Pleiotropic Effects of IGF1 on the Oocyte

Kordowitzki, Paweł; Krajnik, Kornelia; Skowrońska, Agnieszka; Skowroński, Mariusz T.

doi:10.3390/cells11101610

Cited by 5 publications

(3 citation statements)

References 43 publications

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“…Unlike FGF2, LIF acts through two signaling cascades, MAPK3/1 and the signal transducer and activator of transcription 3 (STAT3)-dependent pathways, but is more heavily controlled by JAK/STAT3 [ 8 ]. Similarly, IGF1 acts on both the oocyte as well as the embryo, increasing oocyte quality and embryonic development through the phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) pathway [ 9 , 10 ].…”

Section: Discussionmentioning

confidence: 99%

“…Each cytokine has beneficial effects on in vitro maturation that are amplified when the cytokines are added in combination. FGF2 increases cumulus cell expansion [ 7 ], LIF increases oocyte maturation and early embryonic development [ 8 ], and IGF1 increases oocyte quality and embryonic development [ 9 , 10 ]. Researchers investigated the effects of maturation medium supplementation with these three cytokines, termed FLI, in the porcine model.…”

Section: Introductionmentioning

confidence: 99%

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Cytokine-Supplemented Maturation Medium Enhances Cytoplasmic and Nuclear Maturation in Bovine Oocytes

Blocher,

Liu,

Patrick

et al. 2024

Animals

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Bovine in vitro oocyte maturation (IVM) is an easy way to obtain oocytes for subsequent assisted reproductive techniques but is inefficient compared to in vivo maturation. Supplementation of three cytokines, fibroblast growth factor 2 (FGF2), leukemia inhibitory factor (LIF), and insulin-like growth factor 1 (IGF1), or FLI, has increased oocyte maturation and embryo development in multiple species, but studies have not explored the oocyte differences caused by FLI IVM supplementation. This study aimed to assess important nuclear and cytoplasmic maturation events in high-quality oocytes. FLI-supplemented oocytes had a decreased GV (3.0% vs. 13.7%, p < 0.01) and increased telophase I incidence (34.6% vs. 17.6%, p < 0.05) after IVM, increased normal meiotic spindles (68.8% vs. 50.0%, p < 0.001), and an increased nuclear maturation rate (75.1% vs. 66.8%, p < 0.001). Moreover, in metaphase II oocytes, the percentage of FLI-treated oocytes with a diffuse mitochondrial distribution was higher (87.7% vs. 77.5%, p < 0.05) and with a cortical mitochondrial distribution was lower (11.6% vs. 17.4%, p < 0.05). Additionally, FLI-supplemented oocytes had more pattern I cortical granules (21.3% vs. 14.4%, p < 0.05). These data suggest that FLI supplementation in bovine in vitro maturation medium coordinates nuclear and cytoplasmic maturation to produce higher-quality oocytes.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cytokine-Supplemented Maturation Medium Enhances Cytoplasmic and Nuclear Maturation in Bovine Oocytes

Blocher,

Liu,

Patrick

et al. 2024

Animals

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“…The vast majority of PGCs and primary oocytes undergo caspase-dependent apoptosis [ 68 , 69 ]. The survival and proliferation of PGCs are crucially dependent on external signaling molecules such as KIT ligand (KITLG (SCF)), Leukemia inhibitory factor (LIF), Insulin like growth factor 1 (IGF1), retinoic acid, and cAMP activators [ 70 , 71 ]. The presence of KITLG and LIF is essential to prevent PGC apoptosis in vitro [ 60 ], and both factors are secreted by pre-granulosa and granulosa cells, promoting PGC survival in vivo [ 61 , 62 ].…”

Section: Regulation Of Pcd During Embryonic and Juvenile Developmentmentioning

confidence: 99%

A matter of new life and cell death: programmed cell death in the mammalian ovary

Chesnokov,

Mamedova,

Zhivotovsky

et al. 2024

J Biomed Sci

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Background The mammalian ovary is a unique organ that displays a distinctive feature of cyclic changes throughout the entire reproductive period. The estrous/menstrual cycles are associated with drastic functional and morphological rearrangements of ovarian tissue, including follicular development and degeneration, and the formation and subsequent atrophy of the corpus luteum. The flawless execution of these reiterative processes is impossible without the involvement of programmed cell death (PCD). Main text PCD is crucial for efficient and careful clearance of excessive, depleted, or obsolete ovarian structures for ovarian cycling. Moreover, PCD facilitates selection of high-quality oocytes and formation of the ovarian reserve during embryonic and juvenile development. Disruption of PCD regulation can heavily impact the ovarian functions and is associated with various pathologies, from a moderate decrease in fertility to severe hormonal disturbance, complete loss of reproductive function, and tumorigenesis. This comprehensive review aims to provide updated information on the role of PCD in various processes occurring in normal and pathologic ovaries. Three major events of PCD in the ovary—progenitor germ cell depletion, follicular atresia, and corpus luteum degradation—are described, alongside the detailed information on molecular regulation of these processes, highlighting the contribution of apoptosis, autophagy, necroptosis, and ferroptosis. Ultimately, the current knowledge of PCD aberrations associated with pathologies, such as polycystic ovarian syndrome, premature ovarian insufficiency, and tumors of ovarian origin, is outlined. Conclusion PCD is an essential element in ovarian development, functions and pathologies. A thorough understanding of molecular mechanisms regulating PCD events is required for future advances in the diagnosis and management of various disorders of the ovary and the female reproductive system in general.

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Molecular characteristics and regulatory role of insulin-like growth factor 1 gene in testicular Leydig cells of Tibetan sheep

Wu,

Li,

et al. 2024

Sci Rep

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This study aimed to analyze the molecular characteristics of insulin-like growth factor 1 ( IGF1 ) gene in the testes of Tibetan sheep and its role in the testosterone synthesis and cell development. First, we cloned IGF1 gene for bioinformatics analysis, and the primary Leydig cells (LCs) of Tibetan sheep were isolated to explore its effect on the proliferation, apoptosis and function of LCs. Finally, the specific regulatory mechanism of IGF1 on LCs was analyzed by transcriptome sequencing. Results showed that overexpression of IGF1 increased the proliferation rate and decreased apoptosis of LCs. In addition, overexpression of IGF1 altered expression of genes related to testosterone synthesis and transformation and significantly increased amount of the final product testosterone. Mechanistically, IGF1 stimulated the expression of the proliferating cell nuclear antigen and IGF1R and promoted the proliferation of LCs via the PI3K/Akt signaling pathway. Collectively, what should be clear from the results reported here is that IGF1 might play roles in the proliferation or differentiation and testosterone synthesis of LCs. These findings add to our understanding on the regulation of testosterone synthesis in sheep and other mammals. Supplementary Information The online version contains supplementary material available at 10.1038/s41598-024-75234-2.

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Pleiotropic Effects of IGF1 on the Oocyte

Cited by 5 publications

References 43 publications

Cytokine-Supplemented Maturation Medium Enhances Cytoplasmic and Nuclear Maturation in Bovine Oocytes

Cytokine-Supplemented Maturation Medium Enhances Cytoplasmic and Nuclear Maturation in Bovine Oocytes

A matter of new life and cell death: programmed cell death in the mammalian ovary

Molecular characteristics and regulatory role of insulin-like growth factor 1 gene in testicular Leydig cells of Tibetan sheep

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