Pleiotropic Functions of Cytochrome P450 Monooxygenase-Derived Eicosanoids in Cancer

Luo, Ying; Liu, Jun-Yan

doi:10.3389/fphar.2020.580897

Cited by 15 publications

(5 citation statements)

References 172 publications

(175 reference statements)

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“…Additionally, a number of enzymes' activity can be regulated by certain DCGs, including monooxygenase activity, steroid hydroxylase activity, oxidoreductase activity, and arachidonic acid monooxygenase. These enzymes have been shown to mediate the occurrence and progression of certain malignant tumors, including HCC, through the regulation of lipid metabolism [36,37].In conclusion, the relevant enzyme activities, aberrant gene expression, and an increase or reduction in metabolites may affect the biological behavior of HCC cells by regulating lipid synthesis and catabolism, and consequently, the biological behavior of HCC cells. According to our ndings, PLIN1-related DCGs have a wide range of biological activities that are intimately connected to the metabolism of lipids and organic acids.…”

Section: Discussionmentioning

confidence: 99%

Clinical Pathological Significance and Biological Function of PLIN1 in Hepatocellular Carcinoma: bioinformatics analysis and in vitro experiment

Huang,

Wei,

et al. 2024

Preprint

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Background & aims: Perilipin1 (PLIN1) is an essential lipid droplet surface protein that participates in cell life activities by regulating energy balance and lipid metabolism. PLIN1 has been shown to have a close relationship with the development of numerous tumor types. The purpose of this work is to elucidate the clinicopathologic significance of PLIN1 in hepatocellular carcinoma (HCC), as well as its impact on the biological functions of HCC cells, and investigate possible mechanisms. Methods: Public high-throughput RNA microarray and RNA sequencingwere collected to examine PLIN1 levels and clinical significance in HCC. Immunohistochemistry (IHC) and quantitative real-time polymerase chain reaction (RT-qPCR) were conducted to assess PLIN1 expression levels and clinicopathological relevance of PLIN1 in HCC. Then, SK and Huh7 cells were transfected with a lentivirus overexpressed PLIN1. CCK8 assay, wound healing assay, transwell assay, and flow cytometric analysis were conducted to explore the effects of PLIN1 overexpression on HCC cell proliferation, migration, invasion, and cell cycle distribution. Ultimately, Gene Ontology (GO) functional annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed to investigate the underlying mechanisms of PLIN1 in HCC base on HCC differentially expressed genes and PLIN1 co-expressed genes. Results: PLIN1 was markedly down-regulated in HCC tissues, which correlated with a noticeably worse prognosis for HCC patients. Additionally, PLIN1 overexpression inhibited the proliferation, migration, and invasion in SK and Huh7 cells in vitro, as well as arresting the HCC cell cycle at the G0/G1 phase. More significantly, energy conversion-related biological processes, lipid metabolism, and cell cycle signaling pathways were the three most concentrated molecular mechanisms. Conclusion: The current study found that down-regulated PLIN1 is associated with a poor prognosis in HCC patients and prevented HCC progression by inhibiting cellular proliferation, migration, and metastasis, as well as the mechanisms underlying the regulation of lipid metabolism-related pathways in HCC.

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Section: Discussionmentioning

confidence: 99%

Clinical Pathological Significance and Biological Function of PLIN1 in Hepatocellular Carcinoma: bioinformatics analysis and in vitro experiment

Huang,

Wei,

et al. 2024

Preprint

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“…CYP genes have been extensively confirmed to be involved in the metabolism of procarcinogenic compounds to highly carcinogenic metabolites [ 29 , 30 ]. Other studies have shown that the genes encoding these proteins are linked to cell signalling pathways critical for cell cycle regulation [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

Comparative Studies on a Standardized Subfraction of Red Onion Peel Ethanolic Extract (Plant Substance), Quercetin (Pure Compound), and Their Cell Mechanism and Metabolism on MDA-MB-231

Leong

Chao

Siah

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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This study indicates the presence of quercetin in subfraction F1 and the standardized value of F1 derived from research using ultra-performance liquid chromatography (UPLC) and AlCl3 colorimetric assays, which further proved that both F1 and quercetin are potential growth inhibitors in MDA-MB-231 cells by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. In the process, staining of F1-treated cells with annexin/propidium iodide (PI) reduced cell proliferation and induced only S and G2 phases of cell cycle arrest in the treated cells by flow cytometry. Quercetin reduced cell proliferation by inducing apoptosis and S phase arrest. The 5′-bromo-2′-deoxyuridine (BrdU) incorporation of DNA synthesis in MDA-MB-231 cells was also inhibited after F1 and quercetin treatments. F1 and quercetin induced CYP1A1 and CYP1B1 gene expression, but only F1 induced CYP2S1 gene expression in the treated cells. Both F1 and quercetin inhibited the proliferation of MDA-MB-231 cells in different ways, but F1 is likely a better potential anticancer agent derived from the green approach towards breast cancer treatment.

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“…They use a broad range of structurally diverse substrates and form their products with high stereo- and regio-selectivity. Humans carry 57 different P450 (CYP) enzymes involved in the metabolism of steroid hormones, other sterols, vitamin D 3 , eicosanoids, fatty acids, and retinoic acid (Luo and Liu 2020 ; Rendic and Guengerich 2021 ; Sarparast et al 2020 ). They catalyze almost exclusively monooxygenase reactions through the activation of molecular oxygen using a single electron.…”

Section: Production Of Biotechnological Important Proteins: Multiple Cytochrome P450 Suitable For Whole-cell Transformationsmentioning

confidence: 99%

The “beauty in the beast”—the multiple uses of Priestia megaterium in biotechnology

Biedendieck

Knuuti

Jahn

2021

Appl Microbiol Biotechnol

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Over 30 years, the Gram-positive bacterium Priestia megaterium (previously known as Bacillus megaterium) was systematically developed for biotechnological applications ranging from the production of small molecules like vitamin B12, over polymers like polyhydroxybutyrate (PHB) up to the in vivo and in vitro synthesis of multiple proteins and finally whole-cell applications. Here we describe the use of the natural vitamin B12 (cobalamin) producer P. megaterium for the elucidation of the biosynthetic pathway and the subsequent systematic knowledge-based development for production purposes. The formation of PHB, a natural product of P. megaterium and potential petro-plastic substitute, is covered and discussed. Further important biotechnological characteristics of P. megaterium for recombinant protein production including high protein secretion capacity and simple cultivation on value-added carbon sources are outlined. This includes the advanced system with almost 30 commercially available expression vectors for the intracellular and extracellular production of recombinant proteins at the g/L scale. We also revealed a novel P. megaterium transcription-translation system as a complementary and versatile biotechnological tool kit. As an impressive biotechnology application, the formation of various cytochrome P450 is also critically highlighted. Finally, whole cellular applications in plant protection are completing the overall picture of P. megaterium as a versatile giant cell factory. Key points • The use of Priestia megaterium for the biosynthesis of small molecules and recombinant proteins through to whole-cell applications is reviewed. • P. megaterium can act as a promising alternative host in biotechnological production processes.

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Pleiotropic Functions of Cytochrome P450 Monooxygenase-Derived Eicosanoids in Cancer

Cited by 15 publications

References 172 publications

Clinical Pathological Significance and Biological Function of PLIN1 in Hepatocellular Carcinoma: bioinformatics analysis and in vitro experiment

Clinical Pathological Significance and Biological Function of PLIN1 in Hepatocellular Carcinoma: bioinformatics analysis and in vitro experiment

Comparative Studies on a Standardized Subfraction of Red Onion Peel Ethanolic Extract (Plant Substance), Quercetin (Pure Compound), and Their Cell Mechanism and Metabolism on MDA-MB-231

The “beauty in the beast”—the multiple uses of Priestia megaterium in biotechnology

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