Ploidy determination of buffel grass accessions in the USDA National Plant Germplasm System collection by flow cytometry

Burson, Byron L.; Actkinson, Jo M.; Hussey, M. A.; Jessup, Russell W.

doi:10.1016/j.sajb.2011.12.003

Cited by 21 publications

(46 citation statements)

References 19 publications

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“…A stable genome size within and between populations of each ploidy level was found, falling clearly into distinct classes corresponding to the three different cytotypes (Kharrat-Souissi et al 2013). Unfortunately, Burson et al (2012) tested only one individual plant of each accession and failed to test if different ploidy levels were associated with certain populations or with mixed populations.…”

Section: Flow Cytometry For Ploidy Level Determinationmentioning

confidence: 96%

“…This contrasts with the relative frequencies found in natural Tunisian populations: 4, 11, and 82%, respectively (Kharrat-Souissi et al 2013). According to several authors (Hignight et al 1991;Visser et al 1998Visser et al , 2000Burson et al 2012), the most common chromosome number reported for C. ciliaris corresponds to the tetraploid cytotype (2n = 4x = 36). However, at least for Tunisia, representing a small portion of the native range of C. ciliaris, the hexaploid is the most frequent cytotype (Kharrat-Souissi et al 2013).…”

Section: Flow Cytometry For Ploidy Level Determinationmentioning

confidence: 99%

“…But once the sexual individuals were used in breeding programs and crosses between sexual and apomictic plants were made, knowledge of the ploidy level of the plants used in hybridisation became more important, because breeding success depends on using meiotically stable parental lines with compatible chromosome numbers (Burson et al 2012). …”

Section: Polyploidy Of Cenchrus Ciliarismentioning

confidence: 99%

“…In a recent study, Burson et al (2012) determined the DNA content of many accessions of C. ciliaris from the USDA National Plant Germplasm System by flow cytometry. This germplasm collection is dominated by tetraploids (54%), with some pentaploids (25%) and a few hexaploids (4%).…”

Section: Flow Cytometry For Ploidy Level Determinationmentioning

confidence: 99%

“…In addition to the existence of different ploidy levels in C. ciliaris, several authors (Visser et al 1998;Mnif et al 2005a;Burson et al 2012) reported frequent aneuploidy [gains (hyperaneuploidy) or loss (hypo-aneuploidy) of chromosome, in one chromosome set] resulting from unequal segregation of chromosomes. Aneuploidy has a greater effect on phenotype than polyploidy and causes developmental abnormalities, which can reduce organismal fitness in species (Birchler and Veitia 2007).…”

Section: Flow Cytometry For Ploidy Level Determinationmentioning

confidence: 99%

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The polyploid nature of Cenchrus ciliaris L. (Poaceae) has been overlooked: new insights for the conservation and invasion biology of this species – a review

et al. 2014

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Abstract. Climate change, associated with increased aridity, and high grazing pressure by livestock results in the scarcity and loss of perennial Poaceae in arid ecosystems. The species threatened by this include Cenchrus ciliaris L., a native perennial grass of the tropical and sub-tropical arid rangelands of Africa and Western Asia and now introduced in Central and South America, and Australia. This species reproduces predominantly through aposporous apomixis although sexual individuals have been occasionally identified. Cenchrus ciliaris is characterised by a significant, heritable, phenotypic polymorphism and three ploidy levels including tetraploids (2n = 4x = 36), pentaploids (2n = 5x = 45) and hexaploids (2n = 6x = 54). Under water-deficit conditions, C. ciliaris shows plasticity in growth characteristics and aboveground biomass. This phenotypic plasticity has led to the identification of genotypic-associated responses conferring more productivity. This underlines the importance of conserving the genetic diversity of C. ciliaris in order to ensure the persistence of the vegetation cover in the arid ecosystems in which it occurs. Observations from cytogenetic and molecular data converge to underline the possibility of sexual reproduction, recombination and gene flow within and between populations of C. ciliaris. Genetic mechanisms, such as polyploidy, hybridisation between ploidy levels and apomixes, are generating and then maintaining the diversity of C. ciliaris. This review emphasises the role of polyploidy in the evolutionary development of C. ciliaris and how it may be a crucial factor for its conservation in some countries and its weedy nature in others.

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Section: Flow Cytometry For Ploidy Level Determinationmentioning

confidence: 96%

Section: Flow Cytometry For Ploidy Level Determinationmentioning

confidence: 99%

Section: Polyploidy Of Cenchrus Ciliarismentioning

confidence: 99%

Section: Flow Cytometry For Ploidy Level Determinationmentioning

confidence: 99%

Section: Flow Cytometry For Ploidy Level Determinationmentioning

confidence: 99%

See 3 more Smart Citations

The polyploid nature of Cenchrus ciliaris L. (Poaceae) has been overlooked: new insights for the conservation and invasion biology of this species – a review

et al. 2014

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DNA content and ploidy estimation of Festuca ovina accessions by flow cytometry

et al. 2020

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Background: Hard fescue (Festuca brevipila Tracey, 2n=6x=42) is a cool season turfgrass with a ne leaf texture that performs well under low-input management. Breeding and genetics studies of F. brevipila have been limited due to the complexity of its hexaploid genome. To advance our knowledge of F. brevipila genomics, we used PacBio isoform sequencing to develop a reference transcriptome for this species. Results: Here, we report the F. brevipila reference transcriptome generated from root, crown, leaf, and in orescence tissues. We obtained 59,510 full-length transcripts, of which 38,556 were non-redundant full-length transcripts. The longest and shortest transcripts were 11,487 and 58 bp, respectively. Distribution of synonymous distances among paralogs within F. brevipila suggested highly similar subgenomes that are di cult to distinguish from sequencing errors. To evaluate the phylogenetic relationships among F. brevipila and close relatives, we sequenced three additional transcriptomes using closely related species on an Illumina platform. The results of our phylotranscriptomic analysis supported the close relationships among F. brevipila (6x), Festuca ovina (4x), Festuca ovina subsp. ovina (2x), and Festuca valesiaca (2x), with high levels of discordance among gene trees. Conclusions: Overall, the F. brevipila PacBio Isoseq reference transcriptome provided the foundation for transcriptome studies and allowed breeders a resource for gene discovery in this important turfgrass species.

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The Use of Flow Cytometry for Estimating Genome Sizes and DNA Ploidy Levels in Plants

Loureiro

Čertner

Lučanová

et al. 2023

Methods in Molecular Biology

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Ploidy determination of buffel grass accessions in the USDA National Plant Germplasm System collection by flow cytometry

Cited by 21 publications

References 19 publications

The polyploid nature of Cenchrus ciliaris L. (Poaceae) has been overlooked: new insights for the conservation and invasion biology of this species – a review

The polyploid nature of Cenchrus ciliaris L. (Poaceae) has been overlooked: new insights for the conservation and invasion biology of this species – a review

DNA content and ploidy estimation of Festuca ovina accessions by flow cytometry

The Use of Flow Cytometry for Estimating Genome Sizes and DNA Ploidy Levels in Plants

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