Pleiotrophin increases neurite length and number of spiral ganglion neurons in vitro

Bertram, Sebastian; Roll, Lars; Reinhard, Jacqueline; Groß, Katharina; Dazert, Stefan; Faissner, Andréas; Volkenstein, Stefan

doi:10.1007/s00221-019-05644-6

Cited by 12 publications

(8 citation statements)

References 52 publications

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“…This observation is consistent with the geroscience paradigm that accelerated aging has a causative role in multiple chronic diseases (22). The protein most strongly associated with chronological age in the current study was pleiotrophin (PTN), which is a heparin-binding growth factor involved in angiogenesis, tumorigenesis, and neuromodulation (23,24). In our study, PTN was not predictive of either mortality or multimorbidity after adjusting for age.…”

Section: Mendelian Randomizationsupporting

confidence: 90%

Plasma proteomic biomarker signature of age predicts health and life span

Tanaka

Basisty

Fantoni

et al. 2020

eLife

113

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Older age is a strong shared risk factor for many chronic diseases and there is increasing interest in identifying aging biomarkers. Here a proteomic analysis of 1301 plasma proteins was conducted in 997 individuals between 21 and 102 years of age. We identified 651 proteins associated with age (506 over-represented, 145 underrepresented with age) was identified. Mediation analysis suggested a role for partial cis-epigenetic control of protein expression with age. Of the age-associated proteins, 33.5% and 45.3%, were associated with mortality and multimorbidity, respectively. There was enrichment of proteins associated with inflammation and extracellular matrix as well as senescence-associated secretory proteins. A 76-protein proteomic age signature predicted accumulation of chronic diseases and all-cause mortality. These data support the premise of proteomic biomarkers to monitor aging trajectories and to identify individuals at higher risk for disease to be targeted for in depth diagnostic procedures and early interventions.

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Section: Mendelian Randomizationsupporting

confidence: 90%

Plasma proteomic biomarker signature of age predicts health and life span

Tanaka

Basisty

Fantoni

et al. 2020

eLife

113

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“…In order to cover the entire SH-SY5Y cells that were seeded onto the coverslips, five visual fields including superior, central, inferior parts, as well as left and right-hand sides were randomly selected to count the number of immunopositive cells or measure the neurite length using Image J software. According to Bertram et al (2019), the neurite length was defined as the distance between the center of the cell soma and the tip of its longest neurite. The number of TH or BrdU or TH/BrdU-doubleimmunopositive cells, as well as the neurite length in each group, was expressed as an average value in five visual fields.…”

Section: Data Acquisition and Statistical Analysismentioning

confidence: 99%

RETRACTED: Allopregnanolone Modulates GABAAR-Dependent CaMKIIδ3 and BDNF to Protect SH-SY5Y Cells Against 6-OHDA-Induced Damage

Wang

Bian

et al. 2020

Front. Cell. Neurosci.

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Allopregnanolone (APα), as a functional neurosteroid, exhibits the neuroprotective effect on neurodegenerative diseases such as Parkinson's disease (PD) through γ-aminobutyric acid A receptor (GABAAR), but it has not been completely understood about its molecular mechanisms. In order to investigate the neuroprotective effect of APα, as well as to clarify its possible molecular mechanisms, SH-SY5Y neuronal cell lines were incubated with 6-hydroxydopamine (6-OHDA), which has been widely used as an in vitro model for PD, along with APα alone or in combination with GABAAR antagonist (bicuculline, Bic), intracellular Ca 2+ chelator (EGTA) and voltage-gated L-type Ca 2+ channel blocker (Nifedipine). The viability, proliferation, and differentiation of SH-SY5Y cells, the expression levels of calmodulin (CaM), Ca 2+ /calmodulin-dependent protein kinase II δ3 (CaMKIIδ3), cyclin-dependent kinase-1 (CDK1) and brain-derived neurotrophic factor (BDNF), as well as the interaction between CaMKIIδ3 and CDK1 or BDNF, were detected by morphological and molecular biological methodology. Our results found that the cell viability and the number of tyrosine hydroxylase (TH), bromodeoxyuridine (BrdU) and TH/BrdU-positive cells in 6-OHDA-treated SH-SY5Y cells were significantly decreased with the concomitant reduction in the expression levels of aforementioned proteins, which were ameliorated following APα administration. In addition, Bic could further increase the number of TH or BrdU-positive cells as well as the expression levels of aforementioned proteins except for TH/BrdU-double positive cells, while EGTA and Nifedipine could attenuate the expression levels of CaM, CaMKIIδ3 and BDNF. Moreover, there existed a direct interaction between CaMKIIδ3 and CDK1 or

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“…Glial cells migrate over long distances to form stable interconnections with neurons [ 156 , 157 , 158 , 159 , 160 , 161 ]. The netrin and semaphorin families have important guidance roles which control glial cell migration [ 162 , 163 , 164 ].…”

Section: The Semaphorinsmentioning

confidence: 99%

The CNS/PNS Extracellular Matrix Provides Instructive Guidance Cues to Neural Cells and Neuroregulatory Proteins in Neural Development and Repair

Melrose

Hayes

Bix

2021

IJMS

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Background. The extracellular matrix of the PNS/CNS is unusual in that it is dominated by glycosaminoglycans, especially hyaluronan, whose space filling and hydrating properties make essential contributions to the functional properties of this tissue. Hyaluronan has a relatively simple structure but its space-filling properties ensure micro-compartments are maintained in the brain ultrastructure, ensuring ionic niches and gradients are maintained for optimal cellular function. Hyaluronan has cell-instructive, anti-inflammatory properties and forms macro-molecular aggregates with the lectican CS-proteoglycans, forming dense protective perineuronal net structures that provide neural and synaptic plasticity and support cognitive learning. Aims. To highlight the central nervous system/peripheral nervous system (CNS/PNS) and its diverse extracellular and cell-associated proteoglycans that have cell-instructive properties regulating neural repair processes and functional recovery through interactions with cell adhesive molecules, receptors and neuroregulatory proteins. Despite a general lack of stabilising fibrillar collagenous and elastic structures in the CNS/PNS, a sophisticated dynamic extracellular matrix is nevertheless important in tissue form and function. Conclusions. This review provides examples of the sophistication of the CNS/PNS extracellular matrix, showing how it maintains homeostasis and regulates neural repair and regeneration.

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Pleiotrophin increases neurite length and number of spiral ganglion neurons in vitro

Cited by 12 publications

References 52 publications

Plasma proteomic biomarker signature of age predicts health and life span

Plasma proteomic biomarker signature of age predicts health and life span

RETRACTED: Allopregnanolone Modulates GABAAR-Dependent CaMKIIδ3 and BDNF to Protect SH-SY5Y Cells Against 6-OHDA-Induced Damage

The CNS/PNS Extracellular Matrix Provides Instructive Guidance Cues to Neural Cells and Neuroregulatory Proteins in Neural Development and Repair

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