Platelet von Willebrand Factor — Collagen Binding Activity and Ristocetin Cofactor Activity: A Comparison of Methods Able to Differentiate von Willebrand Disease Types I and II (Preliminary Results)

Thomas, Kathy B.; Choong, S. C.; Jessat, U.; Zieger, Barbara; Budde, Ulrich; Tune, E. P.; Grohmann, A.; Sutor, A. H.

doi:10.1007/978-3-642-79009-6_38

Cited by 3 publications

(9 citation statements)

References 25 publications

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“…In comparison with the RCof determinations, the vWF:CBA PDC gave higher results and the vWF:CBA CF lower results, which is in good agreement with observations reported by other authors for plasma samples from patients with vWD. 11,13 VWF multimer analysis of the FVIII/vWF concentrates showed no correlation between the degree of vWF multimerization and vWF:CBA PDC results, which pivots to an increased binding of low vWF multimers in the vWF:CBA PDC compared with the vWF:CBA CF (Fig. 2).…”

Section: Discussionmentioning

confidence: 96%

“…vWF:CBA methods have been used by other groups for the analysis of plasma samples from patients with vWD 13 and FVIII/vWF concentrates. 15 The objective of the present study was to compare two vWF:CBAs that were distinctive with regard to the structure and type of collagen and the coating conditions used.…”

Section: Discussionmentioning

confidence: 99%

“…7466, 7509, 7547, and 7365 and a batch of a high-purity FVIII/vWF concentrate were measured with a commercial vWF:Ag ELISA (Asserachrom vWF, Boehringer Mannheim, Germany) according to the manufacturer's instructions. Additionally, we modified a previously published 13 vWF:Ag ELISA for research purposes, for example, the determination of vWF:Ag in heparin affinity chromatography fractions. Results from both vWF:Ag ELISAs were in good agreement (data not shown).…”

Section: Heparin Affinity Chromatographymentioning

confidence: 99%

“…The vWF:CBA CF was performed as described earlier 12,13 with the following modifications. Equine collagen fibrils type I (Helena Biosciences; 50 µg/mL Horm buffer, Nycomed) were adsorbed onto microtiter plates (MaxiSorp, Nunc, Germany).…”

Section: Vwf:cba Cfmentioning

confidence: 99%

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Comparison of Two von Willebrand Factor Collagen-Binding Assays with Different Binding Affinities for Low, Medium, and High Multimers of von Willebrand Factor

Neugebauer

Goy²,

Budek³

et al. 2002

Semin Thromb Hemost

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The use of von Willebrand factor collagen-binding assay (vWF:CBA) as an alternative method for the quantification of the physiological activity of vWF in plasma from patients with von Willebrand disease (vWD) and in blood clotting factor (F) VIII concentrates (FVIII/vWF concentrates) for the therapy of vWD is currently being discussed. We compared two vWF:CBAs that are distinctive with regard to the type and structure of collagen and the coating principle used. After analyzing samples of a plasma pool from normal donors, we received results that were in very good compliance with both methods. However, significantly different results (p < or =0.005) were obtained when FVIII/vWF concentrates were tested. In an attempt to elucidate these discrepancies, vWF multimers were separated by heparin affinity chromatography and analyzed by vWF antigen enzyme-linked immunosorbent assay (ELISA), ristocetin cofactor activity test, both vWF:CBAs, and a multimer analysis. From our data we conclude that the assay with pepsin-digested collagen (human, type III) that was covalently linked to preactivated microtiter plates (vWF:CBAPDC) revealed a higher affinity for low and medium vWF multimers, whereas the assay with collagen fibrils (equine, type I) that were adsorbed to microtiter plates (vWF:CBACF) predominantly bound high vWF multimers. Based on results reported by others, we assume that the discrepancies between both vWF:CBAs were not related to the type and species of collagen used. Taken together our results imply rather that fragmentation of collagen by pepsin digestion or subsequent covalent linkage to the microtiter plate, or both, increased the affinity for low and medium vWF multimers, whereas the fibrillar structure of collagen was required for the binding of high vWF multimers, which exhibit the highest physiological activity in primary hemostasis.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Heparin Affinity Chromatographymentioning

confidence: 99%

Section: Vwf:cba Cfmentioning

confidence: 99%

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Comparison of Two von Willebrand Factor Collagen-Binding Assays with Different Binding Affinities for Low, Medium, and High Multimers of von Willebrand Factor

Neugebauer

Goy²,

Budek³

et al. 2002

Semin Thromb Hemost

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show abstract

“…However, so far no EurPharm method has been fixed for a VWF:CBA. The expert group 6B considered two alternative CBAs and evaluated comparative data obtained by a collaborative study: One method, CBAECF, was a modification of a method described earlier [21,22], established in the PEI, using native equine tendon type I collagen fibrils. The other method, CBAHPDC, used a commercially available kit [23], with human placenta-derived pepsin-treated soluble type III collagen.…”

Section: Vwfmentioning

confidence: 99%

Standardisation of Plasma Products

Seitz¹

2003

Transfus Med Hemother

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In the past two decades, enormous efforts of all involved parties lead to a very significant improvement of quality, safety and efficacy of plasma derivatives. To achieve this, high standards were developed, implemented and controlled by the industry and regulatory authorities. A particular challenge is the standardisation of test methods such as the potency assays for Factor VIII and von Willebrand factor. Further work is needed in order to develop our test repertoire for adequate control of ‘old’ and novel products.

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Function of von Willebrand Factor in Children with Diarrhea-Associated Hemolytic-Uremic Syndrome (D+ HUS)

Sutor¹,

Thomas²,

Prüfer³

et al. 2001

Semin Thromb Hemost

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Reports on von Willebrand factor (vWF) in hemolytic-uremic syndrome (HUS) are not unequivocal. Because of potential pathogenic implications, we examined the ability of vWF to bind to collagen in vitro, which reflects its function. Plasma vWF antigen (vWF:Ag) and collagen-binding activity (vWF:CBA) were measured by enzyme-linked immunosorbent assay in children with (1) diarrhea-associated (D+) HUS (n = 27), (2) chronic renal insufficiency (CRI) (n = 8), (3) gastroenteritis (GE) not associated with HUS (n = 15), (4) immune thrombocytopenia (ITP) (n = 40) and from controls (n = 35). Structural vWF was evaluated by multimer analysis. Children with D+ HUS had vWF:Ag of 2.53 and vWF:CBA of 1.98 U/mL. The corresponding values for patients with ITP were 1.35 and 1.82 U/mL, with CRI 1.55 and 1.55 U/mL, and with GE 1.68 and 2.10 U/mL; all values were higher than in controls (1.04 and 1.16 U/mL). The mean ratio of vWF:CBA to vWF:Ag ratio in controls was 1.13; only children with HUS had a dysfunctional vWF, as indicated by a low ratio of 0.78; the ratio was elevated in children with ITP (1.36) and GE (1.27) and was normal in those with CRI (1.06). No ultralarge molecular multimers of vWF were detected in any group, including HUS. The very high concentration of plasma vWF:Ag in HUS probably reflects endothelial cell damage or irritation. In contrast to all other groups, only children with HUS had a dysfunctional vWF, caused either by a primary (due to enterohemorrhagic Escherichia coli) or secondary (due to consumption of functionally active vWF) process. This abnormality was not obvious as structural anomaly by multimer analysis.

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Platelet von Willebrand Factor — Collagen Binding Activity and Ristocetin Cofactor Activity: A Comparison of Methods Able to Differentiate von Willebrand Disease Types I and II (Preliminary Results)

Cited by 3 publications

References 25 publications

Comparison of Two von Willebrand Factor Collagen-Binding Assays with Different Binding Affinities for Low, Medium, and High Multimers of von Willebrand Factor

Comparison of Two von Willebrand Factor Collagen-Binding Assays with Different Binding Affinities for Low, Medium, and High Multimers of von Willebrand Factor

Standardisation of Plasma Products

Function of von Willebrand Factor in Children with Diarrhea-Associated Hemolytic-Uremic Syndrome (D+ HUS)

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