Platelet rich plasma may enhance peripheral nerve regeneration after cyanoacrylate reanastomosis: a controlled blind study on rats

Elgazzar, R.F.; Mutabagani, M.A.; Abdelaal, S.E.; Sadakah, A.A.

doi:10.1016/j.ijom.2008.05.010

Cited by 66 publications

(47 citation statements)

References 37 publications

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“…23 Earlier studies had demonstrated that PRP has numerous growth factors that effect nerve regeneration after nerve injury. 11,20,26,27 Another advantage of PRP is that it can be obtained easily and used promptly during surgery.…”

Section: Discussionmentioning

confidence: 99%

Effect of neural‐induced mesenchymal stem cells and platelet‐rich plasma on facial nerve regeneration in an acute nerve injury model

et al. 2010

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The use of PRP and/or nMSCs promotes facial nerve regeneration in an animal model of facial nerve axotomy. The use of nMSCs showed no benefit over the use of PRP in facial nerve regeneration, but the combined use of PRP and nMSCs showed a greater beneficial effect than use of either alone. This study provides evidence for the potential clinical application of PRP and nMSCs in peripheral nerve regeneration of an acute nerve injury. Laryngoscope, 2010.

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Section: Discussionmentioning

confidence: 99%

Effect of neural‐induced mesenchymal stem cells and platelet‐rich plasma on facial nerve regeneration in an acute nerve injury model

et al. 2010

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“…PRP was isolated using a standard laboratory centrifuge (Precision Durafuge 100; Thermo Fisher Scientific) according to a previously established method. 58 Briefly, after centrifugation at 400 g for 10 min with no brake applied, the supernatant was sterilely pipetted to a second sterile centrifuge tube, which was then centrifuged at 800 g for 10 min with a brake applied at the end of the cycle. The supernatant of platelet-poor plasma was then removed, and the remaining PRP was returned to ice.…”

Section: Prp Harvest and Processingmentioning

confidence: 99%

Uncultured Marrow Mononuclear Cells Delivered Within Fibrin Glue Hydrogels to Porous Scaffolds Enhance Bone Regeneration Within Critical-Sized Rat Cranial Defects

Kretlow

Spicer

Jansen

et al. 2010

Tissue Engineering Part A

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For bone tissue engineering, the benefits of incorporating mesenchymal stem cells (MSCs) into porous scaffolds are well established. There is, however, little consensus on the effects of or need for MSC handling ex vivo. Culture and expansion of MSCs adds length and cost, and likely increases risk associated with treatment. We evaluated the effect of using uncultured bone marrow mononuclear cells (bmMNCs) encapsulated within fibrin glue hydrogels and seeded into porous scaffolds to regenerate bone over 12 weeks in an 8-mm-diameter, criticalsized rat cranial defect. A full factorial experimental design was used to evaluate bone formation within model poly(L-lactic acid) and corraline hydroxyapatite scaffolds with or without platelet-rich plasma (PRP) and bmMNCs. Mechanical push-out testing, microcomputed tomographical analyses, and histology were performed. PRP showed no benefit for bone formation. Cell-laden poly(L-lactic acid) scaffolds without PRP required significantly greater force to displace from surrounding tissues than control (cell-free) scaffolds, but no differences were observed during push-out testing of coral scaffolds. For bone volume formation as analyzed by microcomputed tomography, significant positive overall effects were observed with bmMNC incorporation. These data suggest that bmMNCs may provide therapeutic advantages in bone tissue engineering applications without the need for culture, expansion, and purification.

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“…In experimental studies, PRP was used in peripheral nerve lesions, promoting remyelination in the facial (91) and sciatic nerve of rats (8) . The application of PRP increases the number of nerve fibers after peripheral nerve lesions, and can produce a neurotrophic effect, stimulating the proliferation of Schwann cells and myelination, important components during peripheral nerve repair (6,101) . Data in literature on the effect of PRP on peripheral nerve regeneration are scarce, which stimulates the search for more precise information about its performance.…”

Section: Collagen and Laminin As Carriersmentioning

confidence: 99%

Regeneração de nervos periféricos: terapia celular e fatores neurotróficos

Sebben¹,

Lichtenfels²,

Silva

2011

Rev. bras. ortop.

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Peripheral nerve trauma results in functional loss in the innervated organ, and recovery without surgical intervention is rare. Many surgical techniques can be used for nerve repair. Among these, the tubulization technique can be highlighted: this allows regenerative factors to be introduced into the chamber. Cell therapy and tissue engineering have arisen as an alternative for stimulating and aiding peripheral nerve regeneration. Therefore, the aim of this review was to provide a survey and analysis on the results from experimental and clinical studies that used cell therapy and tissue engineering as tools for optimizing the regeneration process. The articles used came from the LILACS, Medline and SciELO scientific databases. Articles on the use of stem cells, Schwann cells, growth factors, collagen, laminin and platelet-rich plasma for peripheral nerve repair were summarized over the course Rev Bras Ortop. 2011;46(6):643-49 of the review. Based on these studies, it could be concluded that the use of stem cells derived from different sources presents promising results relating to nerve regeneration, because these cells have a capacity for neuronal differentiation, thus demonstrating effective functional results. The use of tubes containing bioactive elements with controlled release also optimizes the nerve repair, thus promoting greater myelination and axonal growth of peripheral nerves. Another promising treatment is the use of platelet-rich plasma, which not only releases growth factors that are important in nerve repair, but also serves as a carrier for exogenous factors, thereby stimulating the proliferation of specific cells for peripheral nerve repair.

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Platelet rich plasma may enhance peripheral nerve regeneration after cyanoacrylate reanastomosis: a controlled blind study on rats

Cited by 66 publications

References 37 publications

Effect of neural‐induced mesenchymal stem cells and platelet‐rich plasma on facial nerve regeneration in an acute nerve injury model

Effect of neural‐induced mesenchymal stem cells and platelet‐rich plasma on facial nerve regeneration in an acute nerve injury model

Uncultured Marrow Mononuclear Cells Delivered Within Fibrin Glue Hydrogels to Porous Scaffolds Enhance Bone Regeneration Within Critical-Sized Rat Cranial Defects

Regeneração de nervos periféricos: terapia celular e fatores neurotróficos

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