Platycodon grandiflorum polysaccharide induces dendritic cell maturation via TLR4 signaling

Park, Ju Hee; Ryu, Hwa Sun; Kim, Ji Sung; Lee, Hong Kyung; Kang, Jong Soon; Yun, Jieun; Kim, Sung Yeon; Lee, Mi Kyeong; Hong, Jin Tae; Kim, Youngsoo; Han, Sang‐Bae

doi:10.1016/j.fct.2014.07.011

Cited by 62 publications

(28 citation statements)

References 47 publications

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“…The research on PG mainly focused on its biological activities, including its anti-tumor, anti-oxidation, and immune-enhancing effects. PG polysaccharides have immunostimulatory activity ( Zheng et al, 2017 ), induce DC maturation and increase the expression levels of MHC-I/II, co-stimulatory molecules and cytokines, which can activate T cells ( Park et al, 2014 ; Zheng et al, 2017 ). However, the effects of PG polysaccharides on the distribution of GA have remained elusive.…”

Section: Introductionmentioning

confidence: 99%

An Optimized MicroPET Imaging Method for the Distribution and Synergies of Natural Products

et al. 2018

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Purpose: Understanding the distribution and interaction of the Traditional Chinese Medicines (TCMs) is an integral source of herbal drug discovery. An optimized radio-labeled method was explored that could conduct in situ biodistribution studies in animals. We evaluated the feasibility of the method and applied glycyrrhetinic acid and platycodon (PG) polysaccharides as models.Procedures: [18F]-GA is a novel radiotracer which was performed positron emission tomography (PET) studies to assay the biodistribution of GA in mice. In addition, PG polysaccharide was used to intervene the biodistribution and dosimetry of GA. Scanning data were analyzed with professional software.Results: Record the time-activity curves for all organs then use the normalization method to calculate the area under the curve as a dosimetry for each organ. Moreover, the addition of PG polysaccharides can significantly improve the dosimetry of GA in the lungs, and its effect was related to the administration time.Conclusion: MicroPET imaging opens up a new avenue for the application of drug interactions between the TCMs.

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Section: Introductionmentioning

confidence: 99%

An Optimized MicroPET Imaging Method for the Distribution and Synergies of Natural Products

et al. 2018

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“…Next, to investigate whether DP6 stimulates the activation of MAPKs, AKT, and NF-κB, which are crucial for TLR4-mediated DC activation ( 10 ), the phosphorylation levels of MAPKs and AKT and the degradation levels of p65 in response to DP6 were identified in DCs from WT and TLR4 −/− mice ( Fig. 2C ).…”

Section: Resultsmentioning

confidence: 99%

Neoagarohexaose-mediated activation of dendritic cells via Toll-like receptor 4 leads to stimulation of natural killer cells and enhancement of antitumor immunity

Lee¹,

Jang²,

Yoon³

et al. 2017

BMB Reports

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β-Agarase cleaves the β-1,4 linkages of agar to produce neoagarooligosaccharides (NAO), which are associated with various physiological functions. However, the immunological functions of NAO are still unclear. In this study, we demonstrated that β-agarase DagA-produced neoagarohexaose (DP6), an NAO product, promoted the maturation of dendritic cells (DCs) by Toll-like receptor 4 (TLR4). DP6 directly and indirectly enhanced the activation of natural killer (NK) cells in a TLR4-dependent manner in vitro and in vivo. Finally, the antitumor activity of DP6 against B16F1 melanoma cells was inhibited in NK cell-depletion systems by using NK-cell depleting antibodies in vivo. Collectively, the results indicated that DP6 augments antitumor immunity against B16F1 melanoma cells via the activation of DC-mediated NK cells in a TLR4-dependent manner. Thus, DP6 is a potential candidate adjuvant that acts as an immune cell modulator for the treatment of melanoma.

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“…DCs as a kind of professional antigen presentation cells (APC) that can capture and process antigens, and proteases to initiate an immune response [ 42 , 43 ]. The effect of DCs to modulate immunity is dependent on DC maturation.…”

Section: Resultsmentioning

confidence: 99%

Phosphorylated Radix Cyathulae officinalis Polysaccharides Act as Adjuvant via Promoting Dendritic Cell Maturation

et al. 2017

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The aim of this study was to investigate whether phosphorylated Radix Cyathulae officinalis Kuan polysaccharides (pRCPS) used as adjuvant with foot-and-mouth disease vaccine (FMDV) can stimulate specific humoral and cellular immune responses in ICR mice. The results demonstrated that pRCPS significantly up-regulated FMDV-specific IgG, IgG1, IgG2b and IgG2a antibody levels and splenocyte proliferation. pRCPS also promoted the killing activities of cytotoxic T lymphocytes (CTL) and natural killer cells (NK). In addition, pRCPS enhanced the expression levels of IL-2, IL-4, and IFN-γ in CD4+ T cells and the level of IFN-γ in CD8+ T cells. Importantly, pRCPS enhanced the expression of MHCII, CD40+, CD86+, and CD80+ in dendritic cells (DCs). This study indicated that phosphorylation modification could increase immune-enhancing activities of RCPS, and pRCPS could promote humoral and cellular immune responses through facilitating DC maturation.

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Platycodon grandiflorum polysaccharide induces dendritic cell maturation via TLR4 signaling

Cited by 62 publications

References 47 publications

An Optimized MicroPET Imaging Method for the Distribution and Synergies of Natural Products

An Optimized MicroPET Imaging Method for the Distribution and Synergies of Natural Products

Neoagarohexaose-mediated activation of dendritic cells via Toll-like receptor 4 leads to stimulation of natural killer cells and enhancement of antitumor immunity

Phosphorylated Radix Cyathulae officinalis Polysaccharides Act as Adjuvant via Promoting Dendritic Cell Maturation

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