Platelet storage solution improves the in vitro function of preserved platelet concentrate

Abstract

ViaCyte appears to protect platelets during storage, reflected by a low level of induced lesions. Platelets stored in ViaCyte maintain energy levels at their resting state, which preserves their ability to aggregate and secrete granule contents, and ensures the availability of additional platelets for activation upon in vitro challenge.

Buffy‐coat platelet variables and metabolism during storage in additive solutions or plasma

“…11 Although there are many methods to evaluate PLT quality in vitro, there is no "gold standard" that is the ultimate descriptor of PLT quality. Consequently, studies comparing buffy-coat PLTs with plasma or PAS are limited to contrasts among plasma and one or two additive solutions (ASs), [12][13][14] between two ASs, 15 or simply just one PAS alone 16 and present comparisons with only selected evaluation methods. In this study, the most robust methods in the recent literature are used, and a comprehensive comparison of PCs stored in plasma and three PASs is provided.…”

Development of a single-antigen magnetic bead assay (SAMBA) for the sensitive detection of HPA-1a alloantibodies using tag-engineered recombinant soluble β3 integrin

“…These serum samples may contain low-avidity HPA-1a alloantibodies, which might dissociate from platelets in the MAIPA due to excessive washing. Another explanation is that the conformational change and shedding of platelet GPIIb-IIIa (Hoffmeister et al, 2003;Karnicki et al, 2003) during storage or isolation could have led to an inappropriate platelet antibody detection. Due to the scarcity of NAIT samples containing HPA-1b alloantibodies, only one serum sample containing HPA-1b alloantibodies could be tested using rs β 3 -Pro33-coupled beads.…”

“…In addition, these procedures are not only costly, time-consuming, labor-intensive and difficult to standardize, but also require highly qualified laboratories and special expertise for interpretation of the results. Furthermore, a shedding of platelet glycoproteins may occur in capture assays using preserved platelets, resulting in an inappropriate detection of platelet antibodies (Hoffmeister et al, 2003;Karnicki et al, 2003). Since non-contiguous residues are vital for HPA-1a alloantibody binding and the development of a cost-effective assay is desirable, we established a recombinant soluble (rs) β 3 integrin-1a-based single-antigen magnetic bead assay (SAMBA) that allows for the rapid, sensitive and specific detection of HPA-1a alloantibodies.…”

A novel assay for the detection of anti-human platelet antigen antibodies (HPA-1a) based on peptide aptamer technology

“…We also analyzed a series of samples from 68 patients suffering from thrombopenic diseases ( [3][4][5][6][7][8][9][10]. In some of them, the MAIPA assay showed the presence of anti-HPA-1b, anti-HPA-3a, anti-HPA-5a or anti-HPA-5b antibodies (Table 1, line 4-9).…”

“…However, during their conservation, platelet glycoproteins may undergo a shedding process so that the platelet preparation might not be suitable to detect some platelet antibodies. 8,9 Although antibodies against HPA-1a antigen may still be detected after several months at low temperature, attempts to keep platelet glycoprotein expression at normal levels during long-term storage remain problematic. 10 Finally, new batches of platelets need to be collected from donors at regular intervals; this may cause results to vary between different laboratories.…”