Platelet-Rich Plasma Powder: A New Preparation Method for the Standardization of Growth Factor Concentrations

Kieb, Matthias; Sander, Frank E. A.; Prinz, Cornelia; Adam, Stefanie; Mau-Möller, Anett; Bader, Rainer; Peters, Kirsten; Tischer, Thomas

doi:10.1177/0363546516674475

Cited by 50 publications

(52 citation statements)

References 46 publications

(58 reference statements)

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“…In an attempt to deliver such factors to RP patients, Arslan et al used subtenon injections of autologous platelet rich plasma (aPRP). PRP contains a high concentration of multiple growth factors including basic fibroblast growth factor (bFGF), vascular endothelial growth factor (VEGF), transforming growth factor (TGF-B1) and brain-derived neurotrophic factor (BDNF) along with pro- and anti-inflammatory cytokines [ 149 , 150 ]. However, as the PRP was not purified, the exact concentration of each component received by the patients is not certain.…”

Section: Clinical Trialsmentioning

confidence: 99%

Mechanisms of Photoreceptor Death in Retinitis Pigmentosa

Newton

Megaw

2020

Genes

146

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Retinitis pigmentosa (RP) is the most common cause of inherited blindness and is characterised by the progressive loss of retinal photoreceptors. However, RP is a highly heterogeneous disease and, while much progress has been made in developing gene replacement and gene editing treatments for RP, it is also necessary to develop treatments that are applicable to all causative mutations. Further understanding of the mechanisms leading to photoreceptor death is essential for the development of these treatments. Recent work has therefore focused on the role of apoptotic and non-apoptotic cell death pathways in RP and the various mechanisms that trigger these pathways in degenerating photoreceptors. In particular, several recent studies have begun to elucidate the role of microglia and innate immune response in the progression of RP. Here, we discuss some of the recent progress in understanding mechanisms of rod and cone photoreceptor death in RP and summarise recent clinical trials targeting these pathways.

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Section: Clinical Trialsmentioning

confidence: 99%

Mechanisms of Photoreceptor Death in Retinitis Pigmentosa

Newton

Megaw

2020

Genes

146

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“…Pooling the PRP of different patients has emerged as a safe option for the standardization of these products. To validate this methodology, Kieb et al [19] quantified GFs, in particular VEGF, β-FGF, PDGF-AB, TGF-β1, IGF-1, IL-1α, IL-1β and IL-1RA in peripheral blood, fresh PRP prepared using a commercial system, fresh-frozen irradiated PC, and irradiated FD-PC. The different preparations had similar levels of GFs overall, although IGF-1 and IL-1α could not be detected in FD-PCs, since they are plasma proteins, not platelet proteins.…”

Section: Lyophilized Prp Preservationmentioning

confidence: 99%

Freeze-Drying of Platelet-Rich Plasma: The Quest for Standardization

Andı́a

Perez‐Valle

Amo

et al. 2020

IJMS

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The complex biology of platelets and their involvement in tissue repair and inflammation have inspired the development of platelet-rich plasma (PRP) therapies for a broad array of medical needs. However, clinical advances are hampered by the fact that PRP products, doses and treatment protocols are far from being standardized. Freeze-drying PRP (FD-PRP) preserves platelet function, cytokine concentration and functionality, and has been proposed as a consistent method for product standardization and fabrication of an off-the-shelf product with improved stability and readiness for future uses. Here, we present the current state of experimental and clinical FD-PRP research in the different medical areas in which PRP has potential to meet prevailing medical needs. A systematic search, according to PRISMA (Preferred Reported Items for Systematic Reviews and Meta-Analyses) guidelines, showed that research is mostly focused on wound healing, i.e., developing combination products for ulcer management. Injectable hydrogels are investigated for lumbar fusion and knee conditions. In dentistry, combination products permit slow kinetics of growth factor release and functionalized membranes for guided bone regeneration.

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“…Standardization of the preparation and use of PRP can aid regulation of the storage and transport of PRP . At present, there are standardized PRP preparation methods using growth‐factor concentrations and platelet count as quality‐control parameters . The “ideal” standardized PRP method should unify all the conditions that affect PRP quality, but the cost is too high and it is unnecessary.…”

Section: Discussionmentioning

confidence: 99%

Application of standardized platelet‐rich plasma in elderly patients with complex wounds

Tian

Cheng

Chen

et al. 2019

Wound Repair Regeneration

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In recent years, autologous platelet-rich plasma (PRP) derivatives have been used widely in the regeneration and repair of tissue, but a standard definition and preparation method for PRP are lacking. We developed a standardized method using platelet indices as quality-control indicators for PRP preparation. Twenty-one elderly patients (9 males, 12 females) with complex wounds were treated with standardized platelet-rich plasma (S-PRP). The platelet count in PRP after the second centrifugation was 1,069-1,436 × 10 9 /L. We adjusted the platelet concentration in PRP after a second centrifugation to 1,000 × 10 9 /L according to a formula using platelet-poor plasma (PPP). The standardized preparation method that we developed gave S-PRP with a relatively uniform platelet concentration. The wounds of 21 patients showed accelerated healing after S-PRP treatment, and there were no obvious side effects during treatment. These data suggest that our preparation method of S-PRP, using platelet indices as quality-control indicators with platelet count of 1,000 × 10 9 /L could be used for the treatment of complex wounds in the elderly. The preparation method of S-PRP proposed in the present study may be a simple and effective method of PRP quality control.

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Platelet-Rich Plasma Powder: A New Preparation Method for the Standardization of Growth Factor Concentrations

Cited by 50 publications

References 46 publications

Mechanisms of Photoreceptor Death in Retinitis Pigmentosa

Mechanisms of Photoreceptor Death in Retinitis Pigmentosa

Freeze-Drying of Platelet-Rich Plasma: The Quest for Standardization

Application of standardized platelet‐rich plasma in elderly patients with complex wounds

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