PLLA scaffolds produced by thermally induced phase separation (TIPS) allow human chondrocyte growth and extracellular matrix formation dependent on pore size

Conoscenti, Gioacchino; Schneider, Tobias; Stoelzel, Katharina; Pavia, Francesco Carfì; Brucato, V.; Goegele, C; Carrubba, Vincenzo La; Schulze‐Tanzil, Gundula

doi:10.1016/j.msec.2017.06.011

Cited by 84 publications

(57 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, the presence of subchondral cysts was only observed in PLLA+CHT. A possible explanation would be that biomaterials were reabsorbed at a similar rate, but whereas PLLA was found as microspheres, CHT would have been dragged into these cysts in the subchondral bone, probably originating from an immune response already described in the literature with biomaterials (Chu et al, ; Conoscenti et al, ), although our in vitro experiments only showed an increase in NO production in the PLLA+CHT group after culturing for 24 hr.…”

Section: Discussionmentioning

confidence: 71%

“…These scaffolds should be non‐immunogenic and biodegradable to avoid deposits, porous to allow migration and adhesion of cells from subchondral bone, and mechanically stable to sustain the regeneration process. Bioresorbable polyesters such as polylactide (PLA) (Chu et al, ; Conoscenti et al, ) or polycaprolactone (PCL) (Martinez‐Diaz et al, ; Vikingsson et al, ) have been used previously in cartilage engineering animal models. Cell‐free strategies using PCL (Martinez‐Diaz et al, ; Vikingsson et al, ) or biostable acrylic scaffolds of varying stiffness (Sancho‐Tello et al, ; Sancho‐Tello et al, ) probed in rabbit knee models their capacity to induce the formation of histologically high‐quality tissue with the characteristics of hyaline cartilage.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A cell‐free approach with a supporting biomaterial in the form of dispersed microspheres induces hyaline cartilage formation in a rabbit knee model

et al. 2019

View full text Add to dashboard Cite

The objective of this study was to test a regenerative medicine strategy for the regeneration of articular cartilage. This approach combines microfracture of the subchondral bone with the implant at the site of the cartilage defect of a supporting biomaterial in the form of microspheres aimed at creating an adequate biomechanical environment for the differentiation of the mesenchymal stem cells that migrate from the bone marrow. The possible inflammatory response to these biomaterials was previously studied by means of the culture of RAW264.7 macrophages. The microspheres were implanted in a 3 mm‐diameter defect in the trochlea of the femoral condyle of New Zealand rabbits, covering them with a poly(l‐lactic acid) (PLLA) membrane manufactured by electrospinning. Experimental groups included a group where exclusively PLLA microspheres were implanted, another group where a mixture of 50/50 microspheres of PLLA (hydrophobic and rigid) and others of chitosan (a hydrogel) were used, and a third group used as a control where no material was used and only the membrane was covering the defect. The histological characteristics of the regenerated tissue have been evaluated 3 months after the operation. We found that during the regeneration process the microspheres, and the membrane covering them, are displaced by the neoformed tissue in the regeneration space toward the subchondral bone region, leaving room for the formation of a tissue with the characteristics of hyaline cartilage.

show abstract

Section: Discussionmentioning

confidence: 71%

Section: Introductionmentioning

confidence: 99%

A cell‐free approach with a supporting biomaterial in the form of dispersed microspheres induces hyaline cartilage formation in a rabbit knee model

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Yen et al ( 2009 ) fabricated nano-porous polycaprolactone scaffolds which demonstrated controlled drug release for drug delivery applications. In addition, Conoscenti et al fabricated highly porous, well defined pore sized poly( l -lactic acid) scaffolds for bone engineering applications, and demonstrated the scaffolds were able to support chondrocyte differentiation (Conoscenti et al 2017 ). An advantage to this technique is that by easily changing parameters such as polymer type, solvent/non-solvent ratio, polymer concentration, heating temperature and time, and cooling rate; porous constructs can be fabricated with specific morphologies for a particular application (Akbarzadeh and Yousefi 2014 ).…”

Section: Scaffold Fabrication Techniquesmentioning

confidence: 99%

New generation of bioreactors that advance extracellular matrix modelling and tissue engineering

et al. 2018

View full text Add to dashboard Cite

Bioreactors hold a lot of promise for tissue engineering and regenerative medicine applications. They have multiple uses including cell cultivation for therapeutic production and for in vitro organ modelling to provide a more physiologically relevant environment for cultures compared to conventional static conditions. Bioreactors are often used in combination with scaffolds as the nutrient flow can enhance oxygen and diffusion throughout the 3D constructs to prevent the formation of necrotic cores. A variety of scaffolds have been fabricated to achieve a structural architecture that mimic native extracellular matrix. Future developments of in vitro models will incorporate the ability to non-invasively monitor the cellular microenvironment to enhance the understanding of in vitro conditions. This review details current advancements in bioreactor and scaffold systems and provides insight on how in vitro models can be augmented for future biomedical applications.

show abstract

“…Compared to the NIPS method, there are fewer variables to control [21][22][23][24][25][26], and the prepared membranes have a porous skin layer and bi-continuous pores, which usually results in a high permeation flux and good mechanical properties. Crystalline polymers, such as polypropylene (PP) [28], polyethylene (PE) [29], poly(vinylidene fluroride) (PVDF) [30], poly(ethylene-co-vinyl alcohol) (EVOH) [31], ethylene-acrylic acid copolymer (EAA) [32], polyphenylene (PPS) [33], poly(ether ether ketone) (PEEK) [34], poly(oxymethylene) (POM) [35], poly(ethylene chlorotrifluoroethylene) (PECTFE) [36], poly(4-methyl-1pentene) (PMP) [37] and poly(L-lactic acid) (PLLA) [38], as well as amorphous polymers, such as poly(vinyl butyral) (PVB) [39], poly(methyl methacrylate) (PMMA) [40] and polystyrene (PS) [41], have been prepared successfully by using the TIPS process. Moreover, some researchers have focused on simulating the phase separation dynamics of membrane formation in TIPS [42,43].…”

Section: Introductionmentioning

confidence: 99%

Estimation of phase separation temperatures for polyethersulfone/solvent/non-solvent systems in RTIPS and membrane properties

Liu

Skov

2018

Journal of Membrane Science

View full text Add to dashboard Cite

Phase separation temperature estimations, based on Hansen solubility parameters for poly(ethersulfone) (PES)/solvent/non-solvent systems, were carried out to study the control of phase separation temperature in a reverse thermally induced phase separation (RTIPS) process. Four membrane-forming systems were studied, namely PES/N,N-dimethylacetamide (DMAc)/diethylene glycol (DEG), PES/DMAc/polyethylene glycol 200 (PEG200), PES/DMAc/PEG300 and PES/DMAc/PEG400. The effects of PES molecular weights, PES concentrations, PEG molecular weights and ratios of non-solvent/solvent on phase separation temperature are investigated, and the theoretical Hansen solubility parameter calculation is used to establish a prediction equation for phase separation temperature. A linear relationship between the experimental data and the difference in the 2 solubility parameters between PES and the mixed solvent was observed. When the membrane-forming temperature was higher than the cloud point, membranes with a bi-continuous structure were acquired and showed a higher pure water permeation flux than that of membranes prepared with the non-solvent induced phase separation (NIPS) process. The pure water permeation flux and the mean pore size of membranes prepared with the RTIPS process decreased in line with an increase of PES molecular weight. When the membrane formation mechanism was the RTIPS process, the mechanical properties were better than those of the corresponding membranes prepared with the NIPS process. Highlights> A group contribution estimate of phase separation temperature for RTIPS was proposed.> Phase separation temperatures were linearly dependent on Hansen solubility parameters.> PES membranes with bi-continuous structure were acquired from RTIPS process. > PES membranes prepared by RTIPS showed good permeation and mechanical properties.

show abstract

PLLA scaffolds produced by thermally induced phase separation (TIPS) allow human chondrocyte growth and extracellular matrix formation dependent on pore size

Cited by 84 publications

References 56 publications

A cell‐free approach with a supporting biomaterial in the form of dispersed microspheres induces hyaline cartilage formation in a rabbit knee model

A cell‐free approach with a supporting biomaterial in the form of dispersed microspheres induces hyaline cartilage formation in a rabbit knee model

New generation of bioreactors that advance extracellular matrix modelling and tissue engineering

Estimation of phase separation temperatures for polyethersulfone/solvent/non-solvent systems in RTIPS and membrane properties

Contact Info

Product

Resources

About