Platelet Sequestration in Man. I. Methods*

Aster, Richard H.; Jandl, James H.

doi:10.1172/jci104970

Cited by 807 publications

(191 citation statements)

References 29 publications

Supporting

Mentioning

179

Contrasting

Unclassified

Order By: Relevance

“…Platelet aggregation was measured turbidmetrically with a Shimazu recording spectrophotometer W-50. The buffer solution (Tris-ACD buffer solution) used for measuring aggregation of platelets consists of 5 vol of the mixture of 115 mM NaCl, 15 mM KCl, 25 mM Tris (pH 7.4) and 5 mM glucose and one vol of ACD solution [6]. The mixture of 5.0 ml of a sample protein solution and 0.1 ml of the platelet suspension (1.6-2.0 X 10' cells/mm3) was preincubated at 37°C for 20 min and then its optical density at 600 nm was recorded with time, stirring the reaction mixture with a stirrer (1000 rev/min).…”

Section: Methodsmentioning

confidence: 99%

Isolation of plasma co‐factor in adenosine diphosphate‐induced aggregation of bovine platelets

et al. 1975

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

Isolation of plasma co‐factor in adenosine diphosphate‐induced aggregation of bovine platelets

et al. 1975

View full text Add to dashboard Cite

“…Blood was collected on acid citrate dextrose (1/S by vol.) (Aster and Jandl, 1964). Platelets were prepared according to the method of Ardlie et al (1971) and used within 4 h. For aggregation assays, platelets (SX108 in 400 pl 01 Tyrode's albumin buffer) were incubated for 1 min at 37°C before addition of the proteinase.…”

Section: Methodsmentioning

confidence: 99%

Afaâcytin, an αβ‐fibrinogenase from Cerastes cerastes (Horned Viper) Venom, Activates Purified Factor X and Induces Serotonin Release from Human Blood Platelets

Laraba-Djebari

Martin‐Eauclaire

Mauco

et al. 1995

European Journal of Biochemistry

View full text Add to dashboard Cite

Afalcytin, a proteinase with caseinolytic, arginine-esterase and amidase activities, was purified from the venom of Cprustes cerustes (horned viper) in two steps by gel filtration through Sephadex '375, then HPLC on carboxymethyl-cellulose. AfaBcytin has an isoelectric point of 6.25. and consists of two subunits, m and /], which have the same apparent molecular mass (40000) and are indistinguishable in the absence of reduction orkand deglycosylation. Subunit /) is constituted of two disulfide-linked polypeptidic chains, , O and f . The respective apparent molecular inass of the chains are 43000 (u), 35500 (/)) and 10200 (p') as determined by SDS/PAGE under reducing conditions. Both chains a and / l are N-glycosylated. The two chains have the same N-terminal sequence (20 residues) which is similar to those of other proteinases from snake venom. Susceptibility of afalcytin to diisopropyl fluorophosphate and benzamidine indicates the presence of a serine and an aspartic (or glutamic) acid residues in the catalytic site.Ca'+ appears to be required for structural cohesion of the afaBcytin molecule. Afalcytin exhibits apfibrinogenase and rx-fibrinase properties. It replaces missing factors VIII and IX in deficient plasmas, and activates purified human factor X into factor Xa. It releases serotonin from platelets and directly aggregates human (but not rabbit) blood platelets. Despite its thrombin-like characteristics, however, afalcytin is not inhibited by plasmatic thrombin inhibitors. The procoagulant properties of afalcytin therefore have potential clinical applications.

show abstract

“…There was no clinical evidence of bleeding, thromboembolism, valve dysfunction, or perivalvular leak in any patient. Platelet survival times were determined by using S'chromium-labeled platelets according to the method of Aster and Jandl(1964). Samples were taken sequentially on each of 10 consecutive days beginning with the injection of fresh radioactive donor platelets.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Platelet Kinetics following Porcine and Mechanical Valve Replacement

Lee

Joye

Rose

et al. 1981

Clinical Cardiology

View full text Add to dashboard Cite

Summary:Because thromboembolic pathogenesis in mechanical prosthetic valves (MP) might involve selective MP platelet consumption with consequent reduced platelet survival, it has been suggested that lowered platelet half-life (TI 1 2 ) detection provides identification of postoperative thrombotic risk. Porcine heterografts (PH) possess less thromboembolic hazards than MP; thus, comparative evaluation of PH versus MP platelet Tl/2 is germane to the test's thrombotic predictive value, yet platelet kinetics in PH require elucidation. Accordingly, 5khromium platelet Tl/2 was determined in 16 patients with substituted valves (10 PH and 6 MP). Average platelet Tl/2 in PH (2.9f0.6 d) was similar (p>0.05) to MP (3.2f0.6 d), and both were considerably lower (pC0.05) than platelet TI^ in normal subjects (NL, 5.1 f0.5 d). Furthermore, platelet T , p was below the lowest NL platelet T i p (4.0 d) in seven PH and three MP patients. Thus, frequency and extent of decreased platelet survival in PH are common and equal to MP. These results indicate that platelet half-life does not afford thromboembolic predict-* This work was supported in part by Program Project grant HL-14780 from the National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland, and by California chapters of the American Heart Association, Dallas, Texas, USA.This work was presented in part at the 52nd Scientific Sessions of the American Heart Association, November 14, 1979, Anaheim, California, USA. ability in patients with mechanical valves and, instead, favors prosthetic-induced physical trauma causing abnormal platelet survival.

show abstract

Platelet Sequestration in Man. I. Methods*

Cited by 807 publications

References 29 publications

Isolation of plasma co‐factor in adenosine diphosphate‐induced aggregation of bovine platelets

Isolation of plasma co‐factor in adenosine diphosphate‐induced aggregation of bovine platelets

Afaâcytin, an αβ‐fibrinogenase from Cerastes cerastes (Horned Viper) Venom, Activates Purified Factor X and Induces Serotonin Release from Human Blood Platelets

Evaluation of Platelet Kinetics following Porcine and Mechanical Valve Replacement

Contact Info

Product

Resources

About