Platelets mitochondrial function depends on CoQ10 concentration in winter, not in spring season

Abstract

Seasonal variations in temperature may influence the physiological and pathological metabolic pathways, concentrations of antioxidants, degree of oxidative stress and mitochondrial function. The aim of this study was to evaluate platelet mitochondrial function in human subjects during seasonal variations in temperature. Two groups of healthy young subjects were enroled in the study. Winter group, mean outside temperature was 4.77°C and Spring group, mean outside temperature was 24.32°C. High-resolution respirometry method was used for determination of mitochondrial respiration and oxidative phosphorylation in platelets. Concentrations of coenzyme Q 10 (CoQ 10) and tocopherols were determined in platelets, blood and plasma. Our data showed slightly (not significantly) reduced respiration in intact platelets, basal and ADP-stimulated mitochondrial respiration at Complex I, as well as CoQ 10-TOTAL and α-tocopherol concentrations in winter. The concentration of γ-tocopherol was higher in winter. Platelet mitochondrial ATP production depended on platelet CoQ 10-TOTAL concentration in winter, not in spring. We conclude that seasonal temperature participates in the mechanism of platelet mitochondrial respiratory chain function and oxidative phosphorylation that depends on their CoQ 10-TOTAL concentration at lower winter outside temperature. CoQ 10 supplementation may improve platelets mitochondrial ATP production at winter season. High-resolution respirometry offers sensitive method for detection of changes of platelets mitochondrial respiratory function.

Platelet mitochondrial respiration and coenzyme Q10 could be used as new diagnostic strategy for mitochondrial dysfunction in rheumatoid diseases

“…Platelet mitochondrial dysfunction has been documented in patients with several human diseases [ 18 ] including Alzheimer´s disease [ 22 ], depression [ 23 ], chronic kidney disease [ 24 – 26 ], patients after kidney transplantation [ 27 ]. Platelet mitochondrial dysfunction has also been observed in human ageing [ 28 ] and in winter time [ 29 ]. Data on platelet mitochondrial function in patients with RA are not available.…”