PLL-alginate and the HPMC-EC hybrid coating over the 3D DNA nanocubes as compact nanoparticles for oral administration

Sohail, Muhammad; Mirjat, Ali Asghar; Naveed, Muhammad; Majeed, Fatima; Raza, Faisal; Farooq, Muhammad Asim; Mikrani, Reyaj; Khan, Salman; Abbas, Muhammad; Ullah, Sana; Hasnat, Muhammad; Wen, Chunxia; Khan, Ghulam Jilany; Ansari, Muhammad Tayyab

doi:10.1007/s13204-019-01075-5

Cited by 16 publications

(10 citation statements)

References 32 publications

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“…The loading efficiency was observed to be 97.35% in the case of 0.1 nM CPT (C1 formulation) to 66.85% in the case of 51.2 nM (C10 formulation), at room temperature and physiological pH (pH 7.45). This finding might be due to the limited availability of binding sites and exposed pockets for CPT binding provided by consecutive G and C nucleotides, especially near the major and minor grooves of the double helix [ 19 ].…”

Section: Resultsmentioning

confidence: 99%

“…Different constituting DNA strands corresponding to the final self-assembly of DNA-NWs should not separate or segregate upon applied voltage and current (75 V, 20 A). This finding served as an indication of the integrity and strength of the self-assembly of DNA strands and the firm structure of the DNA-NW [ [17] , [18] , [19] ].…”

Section: Methodsmentioning

confidence: 99%

“…Each triangle tile is designed to contain sticky ends at the vertices to combine (with each other), making a compact polymer, resulting in DNA-nanosheets that fold and spin onto itself due to the helical structure and curvature of the DNA to yield compact DNA-NWs [ 17 , 18 ]. After tagging CPT with the fluorescein isothiocyanate (FITC) dye, the loading of CPT/FITC onto the DNA-NWs was confirmed by confocal microscopy and UV analysis [ 19 ]. The mechanism of targeting and internalization of the CPT-loaded DNA-NWs is illustrated in Fig.…”

Section: Introductionmentioning

confidence: 99%

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The effective transfection of a low dose of negatively charged drug-loaded DNA-nanocarriers into cancer cells via scavenger receptors

Chengfei¹,

Akhtar²,

Ammara³

et al. 2021

Journal of Pharmaceutical Analysis

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DNA-nanotechnology-based nano-architecture scaffolds based on circular strands were designed in the form of DNA-nanowires (DNA-NWs) as a polymer of DNA-triangles. Circularizing a scaffold strand (84-NT) was the critical step followed by annealing with various staple strands to make stiff DNA-triangles. Atomic force microcopy (AFM), native polyacrylamide gel electrophoresis (PAGE), UV-analysis, MTT-assay, flow cytometry, and confocal imaging were performed to assess the formulated DNA-NWs and cisplatin (CPT) loading. The AFM and confocal microscopy images revealed a uniform shape and size distribution of the DNA-NWs, with lengths ranging from 2 to 4 μm and diameters ranging from 150 to 300 nm. One sharp band at the top of the lane (500 bp level) with the loss of electrophoretic mobility during the PAGE (native) gel analysis revealed the successful fabrication of DNA-NWs. The loading efficiency of CPT ranged from 66.85% to 97.35%. MTT and flow cytometry results showed biocompatibility of the blank DNA-NWs even at 95% concentration compared with the CPT-loaded DNA-NWs. The CPT-loaded DNA-NWs exhibited enhanced apoptosis (22%) compared to the apoptosis (7%) induced by the blank DNA-NWs. The release of CPT from the DNA-NWs was sustained at < 75% for 6 h in the presence of serum, demonstrating suitability for systemic applications. The IC 50 of CPT@DNA-NWs was reduced to 12.8 nM CPT, as compared with the free CPT solution exhibiting an IC 50 of 51.2 nM. Confocal imaging revealed the targetability, surface binding, and slow internalization of the DNA-NWs in the scavenger-receptor-rich cancer cell line (HepG2) compared with the control cell line.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The effective transfection of a low dose of negatively charged drug-loaded DNA-nanocarriers into cancer cells via scavenger receptors

Chengfei¹,

Akhtar²,

Ammara³

et al. 2021

Journal of Pharmaceutical Analysis

Self Cite

View full text Add to dashboard Cite

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“…This was then shown to be attributed to the sustained inhibition of dipeptidyl peptidase‐4 (DPP‐4) in the intestine and blood, causing elevation of the levels of incretin peptides, including glucagon‐like peptide 1 (GLP1) and glucose‐dependent insulinotropic polypeptide (GIP) 42,43 . Hybrid nanoparticles fabricated from conjugating multiple polymer coatings over DNA‐nanocubes showed prolonged vildagliptin release and superior control of glycemic levels than the control group of diabetic mice 44,45 . One study demonstrated the intercalation of antitumor drugs (containing heavy metals for example, cisplatin, carboplatin, etc.)…”

Section: Supramolecular Approach To Synthesize Dna‐based Nanostructuresmentioning

confidence: 99%

“…CpG motifs (unmethylated‐cytosine phosphate guanosine stretches) functionalized to DNA nanostructures (Y‐shaped or tetrahedral shaped) caused immuno‐stimulation through the activation of Toll‐like/receptor 9 (TLR9) in macrophages 60 . However, dose‐dependent liver/kidney elimination, minimum effective or toxic dose determinations remain to be evaluated after systemic administration of DNA nanostructures 44 . CpG functionalized to DNA‐nanostructures was further tagged with the antigen, and these acted like antigen‐presenting cells for the immunological responses to generate specific antibodies in the body.…”

Section: Compact 3d Dna Nano‐assemblies For In Vitro and Systemic Drumentioning

confidence: 99%

Development and functionalization of DNA nanostructures for biomedical applications

Zou

Neelakantan

Zhang

2021

J Chinese Chemical Soc

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Nanomaterials are excellent drug delivery systems, yet, they must be functionalized in a manner compatible with the biological environment. Regarding delivery of the payloads, it is critical to monitor the nanocarrier's biocompatibility and the ability to control its drug encapsulation and release, as well as targeting. The current challenges include avoiding negative host immune responses, optimizing stability in biological environments, and achieving precise interactions with the targets. Contemporary advances in structural DNA nanotechnology, DNA origami, and supramolecular DNA assembly make it possible to produce complex multi‐functional DNA nanostructures, wherein precise control of the size, geometry, and appearance of the ligands is feasible. DNA nanostructures offer ease of synthesis and conjugation of functional moieties to target the release of cargo or to analyze important biomarkers for diagnostics. Furthermore, the biocompatibility, programmability, responsiveness to biomolecules, cell‐surfaces, and organisms make such DNA nanomaterials highly suitable for potential translational applications. This overview summarizes the recent developments in functionalizing, stabilizing, and applying DNA nanostructures for potential biomedical applications.

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Synthesis of Ligand Functionalized ErbB-3 Targeted Novel DNA Nano-Threads Loaded with the Low Dose of Doxorubicin for Efficient In Vitro Evaluation of the Resistant Anti-Cancer Activity

et al. 2020

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PLL-alginate and the HPMC-EC hybrid coating over the 3D DNA nanocubes as compact nanoparticles for oral administration

Cited by 16 publications

References 32 publications

The effective transfection of a low dose of negatively charged drug-loaded DNA-nanocarriers into cancer cells via scavenger receptors

The effective transfection of a low dose of negatively charged drug-loaded DNA-nanocarriers into cancer cells via scavenger receptors

Development and functionalization of DNA nanostructures for biomedical applications

Synthesis of Ligand Functionalized ErbB-3 Targeted Novel DNA Nano-Threads Loaded with the Low Dose of Doxorubicin for Efficient In Vitro Evaluation of the Resistant Anti-Cancer Activity

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