Materials and Methods:In our prospective nonrandomized comparative study total 104 patients with ulcer were included of which 52 were diabetic and 52 were non-diabetic. All of them underwent split skin grafting as part of their wound management. Comparison was made between two groups in terms of amount of graft uptake, post operative wound infection, re-ulceration, revisional surgery, donor site infection.

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Platelet-rich plasma-containing fragmin-protamine micro-nanoparticles promote epithelialization and angiogenesis in split-thickness skin graft donor sites

Cited by 21 publications

References 25 publications

Effects of intravenous arginine on the healing of human skin graft donor sites: A randomized controlled trial

Effects of intravenous arginine on the healing of human skin graft donor sites: A randomized controlled trial

Cross‐linking of porcine acellular dermal matrices negatively affects induced neovessel formation using platelet‐rich plasma in a rat model of hernia repair

Survival of Split Thickness Skin Graft in Diabetic and Non-diabetic Wound Management

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