Platform- and label-free detection of lead ions in environmental and laboratory samples using G-quadraplex probes by circular dichroism spectroscopy

Kim, Raeyeong; Youn, Young-Sang; Kang, Misook; Kim, Eun-Joo

doi:10.1038/s41598-020-77449-5

Cited by 8 publications

(15 citation statements)

References 41 publications

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“…A more pressing concern is the ability to distinguish Pb 2+ from K + because natural water samples typically contain appreciable quantities of potassium, which can potentially suppress a probe response for the presence of lead and generate a false-negative readout . Recently, Kim and co-workers utilized CD to determine a K d of 830 nM for Pb 2+ -binding by native TBA in the presence of 100 μM K + ion, which increased to 220 μM in the presence of 100 mM K + . Native TBA binds K + ion with a K d of 5000 nM (from mass spectrometry)13 000 nM (from CD titration) depending on the conditions.…”

Section: Resultsmentioning

confidence: 99%

“…A lead ion also more strongly stabilizes H-bonds through polarization enhancement (Figure B), with a diagnostic long-wavelength circular dichroism (CD) maximum at ∼310 nm rather than ∼290 nm for monocationic antiparallel GQs . This feature has been employed by Kim and co-workers to monitor Pb 2+ -binding using the 15mer thrombin binding aptamer (TBA, 5′-G 2 T 2 G 3 TGTG 2 T 2 G 2 -3′) as a CD-specific label-free sensor …”

Section: Introductionmentioning

confidence: 99%

“…10 This feature has been employed by Kim using the 15mer thrombin binding aptamer (TBA, 5′-G 2 T 2 G 3 TGTG 2 T 2 G 2 -3′) as a CD-specific label-free sensor. 23 Recently, we incorporated a molecular rotor cyanine− indole−quinolinium (4QI) hemicyanine dye tethered to an acyclic 1,2-propanediol linker into the T3-loop position of TBA to monitor probe response to thrombin binding by the antiparallel GQ motif. 24 Although the 4QI surrogate provided a turn-off response to thrombin binding, it displayed impressive fluorescence brightness (ε.Φ fl ∼ 5400 cm −1 M −1 ) within TBA compared to free dye binding to the antiparallel GQ (∼11-fold increase in intensity for an internal label versus free dye) and had a minimal impact on the stability and structure of the antiparallel GQ bound by K + .…”

Section: ■ Introductionmentioning

confidence: 99%

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On-Strand Knoevenagel Insertion of a Hemicyanine Molecular Rotor Loop Residue for Turn-On Fluorescence Detection of Pb-Induced G-Quadruplex Rigidity

2021

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We demonstrate the ability to distinguish Pb 2+ from K + within the central cavity of the antiparallel G-quadruplex (GQ) DNA produced by the thrombin binding aptamer (TBA) using an internal molecular rotor fluorescent probe. An indole−aldehyde containing an acyclic N-glycol group was first employed in the on-strand Knoevenagel condensation with five different heterocyclic quaternary cationic acceptors to assess the molecular rotor character of the resulting cyanine−styryl dyes within duplex DNA. An indole−pyridinium (4PI) nucleobase surrogate displayed the greatest turn-on emission response to duplex formation and was thus inserted into the loop residues of TBA to monitor GQ-folding in the presence of Pb 2+ versus K + . TBA-4PI exhibits turn-on emission upon Pb 2+ -binding with a brightness (ε•Φ fl ) of 9000 cm −1 M −1 compared to K +binding (ε•Φ fl ∼ 2000 cm −1 M −1 ) due to Pb 2+ -induced GQ rigidity with 4PI-Gtetrad stacking interactions. The Pb 2+ -bound TBA-4PI GQ also provides energytransfer (ET) fluorescence with a diagnostic excitation at 310 nm for distinguishing Pb 2+ from K + within the antiparallel GQ. The TBA-4PI GQ affords the desired turn-on fluorescence response for detecting Pb 2+ ions with an apparent dissociation constant (K d ) of 63 nM and a limit of detection (LOD) of 19 nM in an aqueous buffer. It can also distinguish Pb 2+ (230 nM) from K + (1.5 mM, 6500-fold excess) in an antiparallel GQ recognition motif without topology twitching.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

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On-Strand Knoevenagel Insertion of a Hemicyanine Molecular Rotor Loop Residue for Turn-On Fluorescence Detection of Pb-Induced G-Quadruplex Rigidity

2021

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“…Other sources include lead battery manufacturers, lead-based pigment manufacturers, automobile industries, decayed water-carrying metal pipes and contaminated consumer products [ 20 ]. Exposure to lead at a certain concentration has been implicated with several health problems such as renal dysfunction, nervous system impairment, headache, fatigue, constipation and brain development impairment in children [ 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Novel Aminosilane (APTES)-Grafted Polyaniline@Graphene Oxide (PANI-GO) Nanocomposite for Electrochemical Sensor

et al. 2021

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Lead is a potentially toxic element (PTE) that has several adverse medical effects in humans. Its presence in the environment became prominent due to anthropogenic activities. The current study explores the use of newly developed composite materials (organic–inorganic hybrid) based on PANI-GO-APTES for electrochemical detection of Pb2+ in aqueous solution. The composite material (PANI-GO-APTES) was synthesized by chemical method and was characterized with SEM, XPS, XEDS, XRD, TGA, FTIR, EIS and CV. The result of characterization indicates the successful synthesis of the intended material. The PANI-GO-APTES was successfully applied for electrochemical detection of Pb2+ using cyclic voltammetry and linear sweep voltammetry method. The limit of detection of Pb2+ was 0.0053 µM in the linear range of 0.01 µM to 0.4 µM. The current response produced during the electrochemical reduction of Pb2+ catalyzed by PANI-GO-APTES was also very repeatable, reproducible and rapid. The application of PANI-GO-APTES-modified GCE in real sample analysis was also established. Therefore, PANI-GO-APTES is presented as a potential Pb2+ sensor for environmental and human health safety.

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“…Circular dichroism (CD) is very sensitive to secondary structure of nucleic acid [ 64 ]. The spectral shape and signal of CD can effectively show the secondary structure changes of DNA caused by the binding to small molecules [ 65 ]. Isothermal titration calorimetry (ITC) is an intuitive and unlabeled method for analyzing and characterizing molecules which can directly measure the thermal changes caused by the chemical reactions between the two components [ 66 , 67 ].…”

Section: Selection Of Heavy Metal Ion Aptamersmentioning

confidence: 99%

Advances in aptamer screening and aptasensors’ detection of heavy metal ions

et al. 2021

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Heavy metal pollution has become more and more serious with industrial development and resource exploitation. Because heavy metal ions are difficult to be biodegraded, they accumulate in the human body and cause serious threat to human health. However, the conventional methods to detect heavy metal ions are more strictly to the requirements by detection equipment, sample pretreatment, experimental environment, etc. Aptasensor has the advantages of strong specificity, high sensitivity and simple preparation to detect small molecules, which provides a new direction platform in the detection of heavy metal ions. This paper reviews the selection of aptamers as target for heavy metal ions since the 21th century and aptasensors application for detection of heavy metal ions that were reported in the past five years. Firstly, the selection methods for aptamers with high specificity and high affinity are introduced. Construction methods and research progress on sensor based aptamers as recognition element are also introduced systematically. Finally, the challenges and future opportunities of aptasensors in detecting heavy metal ions are discussed.

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Platform- and label-free detection of lead ions in environmental and laboratory samples using G-quadraplex probes by circular dichroism spectroscopy

Cited by 8 publications

References 41 publications

On-Strand Knoevenagel Insertion of a Hemicyanine Molecular Rotor Loop Residue for Turn-On Fluorescence Detection of Pb-Induced G-Quadruplex Rigidity

On-Strand Knoevenagel Insertion of a Hemicyanine Molecular Rotor Loop Residue for Turn-On Fluorescence Detection of Pb-Induced G-Quadruplex Rigidity

Novel Aminosilane (APTES)-Grafted Polyaniline@Graphene Oxide (PANI-GO) Nanocomposite for Electrochemical Sensor

Advances in aptamer screening and aptasensors’ detection of heavy metal ions

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