Platelet von Willebrand factor: sweet resistance

Lenting, Peter J.; Denis, Cécile V.

doi:10.1182/blood-2013-10-533174

Cited by 6 publications

(4 citation statements)

References 9 publications

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“…Another contributor to the differences in thrombus formation rates is the fact that thermal damage can activate platelets and the coagulation process (22). The results showing portal veins with higher expression of vWF, a key protein in platelet aggregation and subsequent thrombus growth, supports this biologic mechanism (23). The discrepancy in flow pattern between vessel types, coupled with the underlying differences in coagulation protein and gene expression serve as potential contributors to vessel thrombosis patterns seen in this in vivo study.…”

Section: Discussion (800)mentioning

confidence: 53%

Potential Mechanisms of Vascular Thrombosis after Microwave Ablation in an in Vivo Liver

Chiang

Nickel

Kimple

et al. 2017

Journal of Vascular and Interventional Radiology

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Purpose The purpose of this study was to evaluate potential biological and thermal mechanisms for the observed differences in thrombosis rates between hepatic vessels during microwave ablation procedures. Materials and Methods Microwave ablation antennas were placed in single liver lobes of two in-vivo porcine (n=3 in each animal; n =6 total) liver models in the proximity of a large (>5mm) portal vein and hepatic veins. Each ablation was performed with 100 W for 5 minutes. Conventional ultrasound imaging and intravascular temperature probes were used to evaluate vessel patency and temperature changes during the ablation procedure. Vascular endothelium was harvested 1 hour after ablation and used to characterize genes and proteins associated with thrombosis in portal and hepatic veins. Results Targeted portal veins and hepatic veins did not show a significant difference between average vessel size (8.7 ± 2.4 mm vs 9.7 ± 3.4 mm; p=0.44). Portal veins within the microwave ablation zone thrombosed at a significantly higher rate compared to hepatic veins (54.5% vs 0.0%, p=0.0046). There was a negligible change in intravascular temperature in both portal and hepatic veins during the ablation procedure (0.2 ± 0.4 vs 0.6 ± 0.9 °C, p=0.46). Portal veins demonstrated significantly higher gene expression compared to hepatic veins in terms of fold-differences in thrombomodulin (2.9 ± 2.0; p=0.0001), von Willebrand Factor (7.6 ± 1.5; p=0.0001), endothelial protein C receptor (3.50 ± 0.49; p=0.0011) and plasminogen activator inhibitor (SERPINE1: 1.46 ± 0.05; p=0.0014). Western blot analysis showed significantly higher expression of von Willebrand Factor (2.32 ± 0.92; p=0.031) in portal veins compared to hepatic veins. Conclusions Large portal veins thrombose more frequently than hepatic veins during microwave ablation procedures. Biological differences in thrombogenicity, rather than heat transfer, between portal veins and hepatic veins may contribute to their different rates of thrombosis.

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Section: Discussion (800)mentioning

confidence: 53%

Potential Mechanisms of Vascular Thrombosis after Microwave Ablation in an in Vivo Liver

Chiang

Nickel

Kimple

et al. 2017

Journal of Vascular and Interventional Radiology

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“…This may result in the formation of platelet-derived VWF strings that may even be more resistant to ADAMTS13 proteolysis. 10,32 Similar to endothelial cell-derived VWF strings, VWF strings from platelets could recruit new platelets and inflammatory cells resulting in platelet/leukocyte plugs that block the microvasculature and contribute to the no-reflow phenomenon. Interestingly, such platelet derived-VWF strings have been shown in vitro.…”

Section: Discussionmentioning

confidence: 99%

Platelet-derived VWF is not essential for normal thrombosis and hemostasis but fosters ischemic stroke injury in mice

Verhenne

Denorme

Libbrecht

et al. 2015

Blood

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“…When the ADAMT13 is lacking in vivo, as in Thrombotic Thrombocytopenic Purpura (TTP) disease, the clearance time and amount of VWF are abnormal, leading to microvascular thrombosis and organ ischemic infraction [ 50 ]. Prospective population-based cohort studies have revealed that low plasma ADAMT13 levels are related to risk and poor outcomes in AIS [ 51 , 52 ]. These studies suggested that both decreased amount of ADAMT13 and abnormal microenvironment would affect ULVWF elimination.…”

Section: Discussionmentioning

confidence: 99%

Platelet morphology, ultrastructure and function changes in acute ischemic stroke patients based on structured illumination microscopy

Yang¹,

Wang²,

Hu³

et al. 2023

Heliyon

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Platelet von Willebrand factor: sweet resistance

Cited by 6 publications

References 9 publications

Potential Mechanisms of Vascular Thrombosis after Microwave Ablation in an in Vivo Liver

Potential Mechanisms of Vascular Thrombosis after Microwave Ablation in an in Vivo Liver

Platelet-derived VWF is not essential for normal thrombosis and hemostasis but fosters ischemic stroke injury in mice

Platelet morphology, ultrastructure and function changes in acute ischemic stroke patients based on structured illumination microscopy

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