Ploidy Manipulation and Gynogenesis in Fishes: Cytogenetic and Fisheries Applications

Ihssen, P. E.; McKay, L.R.; McMillan, I.; Phillips, Ruth B.

doi:10.1577/1548-8659(1990)119<0698:pmagif>2.3.co;2

Cited by 232 publications

(127 citation statements)

References 91 publications

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“…The increase in cell size is accompanied by a decrease in cell number; the presence of a smaller number of larger cells has been established in a number of triploid tissues and organs as well as in triploid blood, where both the red and white cells are affected (Benfey 1991). Yriploidy also causes gametogenesis to be impaired with the result being that triploid salmonids are sterile (Dunham 1990;Ihssen et al 1990). Furthermore, in female triploids, reproductive endocrinology is altered such that these fish remain sexually immature throughout their lives (Benfey et al 1989).…”

Section: Introductionmentioning

confidence: 99%

Hemoglobin level, metabolic rate, opercular abduction rate and swimming efficiency in female triploid brook trout (Salvelinus fontinalis)

Stillwell

Benfey

1996

Fish Physiol Biochem

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Metabolic (oxygen consumption) rate, opercular abduction rate and tail beat frequency were determined in two strains of diploid and triploid female brook trout (Salvelinus fontinalis) while these fish swam at 0.37±0.02 body lengths per sec in a Blazka respirometer. Total blood hemoglobin level was also measured and opercular condition examined. Total blood hemoglobin levels in diploids and triploids were equal. The opercular abduction rate was the same in diploids and triploids (regardless of whether triploid opercular condition was good or poor) yet triploids had a lower oxygen consumption rate than diploids, indicating that triploids take up less oxygen than diploids per opercular cycle. Tail beat frequency, an indicator of swimming effort, was the same in diploids and triploids, suggesting that triploids require less oxygen than diploids for a similar swimming effort.

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Section: Introductionmentioning

confidence: 99%

Hemoglobin level, metabolic rate, opercular abduction rate and swimming efficiency in female triploid brook trout (Salvelinus fontinalis)

Stillwell

Benfey

1996

Fish Physiol Biochem

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“…In a variety of fish species, homozygous animals can be obtained using gynogenesis (all-female inheritance) or androgenesis (all-male inheritance) in only one generation (Ihssen et al, 1990). In androgenesis, the female genome is inactivated.…”

Section: Introductionmentioning

confidence: 99%

Androgenesis in the African catfish,Clarias gariepinus

Bongers¹,

Nguenga²,

Eding³

et al. 1995

Aquat. Living Resour.

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“…Chromosome set alterations yielding three or four sets of chromosomes (triploidy or tetraploidy) occur naturally in fishes and are well studied (Ihssen et al, 1990). Typically, production of tetraploidy is experimentally accomplished by interference with the first mitotic division by temperature shock or application of pressure or chemicals, which yields a single undivided cell containing the equivalent of two nuclei.…”

Section: Use Of Tetraploid Broodstocksmentioning

confidence: 99%

“…Androgenesis is another form of chromosome set manipulation that is well studied in fishes (Stoss, 1983;Ihssen et al, 1990). This natural phenomenon results in offspring that carry only two sets of paternal chromosomes.…”

Section: Utilization Of Androgenesismentioning

confidence: 99%

Cryopreservation in Aquarium Fishes

Tiersch

2001

Marine Biotechnology

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Few studies have addressed sperm cryopreservation in aquarium fishes (body sizes of 10 cm or less). There are several challenges inherent in developing cryopreservation procedures for these fishes. First, their small body size and sperm volume limit experimental replication and the numbers of treatments possible without pooling of samples. This hinders research, especially if many experimental variables are evaluated. The small sample volume necessitates identification of optimal sperm-to-egg ratios to maximize fertilization potential and places greater emphasis on increasing and maintaining sperm viability after thawing. Other technical problems include the use of 0.25-ml French straws, which increase difficulties in sample handling (automated straw fillers are more common for the 0.5-ml straw) and labeling. Sperm cryopreservation of live-bearing fishes (with internal fertilization) is essentially unexplored. The sperm of these fishes is sufficiently different in structure (e.g., head shape) and physiology (e.g., energy metabolism) from the sperm of other fishes that the need to develop specialized techniques is almost assured. The requirement for artificial insemination also introduces a new variable complicating the collection of data (e.g., assessing fertilization is not straightforward with internally held eggs). Cryopreservation in aquarium fishes will assist the development (e.g., through selective breeding), protection (e.g., through germplasm repositories), and distribution (e.g., through shipment of frozen sperm) of research lines and offers benefits for restoration of endangered species.

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Ploidy Manipulation and Gynogenesis in Fishes: Cytogenetic and Fisheries Applications

Cited by 232 publications

References 91 publications

Hemoglobin level, metabolic rate, opercular abduction rate and swimming efficiency in female triploid brook trout (Salvelinus fontinalis)

Hemoglobin level, metabolic rate, opercular abduction rate and swimming efficiency in female triploid brook trout (Salvelinus fontinalis)

Androgenesis in the African catfish,Clarias gariepinus

Cryopreservation in Aquarium Fishes

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