Platelet-rich plasma (prp) and tendon healing: animal model

Abstract

IntroductionThe tendon is a tissue which does not heal easily. Recently, several studies have demonstrated the positive effects of platelets on the healing process of tendons. A local injection of platelet-rich plasma (PRP), which releases in situ many growth factors, has the potentiality to enhance the tendon healing process. The aim of our experiment was to ascertain by an original mechanical measure whether the use of PRP was of interest for accelerating the healing process of rats' Achilles tendons after surgical induced lesion. Methods A 5 mm defect was surgically induced in 90 rats' Achilles tendon. Rats were divided into two groups of 45: (A) control (no treatment) and (B) PRP treatment. Rats of group B received a PRP injection in situ after the surgery. Afterwards, rats of both groups were placed in their cages without immobilisation. After 5, 15 and 30 days, 10 traumatised Achilles tendons of each group were dissected and removed. Immediately after sampling, tendons were submitted to a biomechanical tensile test up to rupture, using a 'Cryo-jaw'. After that, transcriptomic analyses were made on the tendon samples, to study the expression of type III collagen, matrix metalloproteases and tenomodulin. A hydroxyproline dosage was done to quantify the collagen in the tendon during its healing process. Tendons of the 15 remaining rats of each group were subjected to a histological study, respectively at day 5, 15 and 30 (fi ve rats for each time). Results We demonstrated that the force necessary to induce tendon rupture during biomechanical tensile test study was

Clinical Response After Treatment of Knee Osteoarthritis With a Standardized, Closed-System, Low-Cost Platelet-Rich Plasma Product: 1-Year Outcomes

“…The amount of growth factors in the PRP product is highly dependent on platelet-enrichment, but also on the postprocessing or activation method (ie, mechanical [through a freeze-thaw process] vs chemical [calcium chloride]) and is also a matter of debate. Several studies show that these methods may be equivalent, 2,27,50 highlighting that the freeze-thaw method is a simple and clinically compliant approach to assure the availability of plateletderived growth factors and bioactive molecules in the final product. 15 Furthermore, a recent study suggests that frozen-thawed PRP should be preferred for chronic pathologies such as KOA, where injections have to be carried out more regularly.…”

Study protocol for double-blind, randomised placebo-controlled trial evaluating semitendinosus function and morbidity following tendon harvesting for anterior cruciate ligament reconstruction augmented by platelet-rich plasma

“…Many growth factors found in PRP/ACP are also involved in the healing process. [33][34][35][36] For patients undergoing ACLR, it is currently unknown if PRP/ACP has therapeutic effects on regeneration of the semitendinosus and its distal tendon. If so, it may limit the functional and neuromuscular deficits reported over the short-term and long-term following ACLR, 28 which would improve the current standard of care To date, no randomised controlled trial has evaluated the efficacy of PRP/ACP in augmenting the recovery of the donor semitendinosus muscle following ALCR.…”

“…Indeed, both tendons and muscles heal through a dynamic process, with stages of inflammation, cellular proliferation and subsequent tissue remodelling. Many growth factors found in PRP/ACP are also involved in the healing process 33–36. For patients undergoing ACLR, it is currently unknown if PRP/ACP has therapeutic effects on regeneration of the semitendinosus and its distal tendon.…”

Applications cliniques du plasma riche en plaquettes (PRP) dans les lésions tendineuses : revue de la littérature

“…De nombreux modèles animaux ont démontré la capacité du PRP à stimuler et à accélérer le processus physiologique de régénération tissulaire, notamment lors de ruptures de tendons d'Achille chez les rats et de lésions au niveau du tendon rotulien [31][32][33]. Une qualité tissulaire optimale nécessiterait selon certains auteurs l'applications de charges mécaniques appropriées en plus de l'administration du PRP [34].…”