PLC/PRF/5 (Alexander) hepatoma cell line: further characterization and studies of infectivity

Daemer, Richard J.; Feinstone, S. M.; Alexander, J. J.; Tully, Joseph G.; London, William T.; Wong, Doris C.; Purcell, Robert H.

doi:10.1128/iai.30.2.607-611.1980

Cited by 24 publications

(2 citation statements)

References 21 publications

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“…In this research, PLC/PRF/5 human hepatocellular carcinoma cell line was used to investigate the anticancer effects of the standard compounds and extracts. The cell line is a well-characterized hepatocellular carcinoma containing hotspot p53 mutation at R249, which is a unique hotspot codon of liver cancer [ 6 , 36 ]. Though previous studies have reported the effect of hesperidin or limonin on HepG2 cells, liver cancer cell lines with wild-type p53 gene [ 16 , 17 ], this is the first study to report the effect of hesperidin, limonin, combination, and the ethanolic extract of the lime peel on liver cancer cells with hotspot mutated p53.…”

Section: Discussionmentioning

confidence: 99%

Metabolomic Analysis of Phytochemical Compounds from Ethanolic Extract of Lime (Citrus aurantifolia) Peel and Its Anti-Cancer Effects against Human Hepatocellular Carcinoma Cells

2023

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Lime peels are food waste from lime product manufacturing. We previously developed and optimized a green extraction method for hesperidin-limonin-rich lime peel extract. This study aimed to identify the metabolomics profile of phytochemicals and the anti-cancer effects of ethanolic extract of lime (Citrus aurantifolia) peel against liver cancer cells PLC/PRF/5. The extract’s metabolomics profile was analyzed by using LC-qTOF/MS and GC-HRMS. The anti-cancer effects were studied by using MTT assay, Annexin-PI assay, and Transwell-invasion assay. Results show that the average IC50(s) of hesperidin, limonin, and the extract on cancer cells’ viability were 165.615, 188.073, and 503.004 µg/mL, respectively. At the IC50 levels, the extract induced more apoptosis than those of pure compounds when incubating for 24 and 48 h (p < 0.0001). A combination of limonin and hesperidin showed a synergistic effect on apoptosis induction (p < 0.001), but the effect of the combination was still less than that of the extract at 48 h. Furthermore, the extract significantly inhibited cancer cell invasion better than limonin but equal to hesperidin. At the IC50 level, the extract contains many folds lower amounts of hesperidin and limonin than the IC50 doses of the pure compounds. Besides limonin and hesperidin, there were another 60 and 22 compounds detected from the LCMS and GCMS analyses, respectively. Taken altogether, the superior effect of the ethanolic extract against liver cancer cells compared to pure compound likely results from the combinatorial effects of limonin, hesperidin, and other phytochemical components in the extract.

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Section: Discussionmentioning

confidence: 99%

Metabolomic Analysis of Phytochemical Compounds from Ethanolic Extract of Lime (Citrus aurantifolia) Peel and Its Anti-Cancer Effects against Human Hepatocellular Carcinoma Cells

2023

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“…It was sourced from a 24 years Shangaan male with HCC from Mozambique (Figure 3A). This cell line continuously produces the HBsAg, however there was no expression for other markers of HBV synthesis [38]. Some oncogenes like c-Myc, c-abl, c-ha-ras c-fes, c-fms, and c-sis oncogenes are indicated in the cell line along with HBsAg.…”

Section: Plc/prf/5(alexander)mentioning

confidence: 96%

Origin and properties of hepatocellular carcinoma cell lines

Puttahanumantharayappa¹,

Gowda²,

Shiragannavar³

et al. 2021

Japanese J Gastroenterol Res

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The liver is one of the vital organs in human beings. It is responsible for several functions such as energy homeostasis, detoxification, regulating blood sugar levels, protein synthesis, bile acid secretion, cholesterol production and glycogen storage. Hepatocellular Carcinoma (HCC) alternately referred to as malignant hepatoma accounts to ~75% of all liver cancers and its resistance to therapeutics at progressed stages makes it lethal. Some of the main causes of HCC include HBV, HCV, aflatoxin, chronic alcoholism, excess nutrition, liver cirrhosis, iron over load, Wilson's disease, and type 2 diabetes. It is essential to detect the underlying causes of HCC at the cellular and molecular levels to develop drugs and find a potential cure for the disease. Several ongoing studies on HCC cell lines help to understand the gene expressions, multiple signaling pathways and differential drug responses to HCC. This review article provides insights to the possible molecular pathways involved in the origin of commonly used HCC cell lines and the signals that are functionally active in them. Keywords: hepatocellular carcinoma; malignant; cell lines; molecular pathways; Msignals.

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IgM Antibody to Hepatitis B Core Antigen in Korean Patients with Hepatocellular Carcinoma† ‡

et al. 1984

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Primary hepatocellular carcinoma (PHC) has been linked etiologically to persistent hepatitis B virus (HBV) infections by epidemiologic, serologic and molecular lines of evidence. To evaluate the frequency of IgM antibody to the viral core antigen (IgM anti-HBc) detected by a highly sensitive radioimmunoassay, we compared 110 Korean patients with PHC to a group of 63 age- and sex-matched control patients with other tumors. Results were correlated with those of commercially available HBV assays. IgM anti-HBc was found in 74 of 110 PHC patients (67%), but only 1 of 63 (1.6%) control patients. Although HBsAg was found in a larger percentage of PHC patients (81%), it was also present in more control patients (14%). Thus, IgM anti-HBc was more specifically associated with PHC than was the presence of HBsAg. IgM anti-HBc was found in 91% of PHC patients with detectable HBeAg and 74% of PHC patients with positive anti-HBe tests (p less than 0.04). The frequency of IgM anti-HBc was similar among HBsAg-positive PHC patients with and without anti-HBs, or those with low or high levels of serum alpha-fetoprotein. In 18 patients with PHC, IgM anti-HBc was further characterized by rate-zonal centrifugation of sera, all were found to have 19S IgM anti-HBc although 6 also had greater or equal IgM anti-HBc reactivity in the low molecular weight region. The presence of IgM anti-HBc in adult Korean HBsAg carriers may indicate an especially high risk for the development of PHC, and this should be evaluated in prospective studies.

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PLC/PRF/5 (Alexander) hepatoma cell line: further characterization and studies of infectivity

Cited by 24 publications

References 21 publications

Metabolomic Analysis of Phytochemical Compounds from Ethanolic Extract of Lime (Citrus aurantifolia) Peel and Its Anti-Cancer Effects against Human Hepatocellular Carcinoma Cells

Metabolomic Analysis of Phytochemical Compounds from Ethanolic Extract of Lime (Citrus aurantifolia) Peel and Its Anti-Cancer Effects against Human Hepatocellular Carcinoma Cells

Origin and properties of hepatocellular carcinoma cell lines

IgM Antibody to Hepatitis B Core Antigen in Korean Patients with Hepatocellular Carcinoma† ‡

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