Pleural lavage with distilled water during surgery for esophageal squamous cell carcinoma

Kosuga, Toshiyuki; Shiozaki, Atsushi; Ichikawa, Daisuke; Fujiwara, Hitoshi; Komatsu, Shuhei; Iitaka, Daisuke; Tsujiura, Masahiro; Morimura, Ryo; Takeshita, Hitoshi; Nagata, Hiroaki; Okamoto, Kazuma; Nakahari, Takashi; Marunaka, Yoshinori; Otsuji, Eigo

doi:10.3892/or.2011.1307

Cited by 12 publications

(8 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, we reincubated these cells after they had been exposed to DW and found that the decrease observed in the number of cells in each of the three gastric cancer cell lines depended on the time for which they had been exposed to DW. Similar findings have been reported in esophageal and pancreatic cancer cells [17, 18]. These results suggest that hypotonic shock could be applied for the treatment of dissemination from gastric cancer by using a peritoneal injection of hypotonic solution.…”

Section: Discussionsupporting

confidence: 88%

“…RVD was shown to occur after hypotonicity-induced cellular swelling and has been attributed to the activation of ion channels and transporters, which cause K + , Cl − , and H 2 O effluxes, ultimately leading to cell shrinkage [26, 27]. Similar phenomena have been reported in esophageal and pancreatic cancer cells [17, 18]. We could not use NPPB, which blocks multiple types of chloride channels, in the present study because of its neurotoxicity in vivo .…”

Section: Discussionmentioning

confidence: 80%

See 1 more Smart Citation

Efficacy of a Hypotonic Treatment for Peritoneal Dissemination from Gastric Cancer Cells: AnIn VivoEvaluation

Shiozaki

Ichikawa

Takemoto

et al. 2014

BioMed Research International

Self Cite

View full text Add to dashboard Cite

The aim of the present study was to determine the efficacy of a hypotonic treatment for peritoneal dissemination from gastric cancer cells using an in vivo model. We firstly evaluated the toxicity of a peritoneal injection of distilled water (DW) (2 mL for 3 days) in mice. Macroscopic and microscopic examinations revealed that the peritoneal injection of DW did not severely damage the abdominal organs of these mice. MKN45 gastric cancer cells preincubated with NaCl buffer or DW for 20 minutes in vitro were then intraperitoneally injected into nude mice, and the development of dissemination nodules was analyzed. The total number, weight, and volume of the dissemination nodules were significantly decreased by the DW preincubation. We then determined whether the peritoneal injection of DW inhibited the establishment of peritoneal dissemination. After a peritoneal injection of MKN45 cells into nude mice, NaCl buffer or DW was injected into the abdominal cavity for 3 days. The total volume of dissemination nodules was significantly lower in DW-injected mice than in NaCl-injected mice. In conclusion, we demonstrated the safeness of a peritoneal injection of DW. Furthermore, the development of dissemination nodules from gastric cancer cells was prevented by a preincubation with or peritoneal injection of DW.

show abstract

Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 80%

Efficacy of a Hypotonic Treatment for Peritoneal Dissemination from Gastric Cancer Cells: AnIn VivoEvaluation

Shiozaki

Ichikawa

Takemoto

et al. 2014

BioMed Research International

Self Cite

View full text Add to dashboard Cite

show abstract

“…The efficacy of pleural lavage with distilled water during surgery for esophageal squamous cell carcinoma was proposed from the cytocidal effects of hypotonic shock on tumor cells [27]. It is also used as an alternative agent for wound cleansing.…”

Section: Discussionmentioning

confidence: 99%

The pH of water from various sources: an overview for recommendation for patients with atopic dermatitis

Kulthanan

Nuchkull

Varothai

2013

Asia Pacific Allergy

View full text Add to dashboard Cite

BackgroundPatients with atopic dermatitis (AD) have increased susceptibility to irritants. Some patients have questions about types of water for bathing or skin cleansing.ObjectiveWe studied the pH of water from various sources to give an overview for physicians to recommend patients with AD.MethodsWater from various sources was collected for measurement of the pH using a pH meter and pH-indicator strips.ResultsBottled drinking still water had pH between 6.9 and 7.5 while the sparkling type had pH between 4.9 and 5.5. Water derived from home water filters had an approximate pH of 7.5 as same as tap water. Swimming pool water had had pH between 7.2 and 7.5 while seawater had a pH of 8. Normal saline and distilled water had pH of 5.4 and 5.7, respectively. Facial mineral water had pH between 7.5 and 8, while facial makeup removing water had an acidic pH.ConclusionNormal saline, distilled water, bottled sparkling water and facial makeup removing water had similar pH to that of normal skin of normal people. However, other factors including benefits of mineral substances in the water in terms of bacteriostatic and anti-inflammation should be considered in the selection of cleansing water.

show abstract

“…The supernatant was then discarded, isotonic NaCl solution was added to the pelleted cells, and the suspended cells were mounted on a coverslip that had been pre-coated with neutralized Cell-Tak (Becton-Dickinson Labware, Bedford, MA, USA) in order to retain them firmly. The coverslip was then placed in a perfusion chamber that had been mounted on the stage of a DIC microscope (ECLIPSE Ti, Nikon, Japan) connected to a video-enhanced contrast (VEC) system (ARGUS-20, Hamamatsu Photonics, Hamamatsu, Japan), and images were continuously recorded by a video recorder, according to a previously described procedure 9 - 12 . Experiments were carried out at room temperature (20-24ºC).…”

Section: Methodsmentioning

confidence: 99%

“…This lavage procedure has been performed on digestive cancer patients, and has cytocidal effects on exfoliated viable cancer cells, thereby preventing peritoneal recurrence. We previously demonstrated the cytocidal effects of this therapy on esophageal, gastric, pancreatic and colorectal cancers in vitro and in vivo 9 - 14 . The efficacy of this therapy for HCC was reported previously, and has been employed during surgery 15 - 16 .…”

Section: Introductionmentioning

confidence: 97%

Inhibition of Regulatory Volume Decrease Enhances the Cytocidal Effect of Hypotonic Shock in Hepatocellular Carcinoma

Kudou¹,

Shiozaki²,

Kosuga³

et al. 2016

J. Cancer

Self Cite

View full text Add to dashboard Cite

Background: Hypotonic shock induces cytocidal effects through cell rupture, and cancer therapy based on this mechanism has been clinically administered to hepatocellular carcinoma patients. We herein investigated the effectiveness of hypotonic shock combined with the inhibition of regulatory volume decrease as cancer therapy for hepatocellular carcinoma.Methods: Morphological changes in human hepatocellular carcinoma cell lines were observed under a differential interference contrast microscope connected to a high-speed digital video camera. Cell volume changes under hypotonic shock with or without chloride, potassium, or water channel blockers were observed using a high-resolution flow cytometer. In order to investigate cytocidal effects, the number of surviving cells was compared after exposure to hypotonic solution with and without each channel blocker (re-incubation experiment).Results: Video recordings showed that cells exposed to distilled water rapidly swelled and then ruptured. Cell volume measurements revealed regulatory volume decrease under mild hypotonic shock, whereas severe hypotonic shock increased the number of broken fragments as a result of cell rupture. Moreover, regulatory volume decrease was inhibited in cells treated with each channel blocker. Re-incubation experiments showed the cytocidal effects of hypotonic shock in cells exposed to hypotonic solution, and additional treatments with each channel blocker enhanced these effects.Conclusion: The inhibition of regulatory volume decrease with chloride, potassium, or water channel blockers may enhance the cytocidal effects of hypotonic shock in hepatocellular carcinoma. Hypotonic shock combined with the inhibition of regulatory volume decrease was a more effective therapy than hypotonic shock alone.

show abstract

Pleural lavage with distilled water during surgery for esophageal squamous cell carcinoma

Cited by 12 publications

References 26 publications

Efficacy of a Hypotonic Treatment for Peritoneal Dissemination from Gastric Cancer Cells: AnIn VivoEvaluation

Efficacy of a Hypotonic Treatment for Peritoneal Dissemination from Gastric Cancer Cells: AnIn VivoEvaluation

The pH of water from various sources: an overview for recommendation for patients with atopic dermatitis

Inhibition of Regulatory Volume Decrease Enhances the Cytocidal Effect of Hypotonic Shock in Hepatocellular Carcinoma

Contact Info

Product

Resources

About