Ploidy Effects in Isogenic Populations of Alfalfa

Meyers, Steven P.; Molin, William T.; Selman, Bruce R.; Schrader, L. E.

doi:10.1104/pp.70.6.1715

Cited by 6 publications

(1 citation statement)

References 21 publications

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“…Purification of CFls from spinach, lettuce, Swiss chard, pea, wheat, alfalfa, maize, various tobacco species, radish, petunia, Chlamydomonas reinhardii, Euglena gracilis, Spirodela oligorhiza and Dunaliella bardawil, by one, or a combination of the above described isolation procedures, have been reported [39,78,107,113,117,135,149,[150][151][152]. A detailed procedure for the purification of CF~ and its subunits is described by Jagendorf [71].…”

Section: Introductionmentioning

confidence: 99%

Photosynthetic ATPases: purification, properties, subunit isolation and function

Merchant

Selman

1985

Photosynth Res

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Photosynthetic coupling factor ATPases (F1-ATPases) generally censist of five subunits named α, β, γ, δ and ε in order of decreasing apparent molecular weight. The isolated enzyme has a molecular weight of between 390,000 to 400,000, with the five subunits probably occurring in a 3:3:1:1:1 ratio. Some photosynthetic F1 ATPases are inactive as isolated and require treatment with protease, heat or detergent in order to elicit ATPase activity. This activity is sensitive to inhibition by free divalent cations and appears to be more specific for Ca(2+) vs. Mg(2+) as the metal ion substrate chelate. This preference for Ca(2+) can be explained by the higher inhibition constant for inhibition of ATPase activity by free Ca(2+). Methods for the assay of a Mg-dependent ATPase activity have recently been described. These depend on the presence of organic solvents or detergents in the reaction mixture for assay. The molecular mechanism behind the expression of either the Ca- or Mg-ATPase activities is unknown. F1-ATPases function to couple proton efflux from thylakoid membranes or chromatophores to ATP synthesis. The isolated enzyme may thus also be assayed for the reconstitution of 'coupling activity' to membranes depleted of coupling factor 1.The functions of the five subunits in the complex have been deduced from the results of chemical modification and reconstitution studies. The δ subunit is required for the functional binding of the F1 to the F0. The active site is probably contained in the β (and α) subunit(s). The proposed functions for the γ and ε subunits are, however, still matters of controversy. Coupling factors from a wide variety of species including bacteria, algae, C3 and C4 plants, appear to be immunologically related. The β subunits are the most strongly related, although the α and γ subunits also show significant immunological cross-reactivity. DNA sequence analyses of the genes for the β subunit of CF1 have indicated that the primary sequence of this polypeptide is highly conserved. The genes for the polypeptides of CF1 appear to be located in two cellular compartments. The α, β and ε subunits are coded for on chloroplast DNA, whereas the γ and δ subunits are probably nuclear encoded. Experiments involving protein synthesis by isolated chloroplasts or protein synthesis in the presence of inhibitors specific for one or the other set of ribosomes in the cell suggest the existence of pools of unassembled CF1 subunits. These pools, if they do exist in vivo, probably make up no greater than 1% of the total CF1 content of the cell.

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Section: Introductionmentioning

confidence: 99%

Photosynthetic ATPases: purification, properties, subunit isolation and function

Merchant

Selman

1985

Photosynth Res

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Leaf Characteristics and Net Gas Exchange of Diploid and Autotetraploid Citrus

et al. 1997

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Effects of polyploidy on photosynthesis

1993

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In polyploid plants the photosynthetic rate per cell is correlated with the amount of DNA per cell. The photosynthetic rate per unit leaf area is the product of the rate per cell times the number of photosynthetic cells per unit area. Therefore, the photosynthetic rate per unit leaf area will increase if there is a less than proportional increase in cell volume at higher ploidal levels, or if cell packing is altered to allow more cells per unit leaf area. In autopolyploids (Medicago sativa, C3 species, and Pennisetum americanum, C4 species) there is a doubling of photosynthesis per cell and of cell volume in the tetraploid compared to the diploid. However, there is a proportional decrease in number of cells per unit leaf area with this increase in ploidy such that the rate of photosynthesis per leaf area does not change. There is more diversity in the relationship between ploidal level (gene dosage) and photosynthetic rates per unit leaf area in allopolyploids. This is likely to reflect the effects of natural selection on leaf anatomy, and novel genetic interactions from contributed genomes which can occur with allopolyploidy. In allopolyploid wheat (C3 species) a higher cell volume per unit DNA at the higher ploidal level is negatively correlated with photosynthesis rate per unit leaf area. Although photosynthesis per cell increases with ploidy, photosynthesis per leaf area decreases, being lowest in the allohexaploid, cultivated bread wheat (Triticum aestivum). Alternatively, doubling of photosynthetic rate per cell with doubling of DNA, with apparent natural selection for decreased cell volume per unit DNA, results in higher rates of photosynthesis per leaf area in octaploid compared to tetraploid Panicum virgatum (C4) which may be a case of allopolyploidy. Similar responses probably occur in Festuca arundinacea. Therefore, in some systems anatomical factors affecting photosynthesis are also affected by ploidal level. It is important to evaluate that component as well as determining the effect on biochemical processes. Current information on polyploidy and photosynthesis in several species is discussed with respect to anatomy, biochemistry and bases for expressing photosynthetic rates.

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Ploidy Effects in Isogenic Populations of Alfalfa

Cited by 6 publications

References 21 publications

Photosynthetic ATPases: purification, properties, subunit isolation and function

Photosynthetic ATPases: purification, properties, subunit isolation and function

Leaf Characteristics and Net Gas Exchange of Diploid and Autotetraploid Citrus

Effects of polyploidy on photosynthesis

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