Platelets: a potential role in chronic respiratory diseases?

Chebbo, Mohamad; Duez, Catherine; Alessi, Marie‐Christine; Chanez, Pascal; Gras, Delphine

doi:10.1183/16000617.0062-2021

Cited by 17 publications

(14 citation statements)

References 158 publications

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“…Severe asthma, one of the most common inflammatory lung diseases, is characterised by uncontrolled recruitment and activation of inflammatory cells within the airways. 2,3 The inflammation of bronchial epithelium cells and their interaction with immune cells, particularly PLT, are well documented in asthma [5][6][7]10,11,13,[15][16][17] ; and PLT activation has been demonstrated by PLT mediators release in BAL and peripheral blood from patients with severe asthma. [8][9][10][11] We investigated an airway inflammation marker expressed both by bronchial epithelium cells and by peripheral circulating PLT in an inflammatory context such as asthma, to get a better insight of inflammation onset and possible epithelial-immune cells positive loop in chronic lung disease.…”

Section: Discussionmentioning

confidence: 99%

“…Human bronchial epithelium cells (HBEC) play a main role in immune cells response. Among these, platelets (PLT), anucleate cells implicated in haemostasis and in inflammation, 4–6 appear to have a preferential interaction with lung tissue. In asthma context, HBEC and PLT interaction is supported by PLT activation markers (including PF4 and β‐thromboglobulin) present in broncho‐alveolar lavage (BAL) or the peripheral blood of asthmatic patients 7–10 .…”

Section: Introductionmentioning

confidence: 99%

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Mobilisation of HLA‐F on the surface of bronchial epithelial cells and platelets in asthmatic patients

Fiouane

Chebbo

Beley

et al. 2022

HLA

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Uncontrolled inflammation of the airways in chronic obstructive lung diseases leads to exacerbation, accelerated lung dysfunction and respiratory insufficiency. Among these diseases, asthma affects 358 million people worldwide. Human bronchial epithelium cells (HBEC) express both antiinflammatory and activating molecules, and their deregulated expression contribute to immune cell recruitment and activation, especially platelets (PLT) particularly involved in lung tissue inflammation in asthma context.Previous results supported that HLA-G dysregulation in lung tissue is associated with immune cell activation. We investigated here HLA-F expression, reported to be mobilised on immune cell surface upon activation and displaying its highest affinity for the KIR3DS1-activating NK receptor. We explored HLA-F transcriptional expression in HBEC; HLA-F total expression in PBMC and HBEC collected from healthy individuals at rest and upon chemical activation and HLA-F membrane expression in PBMC, HBEC and PLT collected from healthy individuals at rest and upon chemical activation. We compared HLA-F transcriptional expression in HBEC from healthy individuals and asthmatic patients and its surface expression in HBEC and PLT from healthy individuals and asthmatic patients. Our results support that HLA-F is expressed by HBEC and PLT under healthy physiological conditions and is retained in cytoplasm, barely expressed on the surface, as previously reported in immune cells. In both cell types, HLA-F reaches the surface in the inflammatory asthma context whereas no effect is observed at the transcriptional level. Our study suggests that HLA-F surface expression is a ubiquitous post-transcriptional process in activated cells. It may be of therapeutic interest in controlling lung inflammation.Sabrina Fiouane and Mohamad Chebbo contributed equally to this work.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mobilisation of HLA‐F on the surface of bronchial epithelial cells and platelets in asthmatic patients

Fiouane

Chebbo

Beley

et al. 2022

HLA

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“…However, platelets have always been a difficult blood cell to work with in the research setting. They are not easy to manipulate because of their relatively small size, their lack of nucleus and their highly sensitive nature [ 1 ]. Platelet preparations obtained by classical purification method are generally contaminated by numbers of leukocytes and erythrocytes [ 9 ].…”

Section: Discussionmentioning

confidence: 99%

“…For many years, platelets were considered as megakaryocyte-derived fragments exclusively involved in hemostasis and thrombosis. However, recent studies revealed that platelets may play a key role in several biological processes especially immune response, using their proteins and modulators pre-synthetized in megakaryocytes or internalized from blood by endocytosis [ 1 ]. Interestingly, these cells also contain a reservoir of RNAs derived from megakaryocytes and all the machinery useful for mRNA translation, allowing platelets to translate different mRNA onto proteins in response to various stimulants [ 2 , 3 ].…”

Section: Introductionmentioning

confidence: 99%

Platelets Purification Is a Crucial Step for Transcriptomic Analysis

Chebbo

Assou

Pantesco

et al. 2022

IJMS

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Platelets are small anucleate cells derived from the fragmentation of megakaryocytes and are involved in different biological processes especially hemostasis, thrombosis, and immune response. Despite their lack of nucleus, platelets contain a reservoir of megakaryocyte-derived RNAs and all the machinery useful for mRNA translation. Interestingly, platelet transcriptome was analyzed in health and diseases and led to the identification of disease-specific molecular signatures. Platelet contamination by leukocytes and erythrocytes during platelet purification is a major problem in transcriptomic analysis and the presence of few contaminants in platelet preparation could strongly alter transcriptome results. Since contaminant impacts on platelet transcriptome remains theoretical, we aimed to determine whether low leukocyte and erythrocyte contamination could cause great or only minor changes in platelet transcriptome. Using microarray technique, we compared the transcriptome of platelets from the same donor, purified by common centrifugation method or using magnetic microbeads to eliminate contaminating cells. We found that platelet transcriptome was greatly altered by contaminants, as the relative amount of 8274 transcripts was different between compared samples. We observed an increase of transcripts related to leukocytes and erythrocytes in platelet purified without microbeads, while platelet specific transcripts were falsely reduced. In conclusion, serious precautions should be taken during platelet purification process for transcriptomic analysis, in order to avoid platelets contamination and result alteration.

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“…Platelets also effectively contribute to chronic respiratory diseases like asthma [ 152 , 153 ], chronic obstructive pulmonary disease (COPD) [ 154 ] and aspirin-exacerbated respiratory disease (AERD) [ 155 ]. Platelet-derived inflammatory mediators, platelet interaction with inflammatory cells of the innate (monocyte, neutrophils) or the adaptive immune wing play a decisive role in disease progression and severity [ 156 ]. Some of these pathologies involve platelet activation through FcγRIIa as an effector mechanism in instigating a thrombo-inflammatory response [ 85 , 86 ], while platelet derived TxA 2 may induce bronchoconstriction [ 157 ] and cysteinyl leukotrienes produced during transcellular lipid metabolism involving platelets and leukocytes [ 158 ] can exaggerate the inflammatory processes.…”

Section: Therapeutic Implication Of Ackr3/cxcr7 In Platelet-associated Cardiovascular Diseasementioning

confidence: 99%

Atypical Roles of the Chemokine Receptor ACKR3/CXCR7 in Platelet Pathophysiology

Chatterjee

2022

Cells

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The manifold actions of the pro-inflammatory and regenerative chemokine CXCL12/SDF-1α are executed through the canonical GProteinCoupledReceptor CXCR4, and the non-canonical ACKR3/CXCR7. Platelets express CXCR4, ACKR3/CXCR7, and are a vital source of CXCL12/SDF-1α themselves. In recent years, a regulatory impact of the CXCL12-CXCR4-CXCR7 axis on platelet biogenesis, i.e., megakaryopoiesis, thrombotic and thrombo-inflammatory actions have been revealed through experimental and clinical studies. Platelet surface expression of ACKR3/CXCR7 is significantly enhanced following myocardial infarction (MI) in acute coronary syndrome (ACS) patients, and is also associated with improved functional recovery and prognosis. The therapeutic implications of ACKR3/CXCR7 in myocardial regeneration and improved recovery following an ischemic episode, are well documented. Cardiomyocytes, cardiac-fibroblasts, endothelial lining of the blood vessels perfusing the heart, besides infiltrating platelets and monocytes, all express ACKR3/CXCR7. This review recapitulates ligand induced differential trafficking of platelet CXCR4-ACKR3/CXCR7 affecting their surface availability, and in regulating thrombo-inflammatory platelet functions and survival through CXCR4 or ACKR3/CXCR7. It emphasizes the pro-thrombotic influence of CXCL12/SDF-1α exerted through CXCR4, as opposed to the anti-thrombotic impact of ACKR3/CXCR7. Offering an innovative translational perspective, this review also discusses the advantages and challenges of utilizing ACKR3/CXCR7 as a potential anti-thrombotic strategy in platelet-associated cardiovascular disorders, particularly in coronary artery disease (CAD) patients post-MI.

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Platelets: a potential role in chronic respiratory diseases?

Cited by 17 publications

References 158 publications

Mobilisation of HLA‐F on the surface of bronchial epithelial cells and platelets in asthmatic patients

Mobilisation of HLA‐F on the surface of bronchial epithelial cells and platelets in asthmatic patients

Platelets Purification Is a Crucial Step for Transcriptomic Analysis

Atypical Roles of the Chemokine Receptor ACKR3/CXCR7 in Platelet Pathophysiology

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