Platelet-Rich Plasma Increases Matrix Metalloproteinases in Cultures of Human Synovial Fibroblasts

Browning, Shawn; Weiser, Amiee; Woolf, Naruewan; Golish, S. Raymond; SanGiovanni, Thomas P; Scuderi, Gaetano J.; Carballo, Carolina; Hanna, Lewis S.

doi:10.2106/jbjs.k.01501

Cited by 75 publications

(54 citation statements)

References 27 publications

(33 reference statements)

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“…The clinical benefits of PPP in wound healing have been previously reported by Withman et al [37]. Browning et al [12] have shown that in contrast with PPP, PRP can accelerate catabolic process in joint-microenvironments due to the presence of catabolic cytokines in PRP and the upregulation of MMPs in fibroblast-like synoviocytes exposed to PRP. In our study there was no characterization of PPP to…”

Section: Discussionmentioning

confidence: 77%

“…Cytokines and matrix metalloproteinases (MMPs) obtained in PRP can harm articular cartilage. PRP was shown to contain a significantly higher number of anabolic and catabolic cytokines relative to platelet-poor plasma (PPP) [12]. Browning et al [12] demonstrated that fibroblast-like synoviocytes treated with PRP produced an increase in matrix metalloproteinases (MMPs) secretion, which may accelerate cartilage catabolism.…”

Section: Introductionmentioning

confidence: 99%

“…PRP was shown to contain a significantly higher number of anabolic and catabolic cytokines relative to platelet-poor plasma (PPP) [12]. Browning et al [12] demonstrated that fibroblast-like synoviocytes treated with PRP produced an increase in matrix metalloproteinases (MMPs) secretion, which may accelerate cartilage catabolism. It was concluded from this study that PPP did not cause similar responses, and that synoviocytes produced lower concentrations of the above mentioned mediators.…”

Section: Introductionmentioning

confidence: 99%

“…It was concluded from this study that PPP did not cause similar responses, and that synoviocytes produced lower concentrations of the above mentioned mediators. Currently, only a few studies have attempted to evaluate the effect of PPP, which is a by-product of the platelet-rich plasma preparation process [12,13]. Because PPP is rich in plasma proteins and contains native levels of fibrinogen, it can be utilized as a sealant, with adhesive and hemostatic powers.…”

Section: Introductionmentioning

confidence: 99%

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Effect of PRP and PPP on proliferation and migration of human chondrocytes and synoviocytes in vitro

et al. 2013

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Cartilage tissue engineering can provide substantial relief to people suffering from degenerative cartilage disease, such as osteoarthritis. The autologous platelet-rich plasma (PRP) application appears to improve cartilage healing due to its ability to positively influence cellular mechanisms, mainly in cells from synovium and cartilage. Primary cultures of human synovial fluid stem cells (synoviocytes, SCs) and chondrocytes (CCs) were exposed to various concentrations of non-activated PRP and plateletpoor plasma (PPP) prepared by apheresis. Cell proliferation and migration were evaluated in real-time with the non-invasive xCELLigence System. It was found that PRP had a similar effect on the growth of cells as fetal bovine serum (FBS). Surprisingly, our proliferation assay results indicated that 50% PPP had the largest effect on both cell types, with a statistically significant increase in cell number (P<0.001) compared to the (0% FBS) in vitro control. The migratory ability of SCs was significantly enhanced with 10% PRP and 0.8% hyaluronic acid (HA). HA also augmented migration of CCs. In summary, these results demonstrate that directed cell proliferation and migration are inducible in human articular CCs and SCs, and that both platelet-derived fractions may exert a positive effect and modulate several cell responses that are potentially involved in tissue integration during cartilage repair.

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Section: Discussionmentioning

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of PRP and PPP on proliferation and migration of human chondrocytes and synoviocytes in vitro

et al. 2013

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“…Recent studies have demonstrated that PRP is superior when compared with HA for the treatment of patients with OA of the knee [47,106,107]. However, PRP contains a nondifferentiated cocktail of anti-inflammatory, pro-inflammatory, anabolic, and catabolic mediators [108]. While the replication of the body's natural balance of growth factors used synergistically may work best, recent studies have shown that isolating or blocking particular cytokines may be optimal for treating patients with particular conditions as observed in spinal cord injuries [109].…”

Section: Figurementioning

confidence: 99%

Clinical Aspects of Regenerative Medicine

Sampson

Bemden

Aufiero

2015

Translational Regenerative Medicine

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Randomized, Placebo‐Controlled Analysis of the Knee Synovial Environment Following Platelet‐Rich Plasma Treatment for Knee Osteoarthritis

Tucker

Goetz

Duncan³

et al. 2021

PM&R

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Background Platelet‐rich‐plasma (PRP) is used to treat knee osteoarthritis; however, mechanistic evidence of PRP effectiveness for pain relief is limited. Objective To assess molecular biomarkers and mesenchymal stem cells (MSCs) in synovial fluid during PRP treatment of the osteoarthritic knee joint. Design Single blinded, randomized, placebo controlled pilot study. Setting Veterans Affairs Medical Center. Participants Seventeen participants with mild to moderate knee osteoarthritis were randomized in a 2:1 placebo‐controlled ratio, receiving PRP or saline (placebo) intra‐articular injection into the knee joint. Methods Knee synovial fluid was analyzed before the respective injections and again 10 days following injection. Participants were followed up to 12 months completing visual analog scale (VAS) and Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) questionnaires at intervals over that period. Main Outcome Measures The effects of PRP on synovial protein and MSC gene expression levels were measured by multiplex enzyme‐linked immunosorbent assay and quantitative polymerase chain reaction. Results Novel biomarkers including levels of interleukin (IL)‐5, IL‐6, IL‐10, and tumor necrosis factor‐α were measured in synovial fluid 10 days after PRP treatment. Altered gene expression profiles in MSCs from patients treated with PRP were observed for matrix metalloproteinases and inflammatory markers (IL‐6, IL‐8, CCL2, TNF‐α). A2M protease was significantly increased following PRP treatment (P = .005). WOMAC scores declined for up to 3 months from baseline levels and remained low at 6 and 12 months in the PRP group. In contrast, WOMAC scores for patients receiving the saline injection were relatively unchanged for up to 12 months. Conclusions We report significant changes for the biomarker A2M (P = .005) as well as differences in expression of cellular markers and postulate that PRP modulates the local knee synovial environment by altering the inflammatory milieu, matrix degradation, and angiogenic growth factors. The PRP treatment group had less pain and stiffness and improved function scores.

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Platelet-Rich Plasma Increases Matrix Metalloproteinases in Cultures of Human Synovial Fibroblasts

Cited by 75 publications

References 27 publications

Effect of PRP and PPP on proliferation and migration of human chondrocytes and synoviocytes in vitro

Effect of PRP and PPP on proliferation and migration of human chondrocytes and synoviocytes in vitro

Clinical Aspects of Regenerative Medicine

Randomized, Placebo‐Controlled Analysis of the Knee Synovial Environment Following Platelet‐Rich Plasma Treatment for Knee Osteoarthritis

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