Platelet-Rich Plasma Derived Growth Factors Contribute to Stem Cell Differentiation in Musculoskeletal Regeneration

Qian, Yun; Han, Qixin; Chen, Wei; Song, Jialin; Zhao, Xiaotian; Ouyang, Yuanming; Yuan, Weien; Fan, Cunyi

doi:10.3389/fchem.2017.00089

Cited by 118 publications

(91 citation statements)

References 71 publications

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“…Several previously confirmed chondro-inductive stimuli of platelet concentrates to MSCs [38,[53][54][55], whereas others indicate that PRP treatment does not improve the in vitro chondrogenesis of MSCs [56]. The difference between the previously identified differentiation-promoting effects of platelets aggregates such as PRP in musculoskeletal diseases (reviewed by Qian et al [57]), and our data on L-PRF might be caused by different factors. First, various platelet concentrates have different release kinetics [58].…”

Section: Discussionsupporting

confidence: 54%

Therapeutic Potential of Dental Pulp Stem Cells and Leukocyte- and Platelet-Rich Fibrin for Osteoarthritis

Monaco

Gervois

Beaumont

et al. 2020

Cells

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Osteoarthritis (OA) is a degenerative and inflammatory joint disorder with cartilage loss. Dental pulp stem cells (DPSCs) can undergo chondrogenic differentiation and secrete growth factors associated with tissue repair and immunomodulation. Leukocyte-and platelet-rich fibrin (L-PRF) emerges in regenerative medicine because of its growth factor content and fibrin matrix. This study evaluates the therapeutic application of DPSCs and L-PRF in OA via immunomodulation and cartilage regeneration. Chondrogenic differentiation of DPSCs, with or without L-PRF exudate (ex) and conditioned medium (CM), and of bone marrow-mesenchymal stem cells was compared. These cells showed differential chondrogenesis. L-PRF was unable to increase cartilage-associated components. Immature murine articular chondrocytes (iMACs) were cultured with L-PRF ex, L-PRF CM, or DPSC CM. L-PRF CM had pro-survival and proliferative effects on unstimulated and cytokine-stimulated iMACs. L-PRF CM stimulated the release of IL-6 and PGE2, and increased MMP-13, TIMP-1 and IL-6 mRNA levels in cytokine-stimulated iMACs. DPSC CM increased the survival and proliferation of unstimulated iMACs. In cytokine-stimulated iMACs, DPSC CM increased TIMP-1 gene expression, whereas it inhibited nitrite release in 3D culture. We showed promising effects of DPSCs in an in vitro OA model, as they undergo chondrogenesis in vitro, stimulate the survival of chondrocytes and have immunomodulatory effects.

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Section: Discussionsupporting

confidence: 54%

Therapeutic Potential of Dental Pulp Stem Cells and Leukocyte- and Platelet-Rich Fibrin for Osteoarthritis

Monaco

Gervois

Beaumont

et al. 2020

Cells

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“…Lots of basic science studies on cell cultures and animal models as well as human clinical trials supported the application of PRP. [26][27][28] Numbers studies have focused on its effect on multiple stem cells proliferation, [29][30][31][32] EnMSCs could be conveniently segregated from menstrual blood and expanded to create almost unlimited supplies. There are several advantages of EnMSCs, including embryoniclike properties, noninvasive isolation procedures, and improved function.…”

Section: Discussionmentioning

confidence: 99%

Investigation of platelet‐rich plasma in increasing proliferation and migration of endometrial mesenchymal stem cells and improving pregnancy outcome of patients with thin endometrium

Wang

Liu

Mou

et al. 2018

J of Cellular Biochemistry

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Background Platelet‐rich plasma (PRP) contains abundant growth factors and is gradually used in the field of reproduction. A thin endometrium is recognized as a critical factor in embryo implantation failure. Endometrial mesenchymal stem cells (EnMSCs), which were isolated from human menstrual blood, are highly proliferative and show multiple differentiation capacity. The current study was to investigate the effect of PRP on the proliferation and migration of EnMSCs, and the effectiveness of PRP in the treatment of patients with thin endometrium. Materials and Methods EnMSCs were treated with PRP in vitro, followed by measuring cell proliferation, migration, and adhesion by using CCK8, scratch, and adhesion test, respectively. Twenty patients undergoing in vitro fertilization (IVF) with refractory thin endometrium history were given PRP by infusion into the uterine cavity after the treatment of hormone replacement therapy (HRT). Results All components of PRP significantly stimulated the growth, migration, and adhesion of EnMSCs when compared with the negative control. Cell proliferation and migration were induced by PRP in a dose‐dependent manner with maximum proliferation at a 2% PRP dose. The clinical data showed that successful endometrial expansion and pregnancy were discovered in 12 patients after PRP infusion, and the pregnancy rate increased to 60%. Conclusion Intrauterine PRP infusion represents a new way for female patients with thin endometrium with poor response. This study lays the foundations for the potential treatment of thin endometrium with PRP in vivo.

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“…There have been many speculations made in recent scientific reviews upon the beneficial use of platelet‐based applications in musculoskeletal injuries . These previous reviews of clinical trials using PRP in orthopaedic injuries seem to consistently indicate insufficient results while they recognize that platelet‐based applications may hold promise in future applications.…”

Section: Platelet‐mediated Skeletal Muscle Regenerationmentioning

confidence: 99%

“…Readers interested in the clinical aspects of platelet‐based applications for orthopaedic regeneration, in muscle, ligaments and tendons, are directed to recent relevant reviews . In the present review, we assimilate current experimental evidence on the role of platelets as biomaterials in tissue regeneration, particularly in skeletal muscle.…”

Section: Introductionmentioning

confidence: 99%

Platelet biology in regenerative medicine of skeletal muscle

2018

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Platelet-based applications such as platelet-rich plasma (PRP) and platelet releasate have gained unprecedented attention in regenerative medicine across a variety of tissues as of late. The rationale behind utilizing PRP originates in the delivery of key cytokines and growth factors from α-granules to the targeted area, which in turn act as cell cycle regulators and promote the healing process across a variety of tissues. The aim of the present review is to assimilate current experimental evidence on the role of platelets as biomaterials in tissue regeneration, particularly in skeletal muscle, by integrating findings from human, animal and cell studies. This review is composed of 3 parts: firstly, we review key aspects of platelet biology that precede the preparation and use of platelet-related applications for tissue regeneration. Secondly, we critically discuss relevant evidence on platelet-mediated regeneration in skeletal muscle focusing on findings from (i) clinical trials, (ii) experimental animal studies and (iii) cell culture studies; and thirdly, we discuss the application of platelets in the regeneration of several other tissues including tendon, bone, liver, vessels and nerve. Finally, we review key technical variations in platelet preparation that may account for the large discrepancy in outcomes from different studies. This review provides an up-to-date reference tool for biomedical and clinical scientists involved in platelet-mediated tissue regenerative applications.

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Platelet-Rich Plasma Derived Growth Factors Contribute to Stem Cell Differentiation in Musculoskeletal Regeneration

Cited by 118 publications

References 71 publications

Therapeutic Potential of Dental Pulp Stem Cells and Leukocyte- and Platelet-Rich Fibrin for Osteoarthritis

Therapeutic Potential of Dental Pulp Stem Cells and Leukocyte- and Platelet-Rich Fibrin for Osteoarthritis

Investigation of platelet‐rich plasma in increasing proliferation and migration of endometrial mesenchymal stem cells and improving pregnancy outcome of patients with thin endometrium

Platelet biology in regenerative medicine of skeletal muscle

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