Platelet-rich plasma for tissue regeneration can be stored at room temperature for at least five days

Moore, Gary; Maloney, James C.; Archer, Robert A.; Brown, Kerri L; Mayger, Katarzyna; Bromidge, E; Najafi, Mitra F

doi:10.1080/09674845.2016.1233792

Cited by 16 publications

(19 citation statements)

References 39 publications

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“…Autologous platelet concentrates are widely used as bioactive surgical additives to decrease inflammation and increase the speed of healing processes [ 4 ]. Several different techniques have been developed to produce platelet concentrates from blood [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

Biological and Mechanical Properties of Platelet-Rich Fibrin Membranes after Thermal Manipulation and Preparation in a Single-Syringe Closed System

Kardos

Hornyák

Simon

et al. 2018

IJMS

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Platelet-rich fibrin (PRF) membrane is a three-dimensional biodegradable biopolymer, which consists of platelet derived growth factors enhancing cell adhesion and proliferation. It is widely used in soft and hard tissue regeneration, however, there are unresolved problems with its clinical application. Its preparation needs open handling of the membranes, it degrades easily, and it has a low tensile strength which does not hold a suture blocking wider clinical applications of PRF. Our aim was to produce a sterile, suturable, reproducible PRF membrane suitable for surgical intervention. We compared the biological and mechanical properties of PRF membranes created by the classical glass-tube and those that were created in a single-syringe closed system (hypACT Inject), which allowed aseptic preparation. HypACT Inject device produces a PRF membrane with better handling characteristics without compromising biological properties. Freeze-thawing resulted in significantly higher tensile strength and higher cell adhesion at a lower degradation rate of the membranes. Mesenchymal stem cells seeded onto PRF membranes readily proliferated on the surface of fresh, but even better on freeze/thawed or freeze-dried membranes. These data show that PRF membranes can be made sterile, more uniform and significantly stronger which makes it possible to use them as suturable surgical membranes.

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Section: Introductionmentioning

confidence: 99%

Biological and Mechanical Properties of Platelet-Rich Fibrin Membranes after Thermal Manipulation and Preparation in a Single-Syringe Closed System

Kardos

Hornyák

Simon

et al. 2018

IJMS

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“…Therefore, Bausset et al recommend injecting PRP within 3 h after preparation to avoid the loss of efficacy [ 24 ]. There is an opposite viewpoint, that PRP for tissue regeneration can be stored for at least 5 days [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Quality Assessment of Platelet-Rich Fibrin-Like Matrix Prepared from Whole Blood Samples after Extended Storage

et al. 2017

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The platelet-rich fibrin–like matrix (PRFM) is usually prepared onsite and immediately used for regenerative therapy. Nonetheless, to meet the clinical necessity of preserving the PRFM without quality deterioration, we developed a method for preparation of PRFMs from short-term-stored whole blood (WB) samples. In this study, to evaluate the practical expiration date of storage, we extended the storage time of WB samples from 2 to 7 days and assessed the quality of the resulting PRFMs. WB samples collected with acid-citrate-dextrose were stored with gentle agitation at ambient temperature. To prepare PRFMs, the stored WB samples were mixed with CaCl2 in glass tubes and centrifuged. Fibrin fiber networks, CD41 and CD62P expression, and Platelet Derived Growth Factor-BB (PDGF-BB) levels were examined by scanning electron microscopy (SEM), flow cytometry, and an Enzyme-Linked ImmunoSorbent Assay (ELISA), respectively. Long-term storage had no significant effect on either blood cell counts or platelet functions tested. The resulting PRFMs were visually identical to freshly prepared ones. PDGF-BB levels did not markedly decrease in a time-dependent manner. However, fibrin fibers gradually became thinner after storage. Although the coagulation activity may diminish, we propose that PRFMs can be prepared—without evident loss of quality—from WB samples stored for up to 7 days by our previously developed method.

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“…While earlier studies [14][15][16][25][26][27] have examined growth factor concentration, data on the effect of various storage conditions on the concentration of various growth factors in PRP are limited. In addition, none of these studies compared the effect of storage and activation on growth factor concentrations in PRP.…”

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confidence: 99%

Effect of Storage Conditions and Activation on Growth Factor Concentration in Platelet‐Rich Plasma

Kim

Bae

Park

et al. 2019

Journal Orthopaedic Research

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This study aimed to evaluate growth factor concentration in platelet‐rich plasma (PRP) (leukocyte‐rich PRP) based on storage temperature, duration of storage, and method of activation. PRP samples were stored at 24℃ (room temperature group), 4℃ (refrigerator group), and −70℃ (deep‐freezer group). In each temperature, four aliquots were prepared based on the time of analysis (immediately, 1, 3, and 7 days after preparation). After storage, concentrations of platelet‐derived growth factor‐AA (PDGF‐AA), transforming growth factor‐β (TGF‐β), vascular endothelial growth factor (VEGF), insulin‐like growth factor‐1 (IGF‐1), and fibroblast growth factor‐basic (FGF‐B) were assessed with/without activation using Quantikine colorimetric sandwich immunoassay kits. PRP was activated with 10% Triton‐X for PDGF‐AA, VEGF, FGF‐B, IGF‐1 measurement and sonication for TGF‐β1 measurement. Without activation, PDGF‐AA concentration was highest on day 7 in the room temperature group. With activation, the concentration of PDGF‐AA was constant over the observation period at all temperatures. Without activation, the TGF‐β1 concentration remained negligible over the observation period at all temperatures. However, with activation, TGF‐β1 gradually increased to its highest concentration on day 7 at all temperatures. Over the observation period, VEGF and IGF‐1 concentrations were constant with and without activation at all temperatures. Without activation, FGF‐B concentration increased, with the highest concentration observed on day 7 in the deep‐freezer group. With activation, FGF‐B concentration decreased after day 1 in the room temperature group. Growth factor concentration in PRP differed significantly based on storage temperature, duration of storage, and method of activation. Appropriate storage conditions and activation are important to optimize its effects on desired clinical outcomes. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 38:777‐784, 2020

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Platelet-rich plasma for tissue regeneration can be stored at room temperature for at least five days

Abstract: Although platelet integrity and PDGF-BB fell over time, the intermittently agitated non-oxygen permeable container appeared to retain better platelet integrity and function, and PDGF-BB release, than other storage conditions, with potential for clinical use for 5-8 days.

Cited by 16 publications

References 39 publications

Biological and Mechanical Properties of Platelet-Rich Fibrin Membranes after Thermal Manipulation and Preparation in a Single-Syringe Closed System

Biological and Mechanical Properties of Platelet-Rich Fibrin Membranes after Thermal Manipulation and Preparation in a Single-Syringe Closed System

Quality Assessment of Platelet-Rich Fibrin-Like Matrix Prepared from Whole Blood Samples after Extended Storage

Effect of Storage Conditions and Activation on Growth Factor Concentration in Platelet‐Rich Plasma

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