Platelet X-Ray Microanalysis in Patients with Chronic Renal Failure

Djaldetti, M; Fishman, Pnina; Creter, D; Zahavi, Izhar; Zevin, D; Levi, J

doi:10.1159/000206221

Cited by 5 publications

(9 citation statements)

References 16 publications

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“…However, the present paper and three previous reports [27][28][29] from other laboratories have shown normal collagen-induced platelet aggregation in the plasma of patients undergoing mainte nance hemodialysis because of chronic renal failure.…”

Section: Discussioncontrasting

confidence: 59%

Cigarette-Smoking Affects Platelets and Endothelium in Chronic Hemodialyis Patients

Davis

Arnold

Wiegmann

1993

Nephron

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Previous work has shown that cigarette-smoking affects platelets and endothelium in men and women without renal failure. The present study compared 10 male habitual smokers receiving maintenance hemodialysis for chronic renal failure with 11 healthy male habitual smokers before and after controlled cigarette-smoking after a 12-hour period of abstinence. Collagen-induced platelet aggregation was not significantly different in the two groups of men before smoking. The platelet aggregate ratio was lower and the plasma concentration of platelet factor 4 was higher in the dialysis patients than in the healthy men (p < 0.05). Mean or median values before and after cigarette smoking by the dialysis patients were 42 and 62 chart units for collagen-induced platelet aggregation, 0.88 and 0.77 for the platelet aggregate ratio, 6.2 and 8.5 ng/ml for the plasma concentration of platelet factor 4, 0.8 and 1.7 per counting chamber for the endothelial cell count and 4 and 37 ng/ml for the plasma nicotine concentration (each p < 0.01). None of the changes which occurred in the dialysis patients was statistically significantly different from those in the healthy men. We conclude that cigarette-smoking has similar effects on platelets and endothelium in patients receiving maintenance hemodialysis and in healthy controls.

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Section: Discussioncontrasting

confidence: 59%

Cigarette-Smoking Affects Platelets and Endothelium in Chronic Hemodialyis Patients

Davis

Arnold

Wiegmann

1993

Nephron

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“…Various techniques, such as atomic absorption spectrometry (AAS) [ 10-141, neutron activation (NA) [ 15-171, and X-ray microprobe analysis [18][19][20][21], have been utilized to determine the level of Zn and other elements in whole platelets. The most sensitive techniques (NA and AAS) indicate that whole platelets contain a substantial pool of Zn.…”

Section: Introductionmentioning

confidence: 99%

Platelet multielemental composition, lability, and subcellular localization

Gorodetsky¹,

Mou²,

Blankenfeld

et al. 1993

American J Hematol

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Diagnostic X-ray spectrometry (DXS), based on X-ray fluorescence, was used to quantitate directly the multiple elemental composition of washed, intact human platelets (n = 16), with the following results: K = 3.08 +/- 1.00 mg/g, Ca = 1.18 +/- 0.29 mg/g, Zn = 35 +/- 9 micrograms/g. These values show that washed platelets contain significant pools of K, Ca, and Zn, the latter some 30-60-fold higher than plasma levels. Dialysis of whole platelets against cation exchange resin (Chelex-100) did not extract Ca(II) and Zn(II) sequestered within whole cells. To identify the subcellular locale of the elements, platelet lysate was subjected to 30-70% sucrose gradient ultracentrifugation and subcellular enriched fractions were obtained. Fractions were analyzed by DXS (for elements), electron microscopy (for dense granules), and subcellular markers fibrinogen and von Willebrand factor. In contrast to Ca and K, which accumulate in the dense granules and the cytoplasm, respectively, Zn appears to be distributed in the alpha-granules (40%) and the cytoplasm (60%). The subcellular distribution of Zn(II) is discussed within the context of the sensitivity of platelet response to the availability of Zn(II) and the platelet release reactions following stimulation.

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“…Since the results of platelet calcium concentration in the present work were expressed as percentages, we have chosen to examine only two elements, namely Ca and P, although a large number of other elements have been detected by this method [11], We believe that when the element is present in a relatively low concentration, the determination of a lower number of elements will produce better results.…”

Section: Discussionmentioning

confidence: 99%

Effect of Verapamil on Platelet Surface Ultrastructure and Calcium Content

Djaldetti

Gilgal²,

Fishman³

et al. 1988

Acta Haematol

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The effect of verapamil on the surface ultrastructure and calcium content of human platelets was studied by scanning electron microscopy and X-ray microanalysis. The results showed that the drug prevents platelet pseudopodia formation and causes a dose-dependent inhibition of platelet calcium concentration. The significance of these findings in the explanation of the inhibitory effect of verapamil on platelet aggregation is discussed.

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Platelet X-Ray Microanalysis in Patients with Chronic Renal Failure

Cited by 5 publications

References 16 publications

Cigarette-Smoking Affects Platelets and Endothelium in Chronic Hemodialyis Patients

Cigarette-Smoking Affects Platelets and Endothelium in Chronic Hemodialyis Patients

Platelet multielemental composition, lability, and subcellular localization

Effect of Verapamil on Platelet Surface Ultrastructure and Calcium Content

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