Platform for quantitative multiscale imaging of tissue composition

Pinkert, Michael A.; Simmons, Z. J.; Niemeier, Ryan C.; Dai, Bing; Woods, Lauren B.; Hall, Timothy J.; Campagnola, Paul J.; Rogers, Jeremy D.; Eliceiri, Kevin W.

doi:10.1364/boe.383248

Cited by 4 publications

(4 citation statements)

References 66 publications

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“…By using single platform to achieve multi-level imaging, image registration could be eased. A recent study described a multiscale imaging platform that is sensitive to differences in tissue structure ranging from the organ macroscale to the subcellular nanoscale [ 121 ]. Five modalities were incorporated onto the integrated platform: ultrasound, second harmonic generation microscopy, multiphoton microscopy, optical coherence tomography, and enhanced backscattering.…”

Section: Multiscale Integration and Fusion Platform Of “Transpathology”mentioning

confidence: 99%

“… A multiscale imaging platform was developed and this system simplified the task of comparing like structures between modalities. [ 121 ] MRI, μMRI, μCT Angiogenesis in a mouse breast cancer model The resolution gap between ex vivo μCT and in vivo MRI was bridged using intermediate resolution ex vivo μMRI. An integrated platform was developed for characterizing angiogenesis at multiple spatial scales in a human breast cancer model.…”

Section: Multiscale Integration and Fusion Platform Of “Transpathology”mentioning

confidence: 99%

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Transpathology: molecular imaging-based pathology

Tian

Jin

et al. 2021

Eur J Nucl Med Mol Imaging

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Pathology is the medical specialty concerned with the study of the disease nature and causes, playing a key role in bridging basic researches and clinical medicine. In the course of development, pathology has significantly expanded our understanding of disease, and exerted enormous impact on the management of patients. However, challenges facing pathology, the inherent invasiveness of pathological practice and the persistent concerns on the sample representativeness, constitute its limitations. Molecular imaging is a noninvasive technique to visualize, characterize, and measure biological processes at the molecular level in living subjects. With the continuous development of equipment and probes, molecular imaging has enabled an increasingly precise evaluation of pathophysiological changes. A new pathophysiology visualization system based on molecular imaging is forming and shows the great potential to reform the pathological practice. Several improvements in “trans-,” including trans-scale, transparency, and translation, would be driven by this new kind of pathological practice. Pathological changes could be evaluated in a trans-scale imaging mode; tissues could be transparentized to better present the underlying pathophysiological information; and the translational processes of basic research to the clinical practice would be better facilitated. Thus, transpathology would greatly facilitate in deciphering the pathophysiological events in a multiscale perspective, and supporting the precision medicine in the future.

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Section: Multiscale Integration and Fusion Platform Of “Transpathology”mentioning

confidence: 99%

Section: Multiscale Integration and Fusion Platform Of “Transpathology”mentioning

confidence: 99%

Transpathology: molecular imaging-based pathology

Tian

Jin

et al. 2021

Eur J Nucl Med Mol Imaging

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“…This section describes the US and the SHG modules of the system. The full description of the system with other optical imaging modalities is detailed in a prior article (Pinkert et al 2020b).…”

Section: System Descriptionmentioning

confidence: 99%

“…We have recently developed a multiscale-imaging platform for registered qualitative ultrasound and SHG (Pinkert et al 2020b), but there are three additional challenges to performing QUS it did not address. The first challenge is determining system-specific settings necessary for QUS, as the multiscale-imaging platform uses a Verasonics research system with high-frequency transducers (18.5 and 28.5 MHz) that, to our knowledge, have not been used for QUS in the literature.…”

Section: Introductionmentioning

confidence: 99%

Challenges of conducting quantitative ultrasound with a multimodal optical imaging system

Pinkert¹,

Hall²,

Eliceiri³

2021

Phys. Med. Biol.

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High-frequency quantitative ultrasound is a potential non-invasive source of imaging cell-tissue scale biomarkers for major diseases such as heart disease, cancer, and preterm birth. However, one of the barriers to developing such biomarkers is that it is labor-intensive to compare quantitative ultrasound images to optical images of the tissue structure. We have previously developed a multiscale imaging system that can obtain registered qualitative ultrasound and optical images, but there are further technical challenges to obtaining quantitative data: System-specific details of obtaining and processing data with Verasonics high-frequency transducers; the need for high-frequency reference phantoms; and off-axis clutter from imaging above a glass coverslip. This paper provides a characterization of the Verasonics ultrasound system with the 18.5 MHz L22-14v and 28.5 MHz L38-22v transducers, describes the construction of high-frequency reference phantoms, and details methods for reducing off-axis clutter. The paper features a demonstration multiscale image of a wild type mouse mammary gland that incorporates quantitative ultrasound with both transducers and second harmonic generation microscopy. These advances demonstrate a way to obtain, on a single system with a cohesive and integrated pipeline, quantitative ultrasound data that is correlated with optical imaging without the need for extensive sample preparation.

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