Ploidy manipulation using diploid sperm in the loach,<i>Misgurnus anguillicaudatus</i>: a review

Yoshikawa, Hiroyuki; Morishima, Kagayaki; Fujimoto, Takafumi; Arias‐Rodríguez, Lenin; Yamaha, Etsuro; Arai, Katsutoshi

doi:10.1111/j.1439-0426.2008.01129.x

Cited by 21 publications

(11 citation statements)

References 53 publications

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“…Unfortunately, our heat‐shock method was not fully successful, as a small percentage of normal diploid fish remained after the treatment. This suggests that other strategies, including fertilization using diploid sperm from tetraploids may be used in order to achieve 100% triploids, as have been observed in other species (Yoshikawa et al ; Yasui et al ). Moreover, some species may not be sterile despite being triploid (Felip et al ; Linhart et al ; Piferrer et al ; Fujimoto et al ) and therefore we must evaluate the reproductive performance of this species in future studies.…”

Section: Discussionmentioning

confidence: 87%

Triploid Induction in the Yellowtail Tetra, Astyanax altiparanae, Using Temperature Shock: Tools for Conservation and Aquaculture

Adamov

Nascimento

Maciel

et al. 2016

J World Aquaculture Soc

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Triploidization is an interesting tool to produce sterile fish. In the yellowtail tetra, Astyanax altiparanae, this can be applied for aquaculture and surrogate technologies. In this study, we compared the efficacy of cold (2 C) or heat shock (38 C, 40 C, and 42 C) on triploid induction in the yellowtail tetra. The eggs were treated with cold or heat shock, 2 min postfertilization (30 min in cold shock or 2 min in heat shock). Intact embryos served as the control group. Ploidy status was confirmed by karyotyping, flow cytometry, and nuclear diameter of erythrocytes. The hatching rate decreased after cold shock (12.69 ± 15.76%) and heat shock at 42 C (0.35 ± 0.69%) in comparison with the control group (63.19 ± 16.82%). At 38 C and 40 C, hatching rates (61.29 ± 17.73% and 61.75 ± 22.1%, respectively) were not decreased. Only one triploid arose at 38 C (1/80). At 40 C, a high number of triploids arose (72/78). At 42 C, very few embryos developed into the hatching stage. A large number of haploid individuals arose after cold shock (61/75), with only one triploid. Our results indicate that heat shocking of embryos at 40 C is optimum for triploid production in the yellowtail tetra.

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Section: Discussionmentioning

confidence: 87%

Triploid Induction in the Yellowtail Tetra, Astyanax altiparanae, Using Temperature Shock: Tools for Conservation and Aquaculture

Adamov

Nascimento

Maciel

et al. 2016

J World Aquaculture Soc

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“…Fertile triploid spermatozoa with genetic uniformity were found in triploid hybrid males of the Iberian minnow (Sousa-Santos et al, 2007), but such unreduced spermatozoa were not recognized in the present study. Unreduced spermatogenesis in the loach was reviewed by Yoshikawa et al (2008).…”

Section: Discussionmentioning

confidence: 99%

Genetic and reproductive potential of spermatozoa of diploid and triploid males obtained from interspecific hybridization ofMisgurnus anguillicaudatusfemale withM. mizolepismale

Fujimoto

Yasui

Yoshikawa

et al. 2008

Journal of Applied Ichthyology

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Short title: Diploid and triploid Misgurnus hybrid males SummaryDiploid and triploid interspecific hybrid male progeny obtained from mating Misgurnus anguillicaudatus with M. mizoleis were reported to have histologically fertile and sterile testes, respectively. However, their reproductive capacity is still unclear because mating tests have not been examined using mature hybrids. Here, we examined physiological and genetic characteristics of spermatozoa of diploid and triploid hybrids. In diploid hybrid males, 1n, 2n and 4n spermatozoa showing low motility were detected. However, spermatozoa of three diploid hybrid males could generate 2n larvae. Therefore, only 1n spermatozoa of diploid hybrid males was fertile to produce larva. The chromosomes of diploid hybrid males were transmitted to spermatozoa by random segregation between the homologous chromosomes because most larvae had one allele derived from both M. anguillicaudatus and M. mizolepis at all loci examined. In triploid hybrid males, spermatozoa could be categorized to three different types based on their ploidy status. Type 1: In the first and second males, sperm samples mainly comprised 6n spermatozoa. Motility and fertility were not recorded. Type 2: The third male gave a large proportion of 6n spermatozoa as well as a small proportion of 1n spermatozoa. Although no motility was observed, larvae arose from eggs inseminated with such spermatozoa. Type 3: In the fourth male, only 1n spermatozoa were detected and their motility was vigorous. When eggs were fertilized with such 1n spermatozoa, normal larvae hatched. 1n spermatozoa of the triploid hybrid male only included the M. anguillicaudatus genome. In Misgurnus fishes, diploid hybrid males exhibited semi-sterility or slight fertility. On the contrary, triploid hybrid males were sometime fertile due to the production of 1n spermatozoa by a kind of transformation of meiosis like meiotic hybridogenesis. Keywords: Misgurnus, hybrid male, sterility, triploid, spermatozoa IntroductionDiploid hybrids between two different fish species exhibit gonadal, gametic, or zygotic sterility depending on the combination of species (Chevassus, 1983). However, fertile hybrid fish often show alternative atypical reproduction, such as unreduced gametogenesis, gynogenesis, or hybridogenesis, especially in females (reviewed by Dawley, 1989). On the contrary, hybrid males are usually sterile in most cases, but a reproduction with fertile unreduced sperm was reported in the Iberian minnow with a natural hybrid origin (Alves et al., 1999) and common carp × crucian carp hybrids (Cherfas et al 1994; Liu et al., 2001;Sun et al., 2007).Interspecific hybridization has been regarded as a useful tool for aquaculture to improve growth rate as well as to add desirable traits (Bartley et al., 2001). Moreover, hybridization could be used as a means to prevent biological contamination, because sterile hybrid fishes are not risky to contaminate an indigenous gene pool by escaping from cages to natural environment (Bartley et al., 2001). Ho...

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“…Ploidy manipulation has been applied to improve economically important traits such as growth and meat quality for aquaculture (Arai, , ; Piferrer et al., ). Diploid gametes are regarded as a useful source or tool to manipulate ploidy (Yoshikawa et al., ). Auto‐triploid strains have been produced by crosses between normal diploid (haploid gametes) and artificially induced tetraploid (diploid gametes) in oyster (Guo et al., ) and salmonids (Chourrout et al., ).…”

Section: Introductionmentioning

confidence: 99%

Motility, morphology, mitochondria and ATP content of diploid spermatozoa from sex-reversed clonal diploid and neo-tetraploid loach,Misgurnus anguillicaudatus

et al. 2012

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Summary Diploid spermatozoa were obtained from sex‐reversed clonal diploid males and neo‐tetraploid which derived from normal diploid × natural tetraploid cross followed by the 2nd polar body release inhibition. The spermatozoa from sex‐reversed clonal diploid males exhibited a significant reduction in motility when compared with those from normal diploid and neo‐tetraploid males. Electron microscopy revealed that diploid spermatozoa from sex‐reversed clonal diploid and neo‐tetraploid males exhibited normal shape similar to haploid spermatozoa from normal diploid males, except for larger sperm‐head size and longer flagellum. Considering flow‐cytometric results, volume of mitochondrial mass per spermatozoon was also increased in proportion to the elevation of ploidy status from haploid to diploid sperm. Diploid spermatozoa from neo‐tetraploid increased the number of mitochondria and the ATP content, but those from sex‐reversed clonal diploid males did not show such results.

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Ploidy manipulation using diploid sperm in the loach,Misgurnus anguillicaudatus: a review

Cited by 21 publications

References 53 publications

Triploid Induction in the Yellowtail Tetra, Astyanax altiparanae, Using Temperature Shock: Tools for Conservation and Aquaculture

Triploid Induction in the Yellowtail Tetra, Astyanax altiparanae, Using Temperature Shock: Tools for Conservation and Aquaculture

Genetic and reproductive potential of spermatozoa of diploid and triploid males obtained from interspecific hybridization ofMisgurnus anguillicaudatusfemale withM. mizolepismale

Motility, morphology, mitochondria and ATP content of diploid spermatozoa from sex-reversed clonal diploid and neo-tetraploid loach,Misgurnus anguillicaudatus

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