Ploidy Level Affects Important Biomass Traits of Novel Shrub Willow (Salix) Hybrids

Serapiglia, Michelle J.; Gouker, Fred E.; Hart, James F.; Unda, Faride; Mansfield, Shawn D.; Stipanovic, Arthur J.; Smart, Lawrence B.

doi:10.1007/s12155-014-9521-x

Cited by 56 publications

(51 citation statements)

References 35 publications

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“…The stems of S. psammophila require flat stubble once every 3–5 years to allow growth to flourish and its branch residues contain high cellulose, hemicellulose and lignin contents of 55.45%, 18.98% and 25.98%, respectively89. The cellulose content of S. psammophila is higher than those reported in the study of Serapligia et al 10,. which included 75 diploid, triploid and tetraploid shrub willows with cellulose content ranging from 39.9% to 46.64%.…”

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confidence: 72%

De novo transcriptome assembly, development of EST-SSR markers and population genetic analyses for the desert biomass willow, Salix psammophila

Jia

Yang

Sun

et al. 2016

Sci Rep

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Salix psammophila, a sandy shrub known as desert willow, is regarded as a potential biomass feedstock and plays an important role in maintaining local ecosystems. However, a lack of genomic data and efficient molecular markers limit the study of its population evolution and genetic breeding. In this study, chromosome counts, flow cytometry and SSR analyses indicated that S. psammophila is tetraploid. A total of 6,346 EST-SSRs were detected based on 71,458 de novo assembled unigenes from transcriptome data. Twenty-seven EST-SSR markers were developed to evaluate the genetic diversity and population structure of S. psammophila from eight natural populations in Northern China. High levels of genetic diversity (mean 10.63 alleles per locus; mean HE 0.689) were dectected in S. psammophila. The weak population structure and little genetic differentiation (pairwise FST = 0.006–0.016) were found among Population 1-Population 7 (Pop1-Pop7; Inner Mongolia and Shaanxi), but Pop8 (Ningxia) was clearly separated from Pop1-Pop7 and moderate differentiation (pairwise FST = 0.045–0.055) was detected between them, which may be influenced by local habitat conditions. Molecular variance analyses indicated that most of the genetic variation (94.27%) existed within populations. These results provide valuable genetic informations for natural resource conservation and breeding programme optimisation of S. psammophila.

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confidence: 72%

De novo transcriptome assembly, development of EST-SSR markers and population genetic analyses for the desert biomass willow, Salix psammophila

Jia

Yang

Sun

et al. 2016

Sci Rep

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“…Recently, Serapiglia et al . demonstrated that triploid shrub willows produced higher biomass yield than their diploid and tetraploid parents23. As in many plants, polyploid breeding is also a highly desirable means for breeding elite willow cultivars.…”

Section: Discussionmentioning

confidence: 99%

“…These novel hybrids display significant variation in biomass production. Significant difference has been observed between ploidy level and growth in some willow species23. In general, triploid willows are more vigorous and produce higher yield than their diploid and tetraploid parents2425, and it has been demonstrated that the triploid and tetraploid willows possessed lower lignin content than the diploid genotypes23.…”

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confidence: 99%

“…Significant difference has been observed between ploidy level and growth in some willow species23. In general, triploid willows are more vigorous and produce higher yield than their diploid and tetraploid parents2425, and it has been demonstrated that the triploid and tetraploid willows possessed lower lignin content than the diploid genotypes23. Considering the significant effects of ploidy level on growth and wood composition, ploidy determination is critical for polyploid willow breeding programs26.…”

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confidence: 99%

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An analytical toolkit for polyploid willow discrimination

Guo

Hou

Yin

et al. 2016

Sci Rep

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Polyploid breeding is an important means for creating elite willow cultivars, and therefore provokes an active demand for discriminating the ploidy levels of natural willow stands. In this study, we established an analytical toolkit for polyploid willow identification by combining molecular markers and flow cytometry (FCM). A total of 10 single-copy fully informative SSRs were chosen for marker-aided selection based on a segregation test with a full-sib willow pedigree and a mutability test with a collection of natural willow stands. Aided by these molecular markers, we performed polyploid selection in two tree species and two shrub species of the genus Salix. The ploidy levels of the investigated samples were further examined using a flow cytometer. It was previously shown that results from marker-aided selection were consistent with those from FCM measurements. Based on ploidy level assessment in different willow species, it was found that tree willows were dominantly tetraploid, whereas shrub willows were most frequently diploid. With this analytical toolkit, polyploids can be rapidly screened from a large number of natural stands; thereafter, the exact ploidy levels of the polyploid candidates can be efficiently confirmed by FCM. This analytical toolkit will greatly enhance polyploid breeding programs for willows.

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“…The overall aim of the sustainable and economically efficient biomass conversion is to provide a biomass feedstock with optimized properties, best suited for further downstream conversion. Improvements in total biomass yield, biomass quality, and biomass energy content must be matched with the conversion process for which the willow is grown as a feedstock (Serapiglia et al 2015). Various aspects of the biomass quality could be of interest depending on the desired end-use.…”

Section: Introductionmentioning

confidence: 99%

Selection of optimal conversion path for willow biomass assisted by near infrared spectroscopy

Sandak¹,

Sandak²,

Waliszewska³

et al. 2017

iForest

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(4)Willow (Salix sp.) is one of the most common hardwood species suitable for short-rotation coppice. It can be converted to different products, including chemicals, fuels, fibers or furniture. It may also be used in agriculture and environmental engineering. Molecular composition of biomass and its physical properties highly influence effectiveness of its chemical, thermo-chemical or mechanical-chemical conversion. Therefore, it is challenging to provide biomass feedstock with optimized properties, best suited for further downstream conversion. The goal of this research was to establish a procedure for determination of the willow biomass optimal use cultivated in four different plantations in Poland. A special attention has been paid to the application of the near infrared spectroscopy for evaluation of biomass chemical composition and its physical properties. Near infrared spectroscopy (NIR) could be an alternative to standard analytical methods supporting the research and development of biomass production technologies. Partial least squares regression models for quantitative prediction of wood chemical components (lignin, cellulose, holocellulose, hemicellulose and extractives) and high heating values were developed. The residual prediction deviation (RPD) values confirm the applicability of chemometric models for screening in breeding programmes (for lignin, cellulose and extractives content) and for research in the case of high heating value. The analysis of NIR spectra highlighted several peculiarities in the chemical composition of the investigated willow clones. Finally, a knowledgebased expert system and a prototype automatic NIR system allowing the computation of a "suitability index" based on PLS models and dedicated to selection of optimal biomass conversion path, was developed.

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Ploidy Level Affects Important Biomass Traits of Novel Shrub Willow (Salix) Hybrids

Cited by 56 publications

References 35 publications

De novo transcriptome assembly, development of EST-SSR markers and population genetic analyses for the desert biomass willow, Salix psammophila

De novo transcriptome assembly, development of EST-SSR markers and population genetic analyses for the desert biomass willow, Salix psammophila

An analytical toolkit for polyploid willow discrimination

Selection of optimal conversion path for willow biomass assisted by near infrared spectroscopy

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