Plugging In Motoneurons

Wong, Wei

doi:10.1126/scisignal.262ec96

Cited by 1 publication

(2 citation statements)

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“…[1,2] Real-time monitoring of these molecules is therefore important to gain insights into cell physiology and how cells respond to changes in their environment such as nutrient availability, environmental toxins, or drug exposure. [3,4] Degeneration and death of neurons leading to a decline of cognitive and motor functions can be caused by a variety of factors, which is the reason why neurodegenerative disorders are currently incurable and represent a significant burden to society. [5,6] To understand such complex mechanisms and develop effective treatments, highly sensitive biosensors that detect temporarily-and spatially-resolved biomolecular changes at the cellular level are of great importance.…”

Section: Introductionmentioning

confidence: 99%

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Smart Slides for Optical Monitoring of Cellular Processes

Ackermann,

Reger,

Jung

et al. 2023

Adv Funct Materials

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The molecules released by cells are a fingerprint of their current state. Methods that measure them with high spatial and temporal resolution may provide valuable insights into cell physiology and diseases. Here, a nanosensor coating is developed that transforms standard cell culture materials/dishes into “Smart Slides” capable of optically monitoring biochemical efflux from cells. For this purpose, single wall carbon nanotubes (SWCNTs) that are fluorescent in the beneficial near‐infrared (NIR, 850 – 1700 nm) window are used. They are chemically tailored to detect the neurotransmitter dopamine by a change in fluorescence intensity. These nanosensors are spin‐coated on glass substrates and it is shown that such sensor layers can be sterilized by UV light and can be stored in dry condition or buffer for at least 6 weeks and have little influence on cell viability. The optimal sensor density to maximize sensitivity is also identified. Finally, these substrates are used to image dopamine release from neuronal cells cultivated on top in the presence of various psychotropic substances, which represents a system to test pharmaceuticals for neurological or neurodegenerative diseases. Therefore, Smart Slides are a powerful tool to monitor cellular processes in cell culture systems.

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Section: Introductionmentioning

confidence: 99%

“…[ 1,2 ] Real‐time monitoring of these molecules is therefore important to gain insights into cell physiology and how cells respond to changes in their environment such as nutrient availability, environmental toxins, or drug exposure. [ 3,4 ]…”

Section: Introductionmentioning

confidence: 99%