Platelet-rich plasma in umbilical cord blood reduces neuropathic pain in spinal cord injury by altering the expression of ATP receptors

Behroozi, Zahra; Ramezani, Fatemeh; Janzadeh, Atousa; Rahimi, Behnaz; Nasirinezhad, Farinaz

doi:10.1016/j.physbeh.2020.113186

Cited by 21 publications

(37 citation statements)

References 120 publications

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“…Also, HUCB-PRP dramatically increased MAG expression and decreased GSK3β, caspase3 and CSF-tau levels compared to the injury group. Previous studies showed that PRP promoted or/and help axonal regeneration and sprouting of axons in the SCI models (14,(26)(27)(28)(29)(30)69). Similar to the results from Salarinia's study (29) we showed that PRP injection reduced apoptosis which resulted in an increased in the MAG level and reduction in the destruction of oligodendrocytes.…”

Section: Discussionsupporting

confidence: 88%

“…The other possibility of bene cial effect of PRP injection could be through mTOR signaling pathway. Our previous study indicated that HUCB-PRP increases mTOR signaling (69). MTOR signaling (as an upstream protein for apoptosis and GSK-3β) regulates central neuronal myelination and oligodendrocyte differentiation (85,86) and inhibits apoptosis (87) and GSK3β expression (88).…”

Section: Discussionmentioning

confidence: 99%

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Intraspinal Injection of Platelet-Rich Plasma Promotes Regeneration of the Spinal Cord and Improves Locomotor Function by Modulating the Apoptosis and the Wnt Signaling Pathways

Behroozi

Ramezani

Nasirinezhad

2021

Preprint

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Background: There are complex mechanisms for reducing intrinsic repair ability and neuronal regeneration following spinal cord injury (SCI). Platelet-rich plasma (PRP) is a rich source of growth factors and has been used to stimulate regeneration of peripheral nerves in degenerationtive diseases. However, only a few studies have investigated the effects of PRP on the SCI models. We examined whether PRP derived from human umbilical cord blood (HUCB-PRP) could recover motor function in animals with spinal cord injury. We also investigate the role of Wnt signaling pathway.Methods: Ault male Wistar rats were randomly divided into 6 groups (n=60) as control, sham, SCI, vehicle (SCI+platelet-poor plasma), PRP2day (SCI+injection 2 days after SCI) and PRP14day (SCI+injection 14 days after SCI). SCI was performed at the T12-T13 level. BBB tests were done weekly after injury for six weeks. caspase3 expression was determined using the Immunohistochemistry technique. The expression of GSK3β, Tau and MAG were determined using the Western blot technique. Data were analyzed by PRISM & SPSS software. Results: PRP injected animals showed a higher locomotor function recovery than those in the SCI group (p<0.0001). The level of caspase3, GSK3β and CSF- Tau reduced and MAG level in the spinal cord increased by injection of HUCB-PRP in animals with spinal cord injury. Conclusions: Injection of HUCB-PRP enhanced hind limb locomotor performance by modulation of caspase3, GSK3β, tau and MAG expression. Using HUCB-PRP could be a new therapeutic option for recovering the motor function and axonal regeneration after spinal cord injury.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Intraspinal Injection of Platelet-Rich Plasma Promotes Regeneration of the Spinal Cord and Improves Locomotor Function by Modulating the Apoptosis and the Wnt Signaling Pathways

Behroozi

Ramezani

Nasirinezhad

2021

Preprint

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“…The method of inducing SCI and injection of nanoparticles were similar to that of Behroozi et al [19]. Animals were anesthetized by injection of a mixture of ketamine and xylazine (100 mg/kg and 10 mg/kg ip).…”

Section: Sci Model and Nanoparticle Injectionmentioning

confidence: 99%

Injection of Cerium Oxide Nanoparticles To Treat Spinal Cord Injury In Rats

Behroozi

Rahimi

Hamblin

et al. 2021

Preprint

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Introduction: The present study, investigated the local injection of cerium oxide nanoparticles (CeONPs) into Spinal cord injury (SCI) lesions in rats, and the effect on motor performance and neuropathic pain, together with biochemical markers.Methods: 36 adult male Wistar rats were divided into 4 groups: control group (healthy animals); sham group (laminectomy); SCI group (laminectomy+SCI induction); treatment group (laminectomy + SCI induction + intrathecal injection of 10 µL of CeONPs (1000 µg/mL) immediately after injury). SCI was induced by application of an aneurysm clip at the T12-T13 vertebral region. Immediately after SCI, CeONPs were injected into the treatment group with a Hamilton syringe and micropipet. H&E staining and measurement of the size of the cavity were performed after 6 weeks, and the BBB motor performance test and pain threshold test were performed weekly. GCSF expression, P44/42 MAPK (ERK1/ERK2), P-P44/42 MAPK (ERK1/ERK2), total Tau, total MAG, β-actin were evaluated after 6 weeks.Results: The BBB score and pain threshold improved in animals receiving CeONPs compared with SCI animals. The size of the cavity decreased in the treatment group. GCSF protein expression levels were similar in animals receiving CeONPs compared with the SCI group, but the expression of ERK1/ERK2 and phospho-ERK was lower compared with the SCI group. The expression levels of Tau and MAG were significantly increased in treated animals compared to the SCI group.Conclusion: The use of CeONPs in SCI could improve motor functional recovery, reduce pain and increase nerve cell regeneration.

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“…Spinal cord injury (SCI) not only causes motor dysfunction but also causes neuropathic pain (NP) that is resistant to medical treatment and severely affects the quality of life of patients (Behroozi et al, 2021; Pfyffer et al, 2020; G. Zhang & Yang, 2017). The prevalence of NP after SCI is reported to be 53% to 80% (Brown et al, 2021; Burke et al, 2017; Gruener et al, 2020; Hunt et al, 2021; Kim et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Systematic analysis of critical genes and pathways identified a signature of neuropathic pain after spinal cord injury

Bai

Zhang

et al. 2022

Eur J of Neuroscience

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Spinal cord injury (SCI) damages sensory systems, producing chronic neuropathic pain that is resistant to medical treatment. The specific mechanisms underlying SCI-induced neuropathic pain (SCI-NP) remain unclear, and protein biomarkers have not yet been integrated into diagnostic screening. To better understand the host molecular pathways involved in SCI-NP, we used the bioinformatics method, the PubMed database and bioinformatics methods to identify target genes and their associated pathways. We reviewed 2504 articles

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Platelet-rich plasma in umbilical cord blood reduces neuropathic pain in spinal cord injury by altering the expression of ATP receptors

Cited by 21 publications

References 120 publications

Intraspinal Injection of Platelet-Rich Plasma Promotes Regeneration of the Spinal Cord and Improves Locomotor Function by Modulating the Apoptosis and the Wnt Signaling Pathways

Intraspinal Injection of Platelet-Rich Plasma Promotes Regeneration of the Spinal Cord and Improves Locomotor Function by Modulating the Apoptosis and the Wnt Signaling Pathways

Injection of Cerium Oxide Nanoparticles To Treat Spinal Cord Injury In Rats

Systematic analysis of critical genes and pathways identified a signature of neuropathic pain after spinal cord injury

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