Plethysmography for the assessment of pneumococcal pneumonia and passive immunotherapy in a mouse model

Hennezel, Laetitia De; Debarre, S.; Ramisse, Françoise; Delamanche, S.; Harf, Alain; Alonso, Jean-Michel; Calvet, J

doi:10.1183/09031936.01.17100940

Cited by 14 publications

(13 citation statements)

References 16 publications

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“…Spontaneous ventilation, measured in mice using plethysmography (e.g., using the Buxco system), appears to be disparately affected by lung infection in mice and in humans. Whereas human patients with pneumonia typically exhibit rapid shallow breathing, mice instead develop a breathing pattern with a decreased respiratory rate and little or no change in tidal volume, resulting in decreased minute ventilation (20,75). Changes in lung mechanics in pneumonic mice can be measured using a ventilator (e.g., with the flexiVent system) and are generally consistent with those observed in human patients.…”

Section: The Next Practical Considerations: How To Get Information Outmentioning

confidence: 59%

Animal models of human pneumonia

Mizgerd¹,

Skerrett

2008

American Journal of Physiology-Lung Cellular and Molecular Phys

127

118

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Pneumonia is a medical and public health priority, and advances against this disease will require improved knowledge of biological mechanisms. Human pneumonia is modeled with experimental infections of animals, most frequently mice. Mouse models are leading to important discoveries relevant to pneumonia, but their limitations must be carefully considered. Several approaches to establishing pneumonia in mice have been developed, and each has specific strengths and weaknesses. Similarly, procedures for characterizing microbial and host responses to infection have unique advantages and disadvantages. Mice are not small humans, and the applicability of results from murine models to human disease depends on understanding the similarities and differences between species. Additional considerations such as mouse strain, microbe strain, and prior mouse-microbe interactions also influence the design and interpretation of experiments. Results from studies of pneumonia in animals, combined with complementary basic and translational studies, are elucidating mechanisms responsible for susceptibility to and pathophysiology of lung infection.

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Section: The Next Practical Considerations: How To Get Information Outmentioning

confidence: 59%

Animal models of human pneumonia

Mizgerd¹,

Skerrett

2008

American Journal of Physiology-Lung Cellular and Molecular Phys

127

118

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“…coli pneumonia decreased the respiratory rate of mice without affecting tidal volume. Pneumococcal pneumonia induces similar ventilatory changes in mice (8). These ventilatory changes during E. coli or S. pneumoniae pneumonia in mice decrease minute ventilation to about half the levels of nonpneumonic mice.…”

Section: Discussionmentioning

confidence: 74%

“…Inflammation, including neutrophil emigration and plasma extravasation, can impair lung function (25,65). In rodents with bacterial pneumonia, the severity of infection associates with increased inflammation, decreased minute ventilation, and decreased lung compliance (8,48). Therefore, we assessed whether these cytokine receptors contributed to changes in ventilation or compliance during pneumonia.…”

Section: Generation Of Tm Mice Unresponsive To Tnf or Il-1mentioning

confidence: 99%

Roles for early response cytokines duringEscherichia colipneumonia revealed by mice with combined deficiencies of all signaling receptors for TNF and IL-1

Mizgerd¹,

Lupa²,

Hjoberg³

et al. 2004

American Journal of Physiology-Lung Cellular and Molecular Phys

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“…42 Therapeutic intervention with DEX reversed this effect and increased MV in mice. It has been noted that high tidal volumes and minute ventilations antagonize the development of Mch induced bronchoconstriction in mice and humans.…”

Section: Vaickus Et Almentioning

confidence: 99%

Assessing Pulmonary Pathology by Detailed Examination of Respiratory Function

Vaickus

Bouchard

Kim

et al. 2010

The American Journal of Pathology

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Pulmonary inflammation causes multiple alterations within the lung , including mucus production , recruitment of inflammatory cells , and airway hyperreactivity (AHR). Measurement of AHR by direct , invasive means (eg , mechanical ventilation)or noninvasive techniques , like whole body plethysmography (WBP) , assesses the severity of pulmonary inflammation in animal models of inflammatory lung disease. Direct measurement of AHR is acknowledged as the most accurate method for assessing airway mechanics , but analysis of all data obtained from WBP may offer insights into which inflammatory aspects of the lung are altered along with AHR. Using WBP , we compared the respiratory parameters of two groups of mice sensitized with cockroach allergen. One group was treated with dexamethasone (Dex) before final challenge (DexAsthma) , while the other group received vehicle treatment (Asthma). Respiratory parameters from plethysmography revealed that Dex-Asthma mice compensated to maintain high minute ventilation , whereas Asthma mice showed significant impairment in minute ventilation despite increased peak expiratory flow (103 ؎ 5 ml/min vs. 69 ؎ 70 ml/ min). The WBP data suggest that enhanced air exchange in the Dex-Asthma mice results from significant decreases in airway mucus production. Additional studies with quantitative morphometry of histological sections confirmed that Dex reduced airway mucus. In conclusion , a detailed examination of WBP parameters can accurately assess the respiratory health of mice and will help direct additional studies. Numerous relevant animal models of disease have advanced the understanding of biology and provided important insights into the pathogenesis of human disease. The vast majority of these studies have been carried out in mice, due to their small size and the extensive characterization of the murine inflammatory system. Several robust murine models of allergic asthma have been developed in recent years, 1 including many experimental systems which induce allergic sensitization to ovalbumin (Ova). Although this system allows close control over the nature of the sensitizing agent, Ova is a relatively poor allergen and requires adjuvant co-administration or relatively high and frequent doses to achieve satisfactory immunization.2-5 Our model, conversely, uses an allergen composed of the defatted, total body extract of German cockroaches (cockroach allergen, CRA) containing appreciable lipopolysaccharide. CRA is a highly potent allergen (robust immunization is achieved with a dose thousands of times less than that required for Ova) and does not require an adjuvant for sensitization.6,7 Campbell et al 7 established the first CRA induced model of asthma in mice and demonstrated robust pulmonary inflammation with many asthma-like features. The complex nature of this allergen and its close correspondence with its urban environmental counterpart makes this model highly applicable to the study of human disease.In most models of allergic airways inflammation, disease severity and or therape...

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Plethysmography for the assessment of pneumococcal pneumonia and passive immunotherapy in a mouse model

Cited by 14 publications

References 16 publications

Animal models of human pneumonia

Animal models of human pneumonia

Roles for early response cytokines duringEscherichia colipneumonia revealed by mice with combined deficiencies of all signaling receptors for TNF and IL-1

Assessing Pulmonary Pathology by Detailed Examination of Respiratory Function

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